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Literature summary extracted from

  • Wesolowska, O.; Krokoszynska, I.; Krowarsch, D.; Otlewski, J.
    Enhancement of chymotrypsin-inhibitor/substrate interactions by 3 M NaCl (2001), Biochim. Biophys. Acta, 1545, 78-85.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.1 Bovine pancreatic trypsin inhibitor 300fold stronger inhibition of alpha-chymotrypsin in the presence of 3 M NaCl Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.1 0.0074
-
 N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide 21°C, pH 8.3, 20 mM CaCl2 Bos taurus
3.4.21.1 0.0087
-
 N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide 21°C, pH 8.3, 20 mM CaCl2, 3 M NaCl Bos taurus
3.4.21.1 0.02
-
 N-succinyl-Ala-Ala-Pro-Met-p-nitroanilide 21°C, pH 8.3, 20 mM CaCl2, 3 M NaCl Bos taurus
3.4.21.1 0.052
-
 N-succinyl-Ala-Ala-Pro-Nle-p-nitroanilide 21°C, pH 8.3, 20 mM CaCl2, 3 M NaCl Bos taurus
3.4.21.1 0.16
-
 N-succinyl-Ala-Ala-Pro-Nva-p-nitroanilide 21°C, pH 8.3, 20 mM CaCl2, 3 M NaCl Bos taurus
3.4.21.1 0.26
-
 N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide 21°C, pH 8.3, 20 mM CaCl2 Bos taurus
3.4.21.1 0.32
-
 N-succinyl-Ala-Ala-Pro-Lys-p-nitroanilide 21°C, pH 8.3, 20 mM CaCl2, 3 M KCl Bos taurus
3.4.21.1 0.32
-
 N-succinyl-Ala-Ala-Pro-Arg-p-nitroanilide 21°C, pH 8.3, 20 mM CaCl2, 3 M NaCl Bos taurus
3.4.21.1 0.37
-
 N-succinyl-Ala-Ala-Pro-Met-p-nitroanilide 21°C, pH 8.3, 20 mM CaCl2 Bos taurus
3.4.21.1 0.44
-
 N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide 21°C, pH 8.3, 20 mM CaCl2, 3 M NaCl Bos taurus
3.4.21.1 0.49
-
 N-succinyl-Ala-Ala-Pro-Lys-p-nitroanilide 21°C, pH 8.3, 20 mM CaCl2, 800 mM NaF Bos taurus
3.4.21.1 0.51
-
 N-succinyl-Ala-Ala-Pro-Lys-p-nitroanilide 21°C, pH 8.3, 20 mM CaCl2, 3 M NaCl Bos taurus
3.4.21.1 0.58
-
 N-succinyl-Ala-Ala-Pro-Nle-p-nitroanilide 21°C, pH 8.3, 20 mM CaCl2 Bos taurus
3.4.21.1 0.61
-
 N-succinyl-Ala-Ala-Pro-Lys-p-nitroanilide 21°C, pH 8.3, 20 mM CaCl2, 3 M NaBr Bos taurus
3.4.21.1 0.92
-
 N-succinyl-Ala-Ala-Pro-Lys-p-nitroanilide 21°C, pH 8.3, 20 mM CaCl2, 3 M LiCl Bos taurus
3.4.21.1 1.6
-
 N-succinyl-Ala-Ala-Pro-Nva-p-nitroanilide 21°C, pH 8.3, 20 mM CaCl2 Bos taurus
3.4.21.1 2.6
-
 N-succinyl-Ala-Ala-Pro-Lys-p-nitroanilide 21°C, pH 8.3, 20 mM CaCl2 Bos taurus
3.4.21.1 3
-
 N-succinyl-Ala-Ala-Pro-Arg-p-nitroanilide 21°C, pH 8.3, 20 mM CaCl2 Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.1 Bos taurus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.1 pancreas
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.1 N-succinyl-Ala-Ala-Pro-Arg-p-nitroanilide + H2O very low activity Bos taurus N-succinyl-Ala-Ala-Pro-Arg + p-nitroaniline
-
 ?
3.4.21.1 N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide + H2O
-
 Bos taurus N-succinyl-Ala-Ala-Pro-Leu + p-nitroaniline
-
 ?
3.4.21.1 N-succinyl-Ala-Ala-Pro-Lys-p-nitroanilide + H2O extremly low activity Bos taurus N-succinyl-Ala-Ala-Pro-Lys + p-nitroaniline
-
 ?
3.4.21.1 N-succinyl-Ala-Ala-Pro-Met-p-nitroanilide + H2O
-
 Bos taurus N-succinyl-Ala-Ala-Pro-Met + p-nitroaniline
-
 ?
