Data extracted from this reference:
Activating Compound
3.13.1.3
additional information
no activation by metal ions
Rhodococcus erythropolis
Cloned(Commentary)
3.13.1.3
gene dszZ, DNA sequence determination, cooverexpression in Escherichia coli with chaperonin genes groEL and groES at 25°C
Rhodococcus erythropolis
Engineering
3.13.1.3
C26S
site-directed mutagenesis of the only cysteine residue results in an completely inactive mutant
Rhodococcus erythropolis
Inhibitors
3.13.1.3
2,2'-Dihydroxybiphenyl
competitive
Rhodococcus erythropolis
3.13.1.3
2,3-Dihydroxybiphenyl
competitive
Rhodococcus erythropolis
3.13.1.3
2-Hydroxybiphenyl
competitive, product inhibition
Rhodococcus erythropolis
3.13.1.3
Ag+
strong inhibition at 1 mM
Rhodococcus erythropolis
3.13.1.3
Cu2+
strong inhibition at 1 mM
Rhodococcus erythropolis
3.13.1.3
DTNB
strong inhibition at 1 mM
Rhodococcus erythropolis
3.13.1.3
Hg2+
strong inhibition at 1 mM
Rhodococcus erythropolis
3.13.1.3
additional information
no inhibition by product sulfite, no inhibition by chelating agents EDTA, 1,10-phenanthroline, and 8-hydroxyquinone
Rhodococcus erythropolis
3.13.1.3
N-ethylmaleimide
strong inhibition at 1 mM
Rhodococcus erythropolis
3.13.1.3
p-chloromercuribenzoate
strong inhibition at 1 mM
Rhodococcus erythropolis
KM Value [mM]
3.13.1.3
0.0082
2'-hydroxybiphenyl-2-sulfinate
pH 7.0, 28°C
Rhodococcus erythropolis
3.13.1.3
0.062
2-phenylbenzenesulfinate
pH 7.0, 28°C
Rhodococcus erythropolis
Metals/Ions
3.13.1.3
additional information
no metal ions required
Rhodococcus erythropolis
Molecular Weight [Da]
3.13.1.3
40000
1 * 40000, SDS-PAGE
Rhodococcus erythropolis
3.13.1.3
43000
gel filtration
Rhodococcus erythropolis
Natural Substrates/ Products (Substrates)
3.13.1.3
2'-hydroxybiphenyl-2-sulfinate + H2O
Rhodococcus erythropolis
2-hydroxybiphenyl + sulfite
Rhodococcus erythropolis
?
3.13.1.3
2'-hydroxybiphenyl-2-sulfinate + H2O
Rhodococcus erythropolis KA2-5-1
2-hydroxybiphenyl + sulfite
Rhodococcus erythropolis KA2-5-1
?
Organism
3.1.2.24
Rhodococcus erythropolis
3.1.2.24
Rhodococcus erythropolis KA2-5-1
3.13.1.3
Rhodococcus erythropolis
3.13.1.3
Rhodococcus erythropolis KA2-5-1
Purification (Commentary)
3.13.1.3
recombinant 7.8fold from Escherichia coli, and native from strain KA2-5-1 160fold, to homogeneity
Rhodococcus erythropolis
Reaction
3.13.1.3
2'-hydroxybiphenyl-2-sulfinate + H2O = 2-hydroxybiphenyl + sulfite
Cys26 is essential for activity
Rhodococcus erythropolis
Specific Activity [micromol/min/mg]
3.13.1.3
0.18
purified native enzyme
Rhodococcus erythropolis
3.13.1.3
0.207
purified recombinant enzyme
Rhodococcus erythropolis
Substrates and Products (Substrate)
3.13.1.3
2'-hydroxybiphenyl-2-sulfinate + H2O
650387
Rhodococcus erythropolis
2-hydroxybiphenyl + sulfite
650387
Rhodococcus erythropolis
?
3.13.1.3
2'-hydroxybiphenyl-2-sulfinate + H2O
highly specific for
650387
Rhodococcus erythropolis
2-hydroxybiphenyl + sulfite
650387
Rhodococcus erythropolis
?
3.13.1.3
2'-hydroxybiphenyl-2-sulfinate + H2O
650387
Rhodococcus erythropolis KA2-5-1
2-hydroxybiphenyl + sulfite
650387
Rhodococcus erythropolis KA2-5-1
?
