BRENDA - Enzyme Database

Cloning, expression and mutagenesis of a subunit contact of rabbit muscle-specific (betabeta) enolase

Kornblatt, M.J.; Zheng, S.X.; Lamande, N.; Lazar, M.; Biochim. Biophys. Acta 1597, 311-319 (2002)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.11
expression of recombinant wild-type and mutant enzymes in Escherichia coli
Oryctolagus cuniculus
Engineering
EC Number
Amino acid exchange
Commentary
Organism
4.2.1.11
E414L
this mutant has the same activity than the wild-type enzyme
Oryctolagus cuniculus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
NaClO4
E414L mutant is more sensitive to inactivation than the wild-type enzyme
Oryctolagus cuniculus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
43
-
phosphoenolpyruvate
E414L mutant enzyme, pH 7.1, 25C
Oryctolagus cuniculus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
46940
-
mass spectrometry
Oryctolagus cuniculus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Oryctolagus cuniculus
P25704
rabbit
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
purification of the wild-type and a mutant enzyme cloned in Escherichia coli using ammonium sulfate precipitation and Sephadex chromatography
Oryctolagus cuniculus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
4.2.1.11
67
-
recombinant wild-type enzyme, pH 7.1, 25C
Oryctolagus cuniculus
4.2.1.11
77
-
recombinant E414L mutant, pH 7.1, 25C
Oryctolagus cuniculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
650385
Oryctolagus cuniculus
phosphoenolpyruvate
-
650385
Oryctolagus cuniculus
r
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
4.2.1.11
4
-
both the wild-type and the E414L mutant are stable as suspensions in ammonium sulfate
Oryctolagus cuniculus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
expression of recombinant wild-type and mutant enzymes in Escherichia coli
Oryctolagus cuniculus
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
4.2.1.11
E414L
this mutant has the same activity than the wild-type enzyme
Oryctolagus cuniculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
NaClO4
E414L mutant is more sensitive to inactivation than the wild-type enzyme
Oryctolagus cuniculus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
43
-
phosphoenolpyruvate
E414L mutant enzyme, pH 7.1, 25C
Oryctolagus cuniculus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
46940
-
mass spectrometry
Oryctolagus cuniculus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
purification of the wild-type and a mutant enzyme cloned in Escherichia coli using ammonium sulfate precipitation and Sephadex chromatography
Oryctolagus cuniculus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
4.2.1.11
67
-
recombinant wild-type enzyme, pH 7.1, 25C
Oryctolagus cuniculus
4.2.1.11
77
-
recombinant E414L mutant, pH 7.1, 25C
Oryctolagus cuniculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
650385
Oryctolagus cuniculus
phosphoenolpyruvate
-
650385
Oryctolagus cuniculus
r
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
4.2.1.11
4
-
both the wild-type and the E414L mutant are stable as suspensions in ammonium sulfate
Oryctolagus cuniculus