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Literature summary extracted from

  • Capila, I.; Wu, Y.; Rethwisch, D.W.; Matte, A.; Cygler, M.; Linhardt, R.J.
    Role of arginine 292 in the catalytic activity of chondroitin AC lyase from Flavobacterium heparinum (2002), Biochim. Biophys. Acta, 1597, 260-270.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.5 mutant R292A, expression in Escherichia coli and conjugation to Flavobacterium heparinum to create a mutant strain Pedobacter heparinus

Protein Variants

EC Number Protein Variants Comment Organism
4.2.2.5 R292A site-directed mutagenesis, substrate recognition residue, reduced activity, action pattern is neither purely random endolytic nor purely random exolytic Pedobacter heparinus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.5 0.00286
-
hyaluronic acid wild-type enzyme, pH 8.0, 37°C Pedobacter heparinus
4.2.2.5 0.00528
-
chondroitin 6-sulfate wild-type enzyme, pH 8.0, 37°C Pedobacter heparinus
4.2.2.5 0.00544
-
chondroitin 4-sulfate wild-type enzyme, pH 8.0, 37°C Pedobacter heparinus
4.2.2.5 0.0231
-
hyaluronic acid mutant R292A, pH 8.0, 37°C Pedobacter heparinus
4.2.2.5 0.0322
-
chondroitin wild-type enzyme, pH 8.0, 37°C Pedobacter heparinus
4.2.2.5 0.0563
-
chondroitin 6-sulfate mutant R292A, pH 8.0, 37°C Pedobacter heparinus
4.2.2.5 0.0651
-
chondroitin 4-sulfate mutant R292A, pH 8.0, 37°C Pedobacter heparinus
4.2.2.5 0.119
-
chondroitin mutant R292A, pH 8.0, 37°C Pedobacter heparinus

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.5 Pedobacter heparinus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.5 recombinant mutant from mutant Flavobacterium heparinum strain Pedobacter heparinus

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.2.5 [1,3-beta-D-glucuronic acid-1,4-beta-D-hexosylamine]n = n-1 4-deoxy-beta-D-gluc-4-enuronosyl-1,4-beta-D-hexosylamine + [1,3-beta-D-glucuronic acid-1,4-beta-D-hexosylamine] acts on chondroitin 4-sulfate and chondroitin 6-sulfate, but less well on hyaluronate, reaction mechanism Pedobacter heparinus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.5 chondroitin best substrate Pedobacter heparinus ?
-
?
4.2.2.5 chondroitin sulfate A i.e. chondroitin 4-sulfate Pedobacter heparinus ?
-
?
4.2.2.5 chondroitin sulfate C i.e. chondroitin 6-sulfate Pedobacter heparinus ?
-
?
4.2.2.5 hyaluronic acid
-
Pedobacter heparinus ?
-
?
4.2.2.5 additional information the active site forms a tunnel, structurally involved residue Arg292 is responsible for substrate recognition of N-acetyl or O-sulfo moieties in galactosamine residues, it does not participate in catalysis Pedobacter heparinus ?
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.5 37
-
assay at Pedobacter heparinus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.5 8
-
assay at Pedobacter heparinus