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Literature summary extracted from

  • Mio, T.; Yamada-Okabe, T.; Arisawa, M.; Yamada-Okabe, H.
    Functional cloning and mutational analysis of the human cDNA for phosphoacetylglucosamine mutase: identification of the amino acid residues essential for the catalysis (2000), Biochim. Biophys. Acta, 1492, 369-376.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.2.3 expression in Escherichia coli Homo sapiens
5.4.2.3 expression in Escherichia coli Candida albicans

Protein Variants

EC Number Protein Variants Comment Organism
5.4.2.3 D276A no activity Homo sapiens
5.4.2.3 D276E extremely low activity Homo sapiens
5.4.2.3 D278A no activity Homo sapiens
5.4.2.3 D278E no activity Homo sapiens
5.4.2.3 D280A extremely low activity Homo sapiens
5.4.2.3 D70A 65% of wild type activity Homo sapiens
5.4.2.3 G277A 79% of wild type activity Homo sapiens
5.4.2.3 G499A 0.5% of wild type activity Homo sapiens
5.4.2.3 G58A 20% of wild type activity Homo sapiens
5.4.2.3 G72A 3% of wild type activity Homo sapiens
5.4.2.3 H65A no activity Homo sapiens
5.4.2.3 H65R very low activity Homo sapiens
5.4.2.3 K74A no activity Homo sapiens
5.4.2.3 N66A 1.3% of wild type activity Homo sapiens
5.4.2.3 P67A 100% of wild type activity Homo sapiens
5.4.2.3 R281A no activity Homo sapiens
5.4.2.3 R281K no activity Homo sapiens
5.4.2.3 R496A extremely low activity Homo sapiens
5.4.2.3 R498A 8% of wild type activity Homo sapiens
5.4.2.3 S64A no activity Homo sapiens
5.4.2.3 S64T no activity Homo sapiens
5.4.2.3 T500A extremely low activity Homo sapiens
5.4.2.3 T62A 106% of wild type activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.2.3 0.046
-
N-acetyl-alpha-D-glucosamine
-
Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.2.3 N-acetyl-alpha-D-glucosamine 1-phosphate Homo sapiens
-
N-acetyl-D-glucosamine 6-phosphate
-
?
5.4.2.3 N-acetyl-alpha-D-glucosamine 1-phosphate Candida albicans
-
N-acetyl-D-glucosamine 6-phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
5.4.2.3 Candida albicans Q9P4V2
-
-
5.4.2.3 Homo sapiens O95394
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.2.3 recombinant CaAgm1-GST fusion protein Candida albicans
5.4.2.3 recombinant HsAgm1-GST fusion protein Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.2.3 N-acetyl-alpha-D-glucosamine
-
Homo sapiens ?
-
?
5.4.2.3 N-acetyl-alpha-D-glucosamine 1-phosphate
-
Homo sapiens N-acetyl-D-glucosamine 6-phosphate
-
?
5.4.2.3 N-acetyl-alpha-D-glucosamine 1-phosphate
-
Candida albicans N-acetyl-D-glucosamine 6-phosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
5.4.2.3 CaAGM1
-
Candida albicans
5.4.2.3 HsAGM1
-
Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.4.2.3 1.79
-
N-acetyl-alpha-D-glucosamine
-
Homo sapiens
5.4.2.3 2.94
-
N-acetyl-alpha-D-glucosamine
-
Homo sapiens