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Literature summary extracted from

  • Ligne, T.; Pauthe, E.; Monti, J.P.; Gacel, G.; Larreta-Garde, V.
    Additional data about thermolysin specificity in buffer- and glycerol-containing media (1997), Biochim. Biophys. Acta, 1337, 143-148.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.27 0.3
-
 GFA pH 8.0, 37°C Bacillus thermoproteolyticus
3.4.24.27 0.4
-
 GFL pH 8.0, 37°C, product FL Bacillus thermoproteolyticus
3.4.24.27 0.6
-
 GFA pH 8.0, 37°C, 5 M glycerol Bacillus thermoproteolyticus
3.4.24.27 0.7
-
 GFL pH 8.0, 37°C, 5 M glycerol, product GL Bacillus thermoproteolyticus
3.4.24.27 0.7
-
 GFSA pH 8.0, 37°C, product FSA Bacillus thermoproteolyticus
3.4.24.27 1.2
-
 GFSA pH 8.0, 37°C, 5 M glycerol, product GF Bacillus thermoproteolyticus
3.4.24.27 1.3
-
 GFS pH 8.0, 37°C Bacillus thermoproteolyticus
3.4.24.27 1.8
-
 GFL pH 8.0, 37°C, 5 M glycerol, product FL Bacillus thermoproteolyticus
3.4.24.27 2.5
-
 GFL pH 8.0, 37°C, 5 M glycerol, product GF Bacillus thermoproteolyticus
3.4.24.27 3.5
-
 GFS pH 8.0, 37°C, 5 M glycerol Bacillus thermoproteolyticus
3.4.24.27 6.8
-
 GFSA pH 8.0, 37°C, 5 M glycerol, product FSA Bacillus thermoproteolyticus
3.4.24.27 12
-
 GFSA pH 8.0, 37°C, 5 M glycerol, product GF Bacillus thermoproteolyticus

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.27 Bacillus thermoproteolyticus
-
 Rokko
-
3.4.24.27 Bacillus thermoproteolyticus Rokko
-
 Rokko
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.27 GFA + H2O
-
 Bacillus thermoproteolyticus Phe-Ala + Gly
-
 ?
3.4.24.27 GFS + H2O
-
 Bacillus thermoproteolyticus Gly + Phe-Ser
-
 ?
3.4.24.27 GFSA + H2O
-
 Bacillus thermoproteolyticus Gly-Phe + Phe-Ser-Ala + Gly + Ser-Ala
-
 ?
3.4.24.27 Gly-Phe-Leu + H2O
-
 Bacillus thermoproteolyticus Phe-Leu + Gly-Phe + Gly + Leu
-
 ?
3.4.24.27 H-Gly-Phe-Ala-OH + H2O
-
 Bacillus thermoproteolyticus ?
-
 ?
3.4.24.27 H-Gly-Phe-Ala-OH + H2O
-
 Bacillus thermoproteolyticus Rokko ?
-
 ?
3.4.24.27 H-Gly-Phe-Leu-OH + H2O
-
 Bacillus thermoproteolyticus ?
-
 ?
3.4.24.27 H-Gly-Phe-Leu-OH + H2O
-
 Bacillus thermoproteolyticus Rokko ?
-
 ?
3.4.24.27 H-Gly-Phe-Ser-Ala-Lys-Asn-Gln-Ser-Asn-Gln-Arg-OH + H2O
-
 Bacillus thermoproteolyticus ?
-
 ?
3.4.24.27 H-Gly-Phe-Ser-Ala-Lys-Asn-Gln-Ser-Asn-Gln-Arg-OH + H2O
-
 Bacillus thermoproteolyticus Rokko ?
-
 ?
3.4.24.27 H-Gly-Phe-Ser-Ala-Lys-Asn-Gln-Ser-OH + H2O
-
 Bacillus thermoproteolyticus ?
-
 ?
3.4.24.27 H-Gly-Phe-Ser-Ala-Lys-Asn-Gln-Ser-OH + H2O
-
 Bacillus thermoproteolyticus Rokko ?
-
 ?
3.4.24.27 H-Gly-Phe-Ser-OH + H2O
-
 Bacillus thermoproteolyticus ?
-
 ?
3.4.24.27 H-Gly-Phe-Ser-OH + H2O
-
 Bacillus thermoproteolyticus Rokko ?
-
 ?
3.4.24.27 H-Gly-Ser-Ala-OH + H2O
-
 Bacillus thermoproteolyticus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.27 proteinase type X
-
 Bacillus thermoproteolyticus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.24.27 0.006
-
 GFL pH 8.0, 37°C, 5 M glycerol, product GF Bacillus thermoproteolyticus
3.4.24.27 0.013
-
 GFL pH 8.0, 37°C, product GF Bacillus thermoproteolyticus
3.4.24.27 0.026
-
 GFL pH 8.0, 37°C, 5 M glycerol, product FL Bacillus thermoproteolyticus
3.4.24.27 0.035
-
 GFS pH 8.0, 37°C Bacillus thermoproteolyticus
3.4.24.27 0.045
-
 GFL pH 8.0, 37°C, product FL Bacillus thermoproteolyticus
3.4.24.27 0.05
-
 GFS pH 8.0, 37°C, 5 M glycerol Bacillus thermoproteolyticus
3.4.24.27 0.06
-
 GFA pH 8.0, 37°C Bacillus thermoproteolyticus
3.4.24.27 0.07
-
 GFSA pH 8.0, 37°C, product FSA Bacillus thermoproteolyticus
3.4.24.27 0.076
-
 GFA pH 8.0, 37°C, 5 M glycerol Bacillus thermoproteolyticus
3.4.24.27 0.173
-
 GFSA pH 8.0, 37°C, 5 M glycerol, product FSA Bacillus thermoproteolyticus
3.4.24.27 0.3
-
 GFSA pH 8.0, 37°C, product GF Bacillus thermoproteolyticus
3.4.24.27 0.591
-
 GFSA pH 8.0, 37°C, 5 M glycerol, product GF Bacillus thermoproteolyticus
3.4.24.27 6.08
-
 GFSA pH 8.0, 37°C, 5 M glycerol, product GF Bacillus thermoproteolyticus