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Literature summary extracted from

  • Yamane, T.; Takeuchi, K.; Yamamoto, Y.; Li, Y.H.; Fujiwara, M.; Nishi, K.; Takahashi, S.; Ohkubo, I.
    Legumain from bovine kidney: its purification, molecular clonin, immunohistochemical localization and degradation of annexin II and vitamin D-binding protein (2002), Biochim. Biophys. Acta, 1596, 108-120.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.34 DNA and amino acid sequence determination and analysis Bos taurus

General Stability

EC Number General Stability Organism
3.4.22.34 2-mercaptoethanol is essentially required for stability Bos taurus
3.4.22.34 enzyme is resitant to freezing and thawing Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.34 Cd2+
-
 Bos taurus
3.4.22.34 Cu2+ strong inhibition Bos taurus
3.4.22.34 cystatin C
-
 Bos taurus
3.4.22.34 Hg2+ strong inhibition Bos taurus
3.4.22.34 High-molecular mass kininogen kinin-free, F1.2-free Bos taurus
3.4.22.34 low molecular mass kininogen
-
 Bos taurus
3.4.22.34 additional information no inhibition by E-64, leupeptin, and elastatinal Bos taurus
3.4.22.34 N-ethylmaleimide
-
 Bos taurus
3.4.22.34 p-chloromercuribenzene-sulfonic acid
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.34 0.05
-
 benzyloxycarbonyl-Ile-Ser-Asn-4-methylcoumarin-7-amide pH 5.0, 37°C Bos taurus
3.4.22.34 0.67
-
 benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin-7-amide pH 5.0, 37°C Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.34 31000
-
 gel filtration Bos taurus
3.4.22.34 34000
-
 1 * 34000, reducing SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.34 annexin II + H2O Bos taurus cleavage of the N-terminus, enzyme plays a role in inactivation and degradation of annexin II in endosomes and lysosomes ?
-
 ?
3.4.22.34 serum albumin + H2O Bos taurus
-
 ?
-
 ?
3.4.22.34 transferrin + H2O Bos taurus
-
 ?
-
 ?
3.4.22.34 vitamin D-binding protein + H2O Bos taurus enzyme is involved in the processing of mcromolecules absorbed by proximal tubule cells ?
-
 ?
3.4.22.34 ZN-alpha2-glycoprotein + H2O Bos taurus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.34 Bos taurus Q95M12
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.34 1045fold to homogeneity from kidney Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.34 kidney cortex Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.34 1.53
-
 purified enzyme Bos taurus

Storage Stability

EC Number Storage Stability Organism
3.4.22.34 4°C, 50 mM sodium citrate-buffered saline, pH 5.0, 2 mM 2-mercaptoethanol, a few weeks, stable Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.34 annexin II + H2O cleavage of the N-terminus, enzyme plays a role in inactivation and degradation of annexin II in endosomes and lysosomes Bos taurus ?
-
 ?
3.4.22.34 benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin-7-amide + H2O
-
 Bos taurus benzyloxycarbonyl-Ala-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?
3.4.22.34 benzyloxycarbonyl-Ile-Ser-Asn-4-methylcoumarin-7-amide + H2O low activity with Bos taurus benzyloxycarbonyl-Ile-Ser-Asn + 7-amino-4-methylcoumarin
-
 ?
3.4.22.34 GSVKAYTNFDAERD + H2O synthetic peptide, residues24-37, derived from the N-terminal sequence of bovine annexin II, cleavage at position N31-F32 Bos taurus GSVKAYTN + FDAERD
-
 ?
3.4.22.34 additional information substrate specificity, no activity with acetyl-Asp-Glu-Val-Asp-4-methylcoumarin-7-amide, and acetyl-Tyr-Val-Ala-Asp-4-methylcoumarin-7-amide Bos taurus ?
-
 ?
3.4.22.34 serum albumin + H2O
-
 Bos taurus ?
-
 ?
3.4.22.34 transferrin + H2O
-
 Bos taurus ?
-
 ?
3.4.22.34 vitamin D-binding protein + H2O cleavage sites are, besides others, N150-Y151, and N83-S84 Bos taurus ?
-
 ?
3.4.22.34 vitamin D-binding protein + H2O enzyme is involved in the processing of mcromolecules absorbed by proximal tubule cells Bos taurus ?
-
 ?
3.4.22.34 ZN-alpha2-glycoprotein + H2O
-
 Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.34 monomer 1 * 34000, reducing SDS-PAGE Bos taurus

Synonyms

EC Number Synonyms Comment Organism
3.4.22.34 Asparaginyl endopeptidase
-
 Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.34 37
-
 assay at Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.34 45
-
 10 min, stable up to Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.34 0.1
-
 benzyloxycarbonyl-Ile-Ser-Asn-4-methylcoumarin-7-amide pH 5.0, 37°C Bos taurus
3.4.22.34 17.9
-
 benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin-7-amide pH 5.0, 37°C Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.34 5
-
 assay at Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.34 4 6
-
 Bos taurus