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Literature summary extracted from

  • Miyazawa, D.; Ikemoto, A.; Fujii, Y.; Okuyama, H.
    Partial purification and characterization of phosphatidic acid-specific phospholipase A1 in porcine platelet membranes (2003), Biochim. Biophys. Acta, 1631, 17-25.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.32 Triton X-100 maximal activity at 0.025% Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.32 diisopropyl fluorophosphate IC50: 8 mM Sus scrofa
3.1.1.32 dithiothreitol 1 mM, complete inhibition Sus scrofa
3.1.1.32 DNA containing thymine glycol 0.05 mM, 50% inhibition Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.32 membrane
-
 Sus scrofa 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.32 phosphatidylcholine + H2O Sus scrofa
-
 2-acylglycerophosphocholine + a carboxylate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.32 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.32 Triton X-100, Blue-Toyopearl, Red-Toyopearl, DEAE-Toyopearl, Green-agarose, Brown-agarose, Polylysine-agarose, Palmitoyl-CoA-agarose, Blue-5PW Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.32 platelet
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.32 0.42
-
-
 Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.32 1-acyl-lysophosphtidic acid + H2O 7% of activity with phosphatidic acid Sus scrofa glycerol-3-phosphate + a carboxylate
-
 ?
3.1.1.32 phosphatidic acid + H2O
-
 Sus scrofa lysophosphatidic acid + a carboxylate
-
 ?
3.1.1.32 phosphatidylcholine + H2O
-
 Sus scrofa 2-acylglycerophosphocholine + a carboxylate
-
 ?
3.1.1.32 phosphatidylcholine + H2O 7% of activity with phosphatidic acid, at 0.1% Triton X-100 Sus scrofa 2-acylglycerophosphocholine + a carboxylate
-
 ?
3.1.1.32 phosphatidylinositol + H2O 1% of activity with phosphatidic acid, at 0.1% Triton X-100 Sus scrofa lysophosphatidylinositol + a carboxylate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.32 PA-PLA1
-
 Sus scrofa
3.1.1.32 phosphatidic acid-specific phospholipase A1
-
 Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.32 8
-
-
 Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.32 6.2 9.5 approx. 6% of maximal activity at pH 6.2, approx. 23% of maximal activity at pH 9.5 Sus scrofa

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.1.32 8
-
 IC50: 8 mM Sus scrofa diisopropyl fluorophosphate