BRENDA - Enzyme Database

Marked activation of the N-acylphosphatidylethanolamine-hydrolyzing phosphodiesterase by divalent cations

Ueda, N.; Liu, Q.; Yamanaka, K.; Biochim. Biophys. Acta 1532, 121-127 (2001)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.4
Triton X-100
up to 0.4%, inhibitory above
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.4
Cu2+
10 mM, complete inhibition
Rattus norvegicus
3.1.4.4
Fe2+
10 mM, complete inhibition
Rattus norvegicus
3.1.4.4
Hg2+
10 mM, complete inhibition
Rattus norvegicus
3.1.4.4
Triton X-100
activating up to 0.4%, inhibitory above
Rattus norvegicus
3.1.4.4
Zn2+
10 mM, complete inhibition
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.4
0.008
-
N-palmitoyl-phosphatidylethanolamine
pH 7.5, 37°C, in presence of Cacl2
Rattus norvegicus
3.1.4.4
0.045
-
N-palmitoyl-phosphatidylethanolamine
pH 7.5, 37°C, in presence of Triton X-100
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.4
particle-bound
-
Rattus norvegicus
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.4
Ba2+
marked stimulation
Rattus norvegicus
3.1.4.4
Ca2+
10 mM, up to 30-fold activation
Rattus norvegicus
3.1.4.4
Co2+
marked stimulation
Rattus norvegicus
3.1.4.4
Mg2+
marked stimulation
Rattus norvegicus
3.1.4.4
Mn2+
marked stimulation
Rattus norvegicus
3.1.4.4
Ni2+
marked stimulation
Rattus norvegicus
3.1.4.4
Sr2+
marked stimulation
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.4
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.4.4
partial
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.4
heart
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.4
17000
-
pH 7.5, 37°C
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
N-arachidonoyl-phosphatidylethanolamine + H2O
-
650338
Rattus norvegicus
phosphatidic acid + N-arachidonoylethanolamine
-
650338
Rattus norvegicus
?
3.1.4.4
N-palmitoyl-phosphatidylethanolamine + H2O
-
650338
Rattus norvegicus
phosphatidic acid + N-palmitoylethanolamine
-
650338
Rattus norvegicus
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.4.4
90
-
10 min, complete loss of activity
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.4
7.5
-
-
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.4
Triton X-100
up to 0.4%, inhibitory above
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.4
Cu2+
10 mM, complete inhibition
Rattus norvegicus
3.1.4.4
Fe2+
10 mM, complete inhibition
Rattus norvegicus
3.1.4.4
Hg2+
10 mM, complete inhibition
Rattus norvegicus
3.1.4.4
Triton X-100
activating up to 0.4%, inhibitory above
Rattus norvegicus
3.1.4.4
Zn2+
10 mM, complete inhibition
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.4
0.008
-
N-palmitoyl-phosphatidylethanolamine
pH 7.5, 37°C, in presence of Cacl2
Rattus norvegicus
3.1.4.4
0.045
-
N-palmitoyl-phosphatidylethanolamine
pH 7.5, 37°C, in presence of Triton X-100
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.4
particle-bound
-
Rattus norvegicus
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.4
Ba2+
marked stimulation
Rattus norvegicus
3.1.4.4
Ca2+
10 mM, up to 30-fold activation
Rattus norvegicus
3.1.4.4
Co2+
marked stimulation
Rattus norvegicus
3.1.4.4
Mg2+
marked stimulation
Rattus norvegicus
3.1.4.4
Mn2+
marked stimulation
Rattus norvegicus
3.1.4.4
Ni2+
marked stimulation
Rattus norvegicus
3.1.4.4
Sr2+
marked stimulation
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.4
partial
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.4
heart
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.4
17000
-
pH 7.5, 37°C
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
N-arachidonoyl-phosphatidylethanolamine + H2O
-
650338
Rattus norvegicus
phosphatidic acid + N-arachidonoylethanolamine
-
650338
Rattus norvegicus
?
3.1.4.4
N-palmitoyl-phosphatidylethanolamine + H2O
-
650338
Rattus norvegicus
phosphatidic acid + N-palmitoylethanolamine
-
650338
Rattus norvegicus
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.4.4
90
-
10 min, complete loss of activity
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.4
7.5
-
-
Rattus norvegicus