3.13.1.3
2'-hydroxybiphenyl-2-sulfinate + H2O
highly specific for
650387
Rhodococcus erythropolis KA2-5-1
2-hydroxybiphenyl + sulfite
650387
Rhodococcus erythropolis KA2-5-1
?
3.13.1.3
2-phenylbenzenesulfinate + H2O
650387
Rhodococcus erythropolis
biphenyl + sulfite
650387
Rhodococcus erythropolis
?
3.13.1.3
2-phenylbenzenesulfinate + H2O
650387
Rhodococcus erythropolis KA2-5-1
biphenyl + sulfite
650387
Rhodococcus erythropolis KA2-5-1
?
Subunits
3.13.1.3
monomer
1 * 40000, SDS-PAGE
Rhodococcus erythropolis
Synonyms
3.13.1.3
2'-hydroxybiphenyl-2-sulfinate desulfinase
Rhodococcus erythropolis
3.13.1.3
DszB
Rhodococcus erythropolis
Temperature Optimum [°C]
3.13.1.3
35
recombinant enzyme
Rhodococcus erythropolis
Temperature Stability [°C]
3.13.1.3
28
recombinant enzyme, stable
Rhodococcus erythropolis
3.13.1.3
30
recombinant enzyme, rapid loss of activity above, inactivation after 4 h
Rhodococcus erythropolis
Turnover Number [1/s]
3.1.2.24
0.103
2-phenylbenzenesulfinate
pH 7.0, 28°C
Rhodococcus erythropolis
3.1.2.24
0.123
2'-hydroxybiphenyl-2-sulfinate
pH 7.0, 28°C
Rhodococcus erythropolis
pH Optimum
3.13.1.3
7.5
about, recombinant enzyme
Rhodococcus erythropolis
Cofactor
3.13.1.3
additional information
no cofactor required
Rhodococcus erythropolis
Ki Value [mM]
3.13.1.3
0.25
2-Hydroxybiphenyl
pH 7.0, 28°C, recombinant enzyme
Rhodococcus erythropolis
3.13.1.3
0.4
2,3-Dihydroxybiphenyl
pH 7.0, 28°C, recombinant enzyme
Rhodococcus erythropolis
3.13.1.3
2
2,2'-Dihydroxybiphenyl
pH 7.0, 28°C, recombinant enzyme
Rhodococcus erythropolis
Activating Compound (protein specific)
3.13.1.3
additional information
no activation by metal ions
Rhodococcus erythropolis
Cloned(Commentary) (protein specific)
3.13.1.3
gene dszZ, DNA sequence determination, cooverexpression in Escherichia coli with chaperonin genes groEL and groES at 25°C
Rhodococcus erythropolis
Cofactor (protein specific)
3.13.1.3
additional information
no cofactor required
Rhodococcus erythropolis
Engineering (protein specific)
3.13.1.3
C26S
site-directed mutagenesis of the only cysteine residue results in an completely inactive mutant
Rhodococcus erythropolis
Inhibitors (protein specific)
3.13.1.3
2,2'-Dihydroxybiphenyl
competitive
Rhodococcus erythropolis
3.13.1.3
2,3-Dihydroxybiphenyl
competitive
Rhodococcus erythropolis
3.13.1.3
2-Hydroxybiphenyl
competitive, product inhibition
Rhodococcus erythropolis
3.13.1.3
Ag+
strong inhibition at 1 mM
Rhodococcus erythropolis
3.13.1.3
Cu2+
strong inhibition at 1 mM
Rhodococcus erythropolis
3.13.1.3
DTNB
strong inhibition at 1 mM
Rhodococcus erythropolis
3.13.1.3
Hg2+
strong inhibition at 1 mM
Rhodococcus erythropolis
3.13.1.3
additional information
no inhibition by product sulfite, no inhibition by chelating agents EDTA, 1,10-phenanthroline, and 8-hydroxyquinone
Rhodococcus erythropolis
3.13.1.3
N-ethylmaleimide
strong inhibition at 1 mM
Rhodococcus erythropolis
3.13.1.3
p-chloromercuribenzoate
strong inhibition at 1 mM
Rhodococcus erythropolis
Ki Value [mM] (protein specific)
3.13.1.3
0.25
2-Hydroxybiphenyl
pH 7.0, 28°C, recombinant enzyme
Rhodococcus erythropolis
3.13.1.3
0.4
2,3-Dihydroxybiphenyl
pH 7.0, 28°C, recombinant enzyme
Rhodococcus erythropolis
3.13.1.3
2
2,2'-Dihydroxybiphenyl
pH 7.0, 28°C, recombinant enzyme
Rhodococcus erythropolis
KM Value [mM] (protein specific)
3.13.1.3
0.0082
2'-hydroxybiphenyl-2-sulfinate
pH 7.0, 28°C
Rhodococcus erythropolis
3.13.1.3
0.062
2-phenylbenzenesulfinate
pH 7.0, 28°C
Rhodococcus erythropolis
Metals/Ions (protein specific)
3.13.1.3
additional information
no metal ions required
Rhodococcus erythropolis
Molecular Weight [Da] (protein specific)
3.13.1.3
40000
1 * 40000, SDS-PAGE
Rhodococcus erythropolis
3.13.1.3
43000
gel filtration
Rhodococcus erythropolis
Natural Substrates/ Products (Substrates) (protein specific)
3.13.1.3
2'-hydroxybiphenyl-2-sulfinate + H2O
Rhodococcus erythropolis
2-hydroxybiphenyl + sulfite
Rhodococcus erythropolis
?
