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Literature summary extracted from

  • Masson, P.; Froment, M.T.; Fort, S.; Ribes, F.; Bec, N.; Balny, C.; Schopfer, L.M.
    Butyrylcholinesterase-catalyzed hydrolysis of N-methylindoxyl acetate: analysis of volume changes upon reaction and hysteretic behavior (2002), Biochim. Biophys. Acta, 1597, 229-243.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.8
-
 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.8 D70G catalytic activity similar to wild-type enzyme Homo sapiens
3.1.1.8 D70G/Y332A catalytic activity similar to wild-type enzyme Homo sapiens
3.1.1.8 Y332A catalytic activity similar to wild-type enzyme Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.8 0.066
-
 N-methylindoxyl acetate pH 7, 25°C, D70G mutant Homo sapiens
3.1.1.8 0.13
-
 N-methylindoxyl acetate pH 7, 25°C, Y332A mutant Homo sapiens
3.1.1.8 0.14
-
 N-methylindoxyl acetate pH 7, 25°C, wild-type Homo sapiens
3.1.1.8 0.162
-
 N-methylindoxyl acetate pH 7, 25°C, D70G/Y332A mutant Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.8 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.8 butyrylthiocholine + H2O
-
 Homo sapiens thiocholine + butyrate
-
 ?
3.1.1.8 N-methylindoxyl acetate + H2O
-
 Homo sapiens N-methylindole + acetate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.8 200
-
 N-methylindoxyl acetate pH 7, 25°C, Y332A mutant Homo sapiens
3.1.1.8 250
-
 N-methylindoxyl acetate pH 7, 25°C, D70G/Y332A mutant Homo sapiens
3.1.1.8 300
-
 N-methylindoxyl acetate pH 7, 25°C, D70G mutant Homo sapiens
3.1.1.8 300
-
 N-methylindoxyl acetate pH 7, 25°C, wild-type Homo sapiens