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Literature summary extracted from

  • Semenova, M.V.; Grishutin, S.G.; Gusakov, A.V.; Okunev, O.N.; Sinitsyn, A.P.
    Isolation and properties of pectinases from the fungus Aspergillus japonicus (2003), Biochemistry (Moscow), 68, 559-569.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.15 additional information
-
 additional information Km value for polygalacturonate is 1.03 g/l with polygalacturonase I. Km value for polygalacturonate is 1.03 g/L with polygalacturonase II Aspergillus japonicus
4.2.2.10 additional information
-
 additional information Km values of pectin substrates with different degree of methylation Aspergillus japonicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.15 NaCl maximal activity at 0.1-0.2 M, polygalacturonase I and II Aspergillus japonicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.11 46000
-
 isoform I, SDS-PAGE Aspergillus japonicus
3.1.1.11 47000
-
 isoform II, SDS-PAGE Aspergillus japonicus
3.2.1.15 38000
-
 x * 38000, polygalacturonase I, SDS-PAGE Aspergillus japonicus
3.2.1.15 65000
-
 x * 65000, polygalacturonase II, SDS-PAGE Aspergillus japonicus
4.2.2.10 50000
-
 x * 50000, SDS-PAGE Aspergillus japonicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.11 pectin + H2O Aspergillus japonicus
-
 methanol + pectate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.11 Aspergillus japonicus
-
-
-
3.2.1.15 Aspergillus japonicus
-
-
-
4.2.2.10 Aspergillus japonicus
-
 mutant strain
-
4.2.2.10 Aspergillus japonicus mutant
-
 mutant strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.11 two isoforms, homogeneity Aspergillus japonicus
3.2.1.15
-
 Aspergillus japonicus
4.2.2.10 263fold Aspergillus japonicus

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.2.10 [6-O-methyl-alpha-D-GalpA]n = [6-O-methyl-alpha-D-GalpA]m + 4-deoxy-6-O-methyl-alpha-D-galact-4-enuronosy-[6-O-methyl-alpha-D-GalpA]n-m-1 acts antagonistic to pectinesterase, EC 3.1.1.11 Aspergillus japonicus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.11 pectin + H2O
-
 Aspergillus japonicus methanol + pectate
-
 ?
3.2.1.15 polygalacturonate + H2O
-
 Aspergillus japonicus ?
-
 ?
4.2.2.10 additional information no activity with polygalacturonic acid, acts antagonistic to pectinesterase, EC 3.1.1.11 Aspergillus japonicus ?
-
 ?
4.2.2.10 additional information no activity with polygalacturonic acid, acts antagonistic to pectinesterase, EC 3.1.1.11 Aspergillus japonicus mutant ?
-
 ?
4.2.2.10 pectin citrus pectin, activity increases with increasing degree of methylation of the substrate Aspergillus japonicus DELTA4,5-unsaturated oligosaccharides
-
 ?
4.2.2.10 pectin citrus pectin, activity increases with increasing degree of methylation of the substrate Aspergillus japonicus mutant DELTA4,5-unsaturated oligosaccharides
-
 ?
4.2.2.10 pectin 26% methylated
-
 Aspergillus japonicus ?
-
 ?
4.2.2.10 pectin 26% methylated
-
 Aspergillus japonicus mutant ?
-
 ?
4.2.2.10 pectin 65% methylated
-
 Aspergillus japonicus ?
-
 ?
4.2.2.10 pectin 65% methylated
-
 Aspergillus japonicus mutant ?
-
 ?
4.2.2.10 pectin 89% methylated
-
 Aspergillus japonicus ?
-
 ?
4.2.2.10 pectin 89% methylated
-
 Aspergillus japonicus mutant ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.15 ? x * 38000, polygalacturonase I, SDS-PAGE Aspergillus japonicus
3.2.1.15 ? x * 65000, polygalacturonase II, SDS-PAGE Aspergillus japonicus
4.2.2.10 ? x * 50000, SDS-PAGE Aspergillus japonicus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.15 endo-polygalacturonase I
-
 Aspergillus japonicus
3.2.1.15 endo-polygalacturonase II
-
 Aspergillus japonicus
3.2.1.15 polygalacturonase I
-
 Aspergillus japonicus
3.2.1.15 polygalacturonase II
-
 Aspergillus japonicus
4.2.2.10 pectin lyase
-
 Aspergillus japonicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.10 40
-
 assay at Aspergillus japonicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.11 40 50 stable Aspergillus japonicus
3.2.1.15 30
-
 about 40% inactivation of polygalacturonase I after 60 min, about 10% inactivation of polygalacturonase II after 60 min Aspergillus japonicus
3.2.1.15 40
-
 about 55% inactivation of polygalacturonase I after 60 min, about 50% inactivation of polygalacturonase II after 60 min Aspergillus japonicus
3.2.1.15 50
-
 complete inactivation of polygalacturonase I after 10 min, complete inactivation of polygalacturonase II after 40 min Aspergillus japonicus
4.2.2.10 40
-
 3 h, completely stable Aspergillus japonicus
4.2.2.10 50
-
 3 h, loss of 10% activity Aspergillus japonicus
4.2.2.10 60
-
 3 h, loss of 55% activity Aspergillus japonicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.15 26
-
 Polygalacturonate polygalacturonase II Aspergillus japonicus
3.2.1.15 90
-
 Polygalacturonate polygalacturonase I Aspergillus japonicus
4.2.2.10 20
-
 pectin 26% methylated pH 5.0, 40°C Aspergillus japonicus
4.2.2.10 77
-
 pectin 65% methylated pH 5.0, 40°C Aspergillus japonicus
4.2.2.10 86
-
 pectin 89% methylated pH 5.0, 40°C Aspergillus japonicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.11 4.5
-
-
 Aspergillus japonicus
3.2.1.15 4
-
 polygalacturonase II Aspergillus japonicus
3.2.1.15 5
-
 polygalacturonase I Aspergillus japonicus
4.2.2.10 5
-
 assay at Aspergillus japonicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.11 3.5 5.5 about 50% activity at pH 3.5 and pH 5.5 Aspergillus japonicus
3.2.1.15 3.5 5.5 pH 3.5: about 50% of maximal activity, pH 5.5: about 60% of maximal activity, polygalacturonase II Aspergillus japonicus
3.2.1.15 4.5 5.5 pH 4.5: about 50% of maximal activity, pH 5.5: about 40% of maximal activity, polygalacturonase I Aspergillus japonicus
4.2.2.10 4 6.5 50% of maximal activity at pH 4.0 and pH 6.5 Aspergillus japonicus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.11 Aspergillus japonicus isoelectric focusing
-
 3.8
4.2.2.10 Aspergillus japonicus isoelectric focusing
-
 4.2