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Literature summary extracted from

  • Watt, D.K.; Ono, H.; Hayashi, K.
    Agrobacterium tumefaciens beta-glucosidase is also an effective beta-xylosidase, and has a high transglycosylation activity in the presence of alcohols (1998), Biochim. Biophys. Acta, 1385, 78-88.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.126 1-butanol 20 mM, activates due to transglycosylation activity with over 80% of p-nitrophenyl beta-D-glucopyranoside being converted to 1-butyl beta-D-glucopyranoside in the presence of Cbg1 and 100 mM 1-butanol Agrobacterium tumefaciens
3.2.1.126 1-Heptanol 20 mM, activates Agrobacterium tumefaciens
3.2.1.126 1-Hexanol 20 mM, activates Agrobacterium tumefaciens
3.2.1.126 1-Octanol 20 mM, activates Agrobacterium tumefaciens
3.2.1.126 1-Pentanol 20 mM, activates Agrobacterium tumefaciens
3.2.1.126 1-propanol 20 mM, activates, highest rate of consumption of p-nitrophenyl beta-D-glucopyranoside in the presence of 0.6 M 1-propanol Agrobacterium tumefaciens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.126 expression in Escherichia coli Agrobacterium tumefaciens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.126 D-glucose competitive inhibitor, p-nitrophenyl beta-D-glucopyranoside as substrate Agrobacterium tumefaciens
3.2.1.126 deoxynojirimycin strong, competitive inhibitor, p-nitrophenyl beta-D-glucopyranoside as substrate Agrobacterium tumefaciens
3.2.1.126 glucuronolactone strong, competitive inhibitor, p-nitrophenyl beta-D-glucopyranoside as substrate Agrobacterium tumefaciens
3.2.1.126 isopropyl 1-thio-beta-D-glucopyranoside competitive inhibitor, p-nitrophenyl beta-D-glucopyranoside as substrate Agrobacterium tumefaciens
3.2.1.126 additional information not inhibited by 24 mM xylose Agrobacterium tumefaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.126 additional information
-
 additional information
-
 Agrobacterium tumefaciens
3.2.1.126 0.005
-
 p-nitrophenyl beta-D-xylopyranoside pH 6.5, 30°C Agrobacterium tumefaciens
3.2.1.126 0.012
-
 p-nitrophenyl beta-D-glucopyranoside pH 6.5, 30°C Agrobacterium tumefaciens
3.2.1.126 0.021
-
 arbutin pH 6.5, 30°C Agrobacterium tumefaciens
3.2.1.126 0.023
-
 coniferin pH 6.5, 30°C Agrobacterium tumefaciens
3.2.1.126 0.045
-
 Salicin pH 6.5, 30°C Agrobacterium tumefaciens
3.2.1.126 0.06
-
 o-nitrophenyl beta-D-glucopyranoside pH 6.5, 30°C Agrobacterium tumefaciens
3.2.1.126 0.08
-
 p-nitrophenyl beta-D-fucopyranoside pH 6.5, 30°C Agrobacterium tumefaciens
3.2.1.126 0.27
-
 p-nitrophenyl alpha-L-arabinofuranoside pH 6.5, 30°C Agrobacterium tumefaciens
3.2.1.126 4
-
 p-nitrophenyl beta-D-galactopyranoside pH 6.5, 30°C Agrobacterium tumefaciens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.126 88289
-
 x * 98000, about, SDS-PAGE, x * 88289, sequence calculation Agrobacterium tumefaciens
3.2.1.126 98000
-
 x * 98000, about, SDS-PAGE, x * 88289, sequence calculation Agrobacterium tumefaciens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.126 coniferin + H2O Agrobacterium tumefaciens natural substrate D-glucose + coniferol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.126 Agrobacterium tumefaciens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.126 recombinant Cbg1 Agrobacterium tumefaciens

