Any feedback?
Literature summary extracted from
- Protein engineering of subtilisin (2000), Biochim. Biophys. Acta, 1543, 203-222.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.4.21.62 | analysis | model for understanding the enormous rate enhancements affected by enzymes | Bacillus sp. (in: firmicutes) |
| 3.4.21.62 | synthesis | C221 subtilisin is catalytically wounded to the point that will barely hydrolyze peptide bonds but turn to be quite reactive with certain activated ester substrates, therefore a useful tool for catalyzing synthetic reactions | Bacillus sp. (in: firmicutes) |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.21.62 | - |
Bacillus sp. (in: firmicutes) |
| 3.4.21.62 | subtilisin BPN' | Bacillus amyloliquefaciens |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.4.21.62 | - |
Bacillus sp. (in: firmicutes) |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.4.21.62 | A116E | site-directed mutagenesis, BPN' | Bacillus amyloliquefaciens |
| 3.4.21.62 | A194P | site-directed mutagenesis, savinase | Bacillus amyloliquefaciens |
| 3.4.21.62 | D97G | site-directed mutagenesis, subtilisin E | Bacillus amyloliquefaciens |
| 3.4.21.62 | E89S | site-directed mutagenesis, BPN' | Bacillus amyloliquefaciens |
| 3.4.21.62 | G131D | site-directed mutagenesis, BPN' | Bacillus amyloliquefaciens |
| 3.4.21.62 | G166R | site-directed mutagenesis, subtilisin E | Bacillus amyloliquefaciens |
| 3.4.21.62 | G166S | site-directed mutagenesis, BPN' | Bacillus amyloliquefaciens |
| 3.4.21.62 | G169A | site-directed mutagenesis, BPN' | Bacillus amyloliquefaciens |
| 3.4.21.62 | G195E | site-directed mutagenesis, BPN' | Bacillus amyloliquefaciens |
| 3.4.21.62 | H120D | site-directed mutagenesis, savinase | Bacillus amyloliquefaciens |
| 3.4.21.62 | I107V | site-directed mutagenesis, BPN' | Bacillus amyloliquefaciens |
| 3.4.21.62 | K213R | site-directed mutagenesis, BPN' | Bacillus amyloliquefaciens |
| 3.4.21.62 | K235L | site-directed mutagenesis, savinase | Bacillus amyloliquefaciens |
| 3.4.21.62 | K256Y | site-directed mutagenesis, BPN' | Bacillus amyloliquefaciens |
| 3.4.21.62 | K27R | site-directed mutagenesis, BPN' | Bacillus amyloliquefaciens |
| 3.4.21.62 | L126I | site-directed mutagenesis, BPN' | Bacillus amyloliquefaciens |
| 3.4.21.62 | M50F | site-directed mutagenesis, BPN' | Bacillus amyloliquefaciens |
| 3.4.21.62 | N109S | site-directed mutagenesis, BPN' | Bacillus amyloliquefaciens |
| 3.4.21.62 | N118S | site-directed mutagenesis, subtilisin E | Bacillus amyloliquefaciens |
| 3.4.21.62 | N181D | site-directed mutagenesis, subtilisin E | Bacillus amyloliquefaciens |
| 3.4.21.62 | N218D | site-directed mutagenesis, BPN' | Bacillus amyloliquefaciens |
| 3.4.21.62 | N218S | site-directed mutagenesis, subtilisin E | Bacillus amyloliquefaciens |
| 3.4.21.62 | N218S | site-directed mutagenesis, BPN' | Bacillus amyloliquefaciens |
| 3.4.21.62 | N76D | site-directed mutagenesis, subtilisin E | Bacillus amyloliquefaciens |
| 3.4.21.62 | N76D | site-directed mutagenesis, BPN' | Bacillus amyloliquefaciens |
| 3.4.21.62 | N77K | site-directed mutagenesis, BPN' | Bacillus amyloliquefaciens |
| 3.4.21.62 | P14L | site-directed mutagenesis, subtilisin E | Bacillus amyloliquefaciens |
| 3.4.21.62 | P172D | site-directed mutagenesis, BPN' | Bacillus amyloliquefaciens |
| 3.4.21.62 | Q19E/Q271E | site-directed mutagenesis, BPN' | Bacillus amyloliquefaciens |
| 3.4.21.62 | Q206Cox | site-directed mutagenesis, BPN' | Bacillus amyloliquefaciens |
| 3.4.21.62 | Q271E | site-directed mutagenesis, BPN' | Bacillus amyloliquefaciens |
| 3.4.21.62 | R170Y | site-directed mutagenesis, BPN' | Bacillus amyloliquefaciens |
| 3.4.21.62 | S128G | site-directed mutagenesis, savinase | Bacillus amyloliquefaciens |
| 3.4.21.62 | S161C | site-directed mutagenesis, subtilisin E | Bacillus amyloliquefaciens |
| 3.4.21.62 | S188P | site-directed mutagenesis, BPN' | Bacillus amyloliquefaciens |
| 3.4.21.62 | S194P | site-directed mutagenesis, subtilisin E | Bacillus amyloliquefaciens |
| 3.4.21.62 | S194P | site-directed mutagenesis, BPN' | Bacillus amyloliquefaciens |
| 3.4.21.62 | S221C | site-directed mutagenesis, BPN' | Bacillus amyloliquefaciens |
| 3.4.21.62 | S53TI | site-directed mutagenesis, BPN' | Bacillus amyloliquefaciens |
| 3.4.21.62 | S63D | site-directed mutagenesis, BPN' | Bacillus amyloliquefaciens |
| 3.4.21.62 | S78D | site-directed mutagenesis, BPN' | Bacillus amyloliquefaciens |
| 3.4.21.62 | T254A | site-directed mutagenesis, BPN' | Bacillus amyloliquefaciens |
| 3.4.21.62 | V205I | site-directed mutagenesis, savinase | Bacillus amyloliquefaciens |
| 3.4.21.62 | V8I | site-directed mutagenesis, BPN' | Bacillus amyloliquefaciens |
| 3.4.21.62 | Y217K | site-directed mutagenesis, BPN' | Bacillus amyloliquefaciens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.62 | EDTA | - |
Bacillus amyloliquefaciens |  |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.62 | additional information | Bacillus sp. (in: firmicutes) | biosynthesis of subtilisin requires participation of an N-terminal prodomain | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.62 | Bacillus amyloliquefaciens | - |
- |
- |
| 3.4.21.62 | Bacillus sp. (in: firmicutes) | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.21.62 | - |
Bacillus sp. (in: firmicutes) |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.62 | additional information | biosynthesis of subtilisin requires participation of an N-terminal prodomain | Bacillus sp. (in: firmicutes) | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.21.62 | BPN' | - |
Bacillus amyloliquefaciens |
| 3.4.21.62 | subtilisin S88 | - |
Bacillus amyloliquefaciens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2026.1 (March 2026)
Legal
Curated at
Member of
![DSMZ Digital Diversity]()
![Global Core Biodata Resource]()
![ELIXIR Core Data Resource]()
![German Network for Bioinformatics Infrastructure]()
