EC Number | Cloned (Comment) | Organism |
---|---|---|
6.1.1.4 | mitochondrial isozyme, DNA sequence determination and analysis, expression of the mature enzyme as His-tagged enzyme in Escherichia coli BL21(DE3) | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.4 | additional information | inhibition by high levels of mono- and divalent cations | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.4 | 0.014 | - |
tRNALeu | recombinant mitochondrial isozyme, pH 7.6, 37°C | Homo sapiens | |
6.1.1.4 | 0.045 | - |
L-leucine | recombinant mitochondrial isozyme, pH 7.6, 37°C | Homo sapiens | |
6.1.1.4 | 0.09 | - |
ATP | recombinant mitochondrial isozyme, pH 7.6, 37°C | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
6.1.1.4 | mitochondrion | mitochondrial isozyme | Homo sapiens | 5739 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.1.1.4 | 101000 | - |
recombinant enzyme, gel filtration | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.4 | ATP + L-leucine + tRNALeu | Homo sapiens | - |
AMP + diphosphate + L-leucyl-tRNALeu | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.1.1.4 | Homo sapiens | Q15031 | class I enzyme | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.4 | ATP + L-leucine + tRNALeu | - |
Homo sapiens | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
6.1.1.4 | ATP + L-leucine + tRNALeu | tRNALeu substrate from Escherichia coli, 2-step reaction, the first step is reversible, while the second step is not | Homo sapiens | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
6.1.1.4 | additional information | enzyme also performs the ATP-diphosphate exchange reaction | Homo sapiens | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.1.1.4 | monomer | 1 * 101000, recombinant enzyme, SDS-PAGE | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.1.1.4 | Leucine translase | - |
Homo sapiens |
6.1.1.4 | Leucine--tRNA ligase | - |
Homo sapiens |
6.1.1.4 | Leucyl-transfer ribonucleate synthetase | - |
Homo sapiens |
6.1.1.4 | Leucyl-transfer ribonucleic acid synthetase | - |
Homo sapiens |
6.1.1.4 | Leucyl-transfer RNA synthetase | - |
Homo sapiens |
6.1.1.4 | Leucyl-tRNA synthetase | - |
Homo sapiens |
6.1.1.4 | LeuRS | - |
Homo sapiens |
6.1.1.4 | More | enzyme belongs to the XUX aminoacyl synthetase family | Homo sapiens |
6.1.1.4 | mtLeuRS | - |
Homo sapiens |
6.1.1.4 | Synthetase, leucyl-transfer ribonucleate | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.1.1.4 | 37 | - |
assay at | Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.4 | 0.12 | - |
tRNALeu | recombinant mitochondrial isozyme, pH 7.6, 37°C | Homo sapiens | |
6.1.1.4 | 0.8 | - |
ATP | recombinant mitochondrial isozyme, pH 7.6, 37°C | Homo sapiens | |
6.1.1.4 | 2.7 | - |
L-leucine | recombinant mitochondrial isozyme, pH 7.6, 37°C | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.1.1.4 | 7.6 | - |
assay at | Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.4 | ATP | - |
Homo sapiens |