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Literature summary extracted from

  • Moller, M.G.; Taylor, C.; Rasmussen, S.K.; Holm, P.B.
    Molecular cloning and characterization of two genes encoding asparagine synthetase in barley (Hordeum vulgare L.) (2003), Biochim. Biophys. Acta, 1628, 123-132.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.5.4 HvAS1, from leaves, located to the long arm of chromosome 5, sequencing Hordeum vulgare
6.3.5.4 HvAS2, from leaves, located to the short arm of chromosome 3, sequencing Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.5.4 65000
-
 x * 65500, amino acid sequence calculation, x * 65000, Western blot analysis, HvAS1 Hordeum vulgare
6.3.5.4 65200
-
 x * 65200, HvAS2, amino acid sequence calculation Hordeum vulgare
6.3.5.4 65500
-
 x * 65500, amino acid sequence calculation, x * 65000, Western blot analysis, HvAS1 Hordeum vulgare

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.5.4 additional information Hordeum vulgare under normal growth conditions HvAS1 gene seems to be important in roots where nitrogen is assimilated into asparagine for long-distance transport within the plant ?
-
 ?
6.3.5.4 additional information Hordeum vulgare under normal growth conditions HvAS2 acts as a housekeeping gene in the leaves ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.4 Hordeum vulgare Q84LA5 cv. Alexis, HvAS2, two AS genes: HvAS1 and HvAS2
-
6.3.5.4 Hordeum vulgare Q93XP9 cv. Alexis, HvAS1, two AS genes: HvAS1 and HvAS2
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
6.3.5.4 additional information no posttranslational regulation of HvAS1 Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.5.4 leaf expression of HvAS1, higher mRNA levels in younger leaves than in older leaves, induced by dark treatment, induction seems to require a dramatic change in the C/N ratio since no diurnal variation is observed and up-regulation of transcription only occurs after 10 h of darkness Hordeum vulgare
-
6.3.5.4 leaf expression of HvAS2 Hordeum vulgare
-
6.3.5.4 additional information expression pattern, dark treatment of plants increases expression of HvAS1 Hordeum vulgare
-
6.3.5.4 additional information expression pattern, no expression of HvAS2 in roots Hordeum vulgare
-
6.3.5.4 root low-level expression of HvAS1, unaffected by light Hordeum vulgare
-
6.3.5.4 stem expression of HvAS1 Hordeum vulgare
-
6.3.5.4 stem expression of HvAS2 Hordeum vulgare
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.4 ATP + L-aspartate + L-glutamine
-
 Hordeum vulgare AMP + diphosphate + L-asparagine + L-glutamate
-
 ?
6.3.5.4 additional information under normal growth conditions HvAS1 gene seems to be important in roots where nitrogen is assimilated into asparagine for long-distance transport within the plant Hordeum vulgare ?
-
 ?
6.3.5.4 additional information under normal growth conditions HvAS2 acts as a housekeeping gene in the leaves Hordeum vulgare ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.5.4 ? x * 65200, HvAS2, amino acid sequence calculation Hordeum vulgare
6.3.5.4 ? x * 65500, amino acid sequence calculation, x * 65000, Western blot analysis, HvAS1 Hordeum vulgare

Synonyms

EC Number Synonyms Comment Organism
6.3.5.4 AS
-
 Hordeum vulgare
6.3.5.4 HvAS1 protein Hordeum vulgare Hordeum vulgare
6.3.5.4 HvAS2 protein Hordeum vulgare Hordeum vulgare

pI Value

EC Number Organism Comment pI Value Maximum pI Value
6.3.5.4 Hordeum vulgare HvAS2, theoretical pI
-
 5.92
6.3.5.4 Hordeum vulgare HvAS1, theoretical pI
-
 6.14