3.13.1.3
2'-hydroxybiphenyl-2-sulfinate + H2O
Rhodococcus erythropolis KA2-5-1
2-hydroxybiphenyl + sulfite
Rhodococcus erythropolis KA2-5-1
?
Purification (Commentary) (protein specific)
3.13.1.3
recombinant 7.8fold from Escherichia coli, and native from strain KA2-5-1 160fold, to homogeneity
Rhodococcus erythropolis
Specific Activity [micromol/min/mg] (protein specific)
3.13.1.3
0.18
purified native enzyme
Rhodococcus erythropolis
3.13.1.3
0.207
purified recombinant enzyme
Rhodococcus erythropolis
Substrates and Products (Substrate) (protein specific)
3.13.1.3
2'-hydroxybiphenyl-2-sulfinate + H2O
650387
Rhodococcus erythropolis
2-hydroxybiphenyl + sulfite
650387
Rhodococcus erythropolis
?
3.13.1.3
2'-hydroxybiphenyl-2-sulfinate + H2O
highly specific for
650387
Rhodococcus erythropolis
2-hydroxybiphenyl + sulfite
650387
Rhodococcus erythropolis
?
3.13.1.3
2'-hydroxybiphenyl-2-sulfinate + H2O
650387
Rhodococcus erythropolis KA2-5-1
2-hydroxybiphenyl + sulfite
650387
Rhodococcus erythropolis KA2-5-1
?
3.13.1.3
2'-hydroxybiphenyl-2-sulfinate + H2O
highly specific for
650387
Rhodococcus erythropolis KA2-5-1
2-hydroxybiphenyl + sulfite
650387
Rhodococcus erythropolis KA2-5-1
?
3.13.1.3
2-phenylbenzenesulfinate + H2O
650387
Rhodococcus erythropolis
biphenyl + sulfite
650387
Rhodococcus erythropolis
?
3.13.1.3
2-phenylbenzenesulfinate + H2O
650387
Rhodococcus erythropolis KA2-5-1
biphenyl + sulfite
650387
Rhodococcus erythropolis KA2-5-1
?
Subunits (protein specific)
3.13.1.3
monomer
1 * 40000, SDS-PAGE
Rhodococcus erythropolis
Temperature Optimum [°C] (protein specific)
3.13.1.3
35
recombinant enzyme
Rhodococcus erythropolis
Temperature Stability [°C] (protein specific)
3.13.1.3
28
recombinant enzyme, stable
Rhodococcus erythropolis
3.13.1.3
30
recombinant enzyme, rapid loss of activity above, inactivation after 4 h
Rhodococcus erythropolis
Turnover Number [1/s] (protein specific)
3.1.2.24
0.103
2-phenylbenzenesulfinate
pH 7.0, 28°C
Rhodococcus erythropolis
3.1.2.24
0.123
2'-hydroxybiphenyl-2-sulfinate
pH 7.0, 28°C
Rhodococcus erythropolis
pH Optimum (protein specific)
3.13.1.3
7.5
about, recombinant enzyme
Rhodococcus erythropolis