3.4.21.1 N-succinyl-Ala-Ala-Pro-Nle-p-nitroanilide + H2O
-
 Bos taurus N-succinyl-Ala-Ala-Pro-Nle + p-nitroaniline
-
 ?
3.4.21.1 N-succinyl-Ala-Ala-Pro-Nva-p-nitroanilide + H2O
-
 Bos taurus N-succinyl-Ala-Ala-Pro-Nva + p-nitroaniline
-
 ?
3.4.21.1 N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
-
 Bos taurus N-succinyl-Ala-Ala-Pro-Phe + p-nitroaniline
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.1 0.006
-
 N-succinyl-Ala-Ala-Pro-Lys-p-nitroanilide 21°C, pH 8.3, 20 mM CaCl2 Bos taurus
3.4.21.1 0.01
-
 N-succinyl-Ala-Ala-Pro-Lys-p-nitroanilide 21°C, pH 8.3, 20 mM CaCl2, 3 M LiCl Bos taurus
3.4.21.1 0.014
-
 N-succinyl-Ala-Ala-Pro-Arg-p-nitroanilide 21°C, pH 8.3, 20 mM CaCl2 Bos taurus
3.4.21.1 0.026
-
 N-succinyl-Ala-Ala-Pro-Lys-p-nitroanilide 21°C, pH 8.3, 20 mM CaCl2, 3 M KCl Bos taurus
3.4.21.1 0.031 0.51 N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide 21°C, pH 8.3, 20 mM CaCl2 Bos taurus
3.4.21.1 0.04 1.97 N-succinyl-Ala-Ala-Pro-Nva-p-nitroanilide 21°C, pH 8.3, 20 mM CaCl2, 3 M NaCl Bos taurus
3.4.21.1 0.05
-
 N-succinyl-Ala-Ala-Pro-Arg-p-nitroanilide 21°C, pH 8.3, 20 mM CaCl2, 3 M NaCl Bos taurus
3.4.21.1 0.26
-
 N-succinyl-Ala-Ala-Pro-Lys-p-nitroanilide 21°C, pH 8.3, 20 mM CaCl2, 3 M NaCl Bos taurus
3.4.21.1 0.27
-
 N-succinyl-Ala-Ala-Pro-Lys-p-nitroanilide 21°C, pH 8.3, 20 mM CaCl2, 800 mM NaF Bos taurus
3.4.21.1 0.34
-
 N-succinyl-Ala-Ala-Pro-Lys-p-nitroanilide 21°C, pH 8.3, 20 mM CaCl2, 3 M NaBr Bos taurus
3.4.21.1 1.45
-
 N-succinyl-Ala-Ala-Pro-Met-p-nitroanilide 21°C, pH 8.3, 20 mM CaCl2 Bos taurus
3.4.21.1 3.3
-
 N-succinyl-Ala-Ala-Pro-Nva-p-nitroanilide 21°C, pH 8.3, 20 mM CaCl2 Bos taurus
3.4.21.1 5.32
-
 N-succinyl-Ala-Ala-Pro-Nva-p-nitroanilide 21°C, pH 8.3, 20 mM CaCl2 Bos taurus
3.4.21.1 8.04
-
 N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide 21°C, pH 8.3, 20 mM CaCl2 Bos taurus
3.4.21.1 9.31
-
 N-succinyl-Ala-Ala-Pro-Nva-p-nitroanilide 21°C, pH 8.3, 20 mM CaCl2, 3 M NaCl Bos taurus
3.4.21.1 12
-
 N-succinyl-Ala-Ala-Pro-Nle-p-nitroanilide 21°C, pH 8.3, 20 mM CaCl2 Bos taurus
3.4.21.1 15
-
 N-succinyl-Ala-Ala-Pro-Met-p-nitroanilide 21°C, pH 8.3, 20 mM CaCl2, 3 M NaCl Bos taurus
3.4.21.1 16
-
 N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide 21°C, pH 8.3, 20 mM CaCl2, 3 M NaCl Bos taurus
3.4.21.1 18
-
 N-succinyl-Ala-Ala-Pro-Nle-p-nitroanilide 21°C, pH 8.3, 20 mM CaCl2, 3 M NaCl Bos taurus
3.4.21.1 77
-
 N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide 21°C, pH 8.3, 20 mM CaCl2, 3 M NaCl Bos taurus
3.4.21.1 110
-
 N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide 21°C, pH 8.3, 20 mM CaCl2 Bos taurus