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Literature summary extracted from

  • Bullard, J.M.; Cai, Y.C.; Spremulli, L.L.
    Expression and characterization of the human mitochondrial leucyl-tRNA synthetase (2000), Biochim. Biophys. Acta, 1490, 245-258.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.4 mitochondrial isozyme, DNA sequence determination and analysis, expression of the mature enzyme as His-tagged enzyme in Escherichia coli BL21(DE3) Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.4 additional information inhibition by high levels of mono- and divalent cations Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.4 0.014
-
tRNALeu recombinant mitochondrial isozyme, pH 7.6, 37°C Homo sapiens
6.1.1.4 0.045
-
L-leucine recombinant mitochondrial isozyme, pH 7.6, 37°C Homo sapiens
6.1.1.4 0.09
-
ATP recombinant mitochondrial isozyme, pH 7.6, 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.4 mitochondrion mitochondrial isozyme Homo sapiens 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.4 101000
-
recombinant enzyme, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.4 ATP + L-leucine + tRNALeu Homo sapiens
-
AMP + diphosphate + L-leucyl-tRNALeu
-
?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.4 Homo sapiens Q15031 class I enzyme
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.4 ATP + L-leucine + tRNALeu
-
Homo sapiens AMP + diphosphate + L-leucyl-tRNALeu
-
?
6.1.1.4 ATP + L-leucine + tRNALeu tRNALeu substrate from Escherichia coli, 2-step reaction, the first step is reversible, while the second step is not Homo sapiens AMP + diphosphate + L-leucyl-tRNALeu
-
?
6.1.1.4 additional information enzyme also performs the ATP-diphosphate exchange reaction Homo sapiens ?
-
?

Subunits

EC Number Subunits Comment Organism
6.1.1.4 monomer 1 * 101000, recombinant enzyme, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
6.1.1.4 Leucine translase
-
Homo sapiens
6.1.1.4 Leucine--tRNA ligase
-
Homo sapiens
6.1.1.4 Leucyl-transfer ribonucleate synthetase
-
Homo sapiens
6.1.1.4 Leucyl-transfer ribonucleic acid synthetase
-
Homo sapiens
6.1.1.4 Leucyl-transfer RNA synthetase
-
Homo sapiens
6.1.1.4 Leucyl-tRNA synthetase
-
Homo sapiens
6.1.1.4 LeuRS
-
Homo sapiens
6.1.1.4 More enzyme belongs to the XUX aminoacyl synthetase family Homo sapiens
6.1.1.4 mtLeuRS
-
Homo sapiens
6.1.1.4 Synthetase, leucyl-transfer ribonucleate
-
Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.4 37
-
assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.4 0.12
-
tRNALeu recombinant mitochondrial isozyme, pH 7.6, 37°C Homo sapiens
6.1.1.4 0.8
-
ATP recombinant mitochondrial isozyme, pH 7.6, 37°C Homo sapiens
6.1.1.4 2.7
-
L-leucine recombinant mitochondrial isozyme, pH 7.6, 37°C Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.4 7.6
-
assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.4 ATP
-
Homo sapiens