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Literature summary extracted from

  • Zhukov, A.; Hellman, U.; Ingelman-Sundberg, M.
    Purification and characterization of hepsin from rat liver microsomes (1997), Biochim. Biophys. Acta, 1337, 85-95.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.106 pepstatin A 21% activation at 0.1 mM Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.106 3,4-dichloroisocoumarin 94% inhibition at 0.1 mM Rattus norvegicus
3.4.21.106 diisopropylfluorophosphate complete inhibition at 5 mM Rattus norvegicus
3.4.21.106 leupeptin 99% inhibition at 0.1 mM Rattus norvegicus
3.4.21.106 phenylmethanesulfonyl fluoride 80% inhibition at 1 mM Rattus norvegicus
3.4.21.106 ZnSO4 26% inhibition at 1 mM Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.106 membrane
-
 Rattus norvegicus 16020
-
3.4.21.106 microsome
-
 Rattus norvegicus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.106 19000
-
 1 * 31000 + 1 * 19000, SDS-PAGE under reducing conditions Rattus norvegicus
3.4.21.106 31000
-
 1 * 31000 + 1 * 19000, SDS-PAGE under reducing conditions Rattus norvegicus
3.4.21.106 50000
-
 zymogen Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.106 Protein + H2O Rattus norvegicus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.106 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.106 proteolytic modification zymogen is converted to the active protease by cleavage between Arg161 and Ile162 Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.106 homogeneity Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.106 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.106 10.67
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.106 additional information cleaves after basic amino acid residues, Arg being preferable to Lys Rattus norvegicus ?
-
 ?
3.4.21.106 N-benzyloxycarbonyl-Ala-Arg-Arg 4-methylcoumarin 7-amide + H2O weak substrate Rattus norvegicus N-benzyloxycarbonyl-Ala-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.106 N-tert-butyloxycarbonyl-Gly-Lys-Arg 4-methylcoumarin 7-amide + H2O weak substrate Rattus norvegicus N-tert-butyloxycarbonyl-Gly-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.106 Protein + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.21.106 tert-butyloxycarbonyl-Gln-Ala-Arg 4-methylcoumarin 7-amide + H2O weak substrate Rattus norvegicus tert-butyloxycarbonyl-Gln-Ala-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.106 tert-butyloxycarbonyl-Gln-Arg-Arg 4-methylcoumarin 7-amide + H2O
-
 Rattus norvegicus tert-butyloxycarbonyl-Gln-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.106 tert-Butyloxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide + H2O weak substrate Rattus norvegicus tert-Butyloxycarbonyl-Val-Pro-Arg + 7-amino-4-methylcoumarin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.106 dimer 1 * 31000 + 1 * 19000, SDS-PAGE under reducing conditions Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
3.4.21.106 S01.224 Merops ID Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.106 6 9 activity with in the range pH 6-9 has no maximum, the activity increases continuously Rattus norvegicus