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.126 coniferin + H2O = D-glucose + coniferol mechanism Agrobacterium tumefaciens
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.126 39 41 pH 6.5, 30°C Agrobacterium tumefaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.126 arbutin + H2O
-
 Agrobacterium tumefaciens ?
-
 ?
3.2.1.126 coniferin + H2O natural substrate Agrobacterium tumefaciens D-glucose + coniferol
-
 ?
3.2.1.126 coniferin + H2O 20000fold preference for the natural substrate coniferin over cellobiose Agrobacterium tumefaciens D-glucose + coniferol
-
 ?
3.2.1.126 additional information enzyme has a high transglycosylation activity in the presence of alcohols, transglycosylation mechanism Agrobacterium tumefaciens ?
-
 ?
3.2.1.126 o-nitrophenyl beta-D-glucopyranoside + H2O
-
 Agrobacterium tumefaciens beta-D-glucose + o-nitrophenol
-
 ?
3.2.1.126 p-nitrophenyl alpha-L-arabinofuranoside + H2O with moderate efficiency Agrobacterium tumefaciens p-nitrophenol + alpha-L-arabinofuranose
-
 ?
3.2.1.126 p-nitrophenyl beta-cellobioside + H2O releases only terminal glucose from the substrate Agrobacterium tumefaciens ?
-
 ?
3.2.1.126 p-nitrophenyl beta-D-fucopyranoside + H2O with moderate efficiency Agrobacterium tumefaciens beta-D-fucose + p-nitrophenol
-
 ?
3.2.1.126 p-nitrophenyl beta-D-galactopyranoside + H2O with low efficiency Agrobacterium tumefaciens beta-D-galactose + p-nitrophenol
-
 ?
3.2.1.126 p-nitrophenyl beta-D-glucopyranoside + H2O
-
 Agrobacterium tumefaciens beta-D-glucose + p-nitrophenol
-
 ?
3.2.1.126 p-nitrophenyl beta-D-xylopyranoside + H2O 73% of the efficiency with p-nitrophenyl beta-D-glucopyranoside Agrobacterium tumefaciens beta-D-xylose + p-nitrophenol
-
 ?
3.2.1.126 salicin + H2O
-
 Agrobacterium tumefaciens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.126 ? x * 98000, about, SDS-PAGE, x * 88289, sequence calculation Agrobacterium tumefaciens

Synonyms

EC Number Synonyms Comment Organism
3.2.1.126 Cbg1
-
 Agrobacterium tumefaciens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.126 30
-
 assay at Agrobacterium tumefaciens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.126 additional information
-
 additional information
-
 Agrobacterium tumefaciens
3.2.1.126 3.15
-
 Salicin pH 6.5, 30°C Agrobacterium tumefaciens
3.2.1.126 3.2
-
 p-nitrophenyl beta-D-glucopyranoside pH 6.5, 30°C Agrobacterium tumefaciens
3.2.1.126 22.1
-
 p-nitrophenyl beta-D-fucopyranoside pH 6.5, 30°C Agrobacterium tumefaciens
3.2.1.126 28.9
-
 p-nitrophenyl beta-D-xylopyranoside pH 6.5, 30°C Agrobacterium tumefaciens
3.2.1.126 46.6
-
 p-nitrophenyl beta-D-galactopyranoside pH 6.5, 30°C Agrobacterium tumefaciens
3.2.1.126 86.7
-
 Salicin pH 6.5, 30°C Agrobacterium tumefaciens
3.2.1.126 95.4
-
 p-nitrophenyl beta-D-glucopyranoside pH 6.5, 30°C Agrobacterium tumefaciens
3.2.1.126 106
-
 coniferin pH 6.5, 30°C Agrobacterium tumefaciens
3.2.1.126 118
-
 p-nitrophenyl alpha-L-arabinofuranoside pH 6.5, 30°C Agrobacterium tumefaciens
3.2.1.126 155
-
 o-nitrophenyl beta-D-glucopyranoside pH 6.5, 30°C Agrobacterium tumefaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.126 6.5
-
 assay at Agrobacterium tumefaciens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.126 0.00078
-
 deoxynojirimycin pH 6.5, 30°C Agrobacterium tumefaciens
3.2.1.126 0.0039
-
 glucuronolactone pH 6.5, 30°C Agrobacterium tumefaciens
3.2.1.126 2.9
-
 D-glucose pH 6.5, 30°C Agrobacterium tumefaciens
3.2.1.126 5.6
-
 isopropyl 1-thio-beta-D-glucopyranoside pH 6.5, 30°C Agrobacterium tumefaciens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.126 Agrobacterium tumefaciens isoelectric focusing, pH gradient 4-7
-
 4.5