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Literature summary extracted from
- pH, inhibitor, and substrate specificity studies on Escherichia coli penicillin-binding protein 5 (2002), Biochim. Biophys. Acta, 1597, 292-300.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.4.16.4 | without stabilization dilution of the enzyme to 10 nM results in a 97% loss of activity, whereas with stabilization by bovine serum albumin less than 10% loss of activity is observed | Escherichia coli |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.16.4 | diisopropyl fluorophosphate | 1 mM, 28% inhibition | Escherichia coli |  |
| 3.4.16.4 | formaldehyde | 1 mM, 53% inhibition | Escherichia coli | |
| 3.4.16.4 | iodoacetamide | 1 mM, 20% inhibition | Escherichia coli | |
| 3.4.16.4 | N-Chlorosuccinimide | 1 mM, 50% inhibition | Escherichia coli | |
| 3.4.16.4 | Nalpha-tert-butoxycarbonyl-Nepsilon-benzyloxycarbonyl-L-Lys-D-Ala-D-Ala | substrate inhibition above 30 mM | Escherichia coli | |
| 3.4.16.4 | NEM | 1 mM, complete inhibition | Escherichia coli | |
| 3.4.16.4 | p-hydroxymercuribenzoate | 1 mM, complete inhibition | Escherichia coli | |
| 3.4.16.4 | phenylboronic acid | 1 mM, 26% inhibition | Escherichia coli | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.16.4 | Escherichia coli | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.16.4 | - |
Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.16.4 | Nalpha,Nepsilon-diacetyl-Lys-D-Ala-D-Ala + H2O | - |
Escherichia coli | Nalpha,Nepsilon-diacetyl-Lys-D-Ala + D-Ala | - |
? | |
| 3.4.16.4 | Nalpha-tert-butoxycarbonyl-Nepsilon-benzyloxycarbonyl-L-Lys-D-Ala-D-Ala + H2O | - |
Escherichia coli | Nalpha-tert-butoxycarbonyl-Nepsilon-benzyloxycarbonyl-L-Lys-D-Ala + D-Ala | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.16.4 | PBP 5 | - |
Escherichia coli |
| 3.4.16.4 | penicillin-binding protein 5 | - |
Escherichia coli |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.16.4 | 9 | 10 | - |
Escherichia coli |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.16.4 | 8 | 11 | pH 8: about 50% of maximal activity, pH 11: about 50% of maximal activity | Escherichia coli |
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