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Literature summary extracted from

  • Rezaie, A.R.; Yang, L.
    Probing the molecular basis of factor Xa specificity by mutagenesis of the serpin, antithrombin (2001), Biochim. Biophys. Acta, 1528, 167-176.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.6 antithrombin i.e. HAT, serpin, human, recombinant from expression in human 293 cells, inhibition is increased by heparin Homo sapiens
3.4.21.6 antithrombin mutant HAT/Proth-1 mutant of human antithrombin possessing factor Xa-cleavage site 1 from human thrombin, recombinant from expression in inhuman 293 cells, inactivation of factor Xa is 3-4fold more effective that with mutant HAT/Proth-2, increased inactivation by heparin and 300-500fold by pentasaccharide fragment of heparin Homo sapiens
3.4.21.6 antithrombin mutant HAT/Proth-2 mutant of human antithrombin possessing factor Xa-cleavage site 2 from human thrombin, recombinant from expression in inhuman 293 cells, increased inactivation by heparin and 300-500fold by pentasaccharide fragment of heparin Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.6 0.00023
-
 prothrombin prothrombinase complex, pH 7.4, 22°C Homo sapiens
3.4.21.6 0.0088
-
 prethrombin-2 prothrombinase complex, pH 7.4, 22°C Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.6 prothrombin + H2O Homo sapiens
-
 thrombin + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.6 Homo sapiens
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.21.6 selective cleavage of Arg-/-Thr and then Arg-/-Ile bonds in prothrombin to form thrombin scutelarin has similar specificity, catalytic mechanism Homo sapiens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.6 prethrombin-2 + H2O smallest zymogen form of thrombin containing only the factor Xa cleavage site 2 Homo sapiens thrombin-2
-
 ?
3.4.21.6 prothrombin + H2O
-
 Homo sapiens thrombin + ?
-
 ?
3.4.21.6 prothrombin + H2O the cleavage site 2 is preferred to cleavage site 1 Homo sapiens thrombin + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.6 More Ca2+, factor Xa and factor Va are part of the prothrombinase enzyme complex Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.4.21.6 factor Xa
-
 Homo sapiens
3.4.21.6 More the enzyme belongs to the peptidase family S1, i.e. trypsin family Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.6 22
-
 room temperature, assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.6 19.6
-
 prothrombin prothrombinase complex, pH 7.4, 22°C Homo sapiens
3.4.21.6 20.5
-
 prethrombin-2 prothrombinase complex, pH 7.4, 22°C Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.6 7.4
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
3.4.21.6 Factor Va part of the prothrombinase enzyme complex Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.21.6 additional information
-
 additional information inactivation kinetics of wild-type and mutant antithrombins Homo sapiens