BRENDA - Enzyme Database

Partial purification and characterization of phosphatidic acid-specific phospholipase A1 in porcine platelet membranes

Miyazawa, D.; Ikemoto, A.; Fujii, Y.; Okuyama, H.; Biochim. Biophys. Acta 1631, 17-25 (2003)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.32
Triton X-100
maximal activity at 0.025%
Sus scrofa
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.32
diisopropyl fluorophosphate
IC50: 8 mM
Sus scrofa
3.1.1.32
dithiothreitol
1 mM, complete inhibition
Sus scrofa
3.1.1.32
DNA containing thymine glycol
0.05 mM, 50% inhibition
Sus scrofa
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.32
membrane
-
Sus scrofa
16020
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.1.32
phosphatidylcholine + H2O
Sus scrofa
-
2-acylglycerophosphocholine + a carboxylate
-
Sus scrofa
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.32
Sus scrofa
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.1.32
Triton X-100, Blue-Toyopearl, Red-Toyopearl, DEAE-Toyopearl, Green-agarose, Brown-agarose, Polylysine-agarose, Palmitoyl-CoA-agarose, Blue-5PW
Sus scrofa
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.32
platelet
-
Sus scrofa
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.1.32
0.42
-
-
Sus scrofa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.32
1-acyl-lysophosphtidic acid + H2O
7% of activity with phosphatidic acid
650395
Sus scrofa
glycerol-3-phosphate + a carboxylate
-
650395
Sus scrofa
?
3.1.1.32
phosphatidic acid + H2O
-
650395
Sus scrofa
lysophosphatidic acid + a carboxylate
-
650395
Sus scrofa
?
3.1.1.32
phosphatidylcholine + H2O
-
650395
Sus scrofa
2-acylglycerophosphocholine + a carboxylate
-
650395
Sus scrofa
?
3.1.1.32
phosphatidylcholine + H2O
7% of activity with phosphatidic acid, at 0.1% Triton X-100
650395
Sus scrofa
2-acylglycerophosphocholine + a carboxylate
-
650395
Sus scrofa
?
3.1.1.32
phosphatidylinositol + H2O
1% of activity with phosphatidic acid, at 0.1% Triton X-100
650395
Sus scrofa
lysophosphatidylinositol + a carboxylate
-
650395
Sus scrofa
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.32
8
-
-
Sus scrofa
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.1.32
6.2
9.5
approx. 6% of maximal activity at pH 6.2, approx. 23% of maximal activity at pH 9.5
Sus scrofa
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
3.1.1.32
8
-
IC50: 8 mM
Sus scrofa
diisopropyl fluorophosphate
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.32
Triton X-100
maximal activity at 0.025%
Sus scrofa
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
3.1.1.32
8
-
IC50: 8 mM
Sus scrofa
diisopropyl fluorophosphate
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.32
diisopropyl fluorophosphate
IC50: 8 mM
Sus scrofa
3.1.1.32
dithiothreitol
1 mM, complete inhibition
Sus scrofa
3.1.1.32
DNA containing thymine glycol
0.05 mM, 50% inhibition
Sus scrofa
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.32
membrane
-
Sus scrofa
16020
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.1.32
phosphatidylcholine + H2O
Sus scrofa
-
2-acylglycerophosphocholine + a carboxylate
-
Sus scrofa
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.32
Triton X-100, Blue-Toyopearl, Red-Toyopearl, DEAE-Toyopearl, Green-agarose, Brown-agarose, Polylysine-agarose, Palmitoyl-CoA-agarose, Blue-5PW
Sus scrofa
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.32
platelet
-
Sus scrofa
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.1.32
0.42
-
-
Sus scrofa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.32
1-acyl-lysophosphtidic acid + H2O
7% of activity with phosphatidic acid
650395
Sus scrofa
glycerol-3-phosphate + a carboxylate
-
650395
Sus scrofa
?
3.1.1.32
phosphatidic acid + H2O
-
650395
Sus scrofa
lysophosphatidic acid + a carboxylate
-
650395
Sus scrofa
?
3.1.1.32
phosphatidylcholine + H2O
-
650395
Sus scrofa
2-acylglycerophosphocholine + a carboxylate
-
650395
Sus scrofa
?
3.1.1.32
phosphatidylcholine + H2O
7% of activity with phosphatidic acid, at 0.1% Triton X-100
650395
Sus scrofa
2-acylglycerophosphocholine + a carboxylate
-
650395
Sus scrofa
?
3.1.1.32
phosphatidylinositol + H2O
1% of activity with phosphatidic acid, at 0.1% Triton X-100
650395
Sus scrofa
lysophosphatidylinositol + a carboxylate
-
650395
Sus scrofa
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.32
8
-
-
Sus scrofa
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.1.32
6.2
9.5
approx. 6% of maximal activity at pH 6.2, approx. 23% of maximal activity at pH 9.5
Sus scrofa