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Literature summary extracted from
- Cloning, expression and mutagenesis of a subunit contact of rabbit muscle-specific (betabeta) enolase (2002), Biochim. Biophys. Acta, 1597, 311-319.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.1.11 | expression of recombinant wild-type and mutant enzymes in Escherichia coli | Oryctolagus cuniculus |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 4.2.1.11 | E414L | this mutant has the same activity than the wild-type enzyme | Oryctolagus cuniculus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.11 | NaClO4 | E414L mutant is more sensitive to inactivation than the wild-type enzyme | Oryctolagus cuniculus |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.11 | 43 | - |
phosphoenolpyruvate | E414L mutant enzyme, pH 7.1, 25ºC | Oryctolagus cuniculus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.11 | 46940 | - |
mass spectrometry | Oryctolagus cuniculus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.11 | Oryctolagus cuniculus | P25704 | rabbit | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.11 | purification of the wild-type and a mutant enzyme cloned in Escherichia coli using ammonium sulfate precipitation and Sephadex chromatography | Oryctolagus cuniculus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.11 | 67 | - |
recombinant wild-type enzyme, pH 7.1, 25ºC | Oryctolagus cuniculus |
| 4.2.1.11 | 77 | - |
recombinant E414L mutant, pH 7.1, 25ºC | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.11 | 2-phospho-D-glycerate | - |
Oryctolagus cuniculus | phosphoenolpyruvate | - |
r | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.1.11 | enolase | - |
Oryctolagus cuniculus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.11 | 4 | - |
both the wild-type and the E414L mutant are stable as suspensions in ammonium sulfate | Oryctolagus cuniculus |
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Release 2023.1 (January 2023)
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