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Literature summary extracted from
- Isolation and characterization of glutamyl endopeptidase 2 from Bacillus intermedius 3-19 (2003), Biochemistry (Moscow), 68, 1217-1224.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.19 | 4-chloromercuribenzoate | slight inhibition | Bacillus intermedius |  |
| 3.4.21.19 | diisopropylfluorophosphate | complete, irreversible inhibition | Bacillus intermedius | |
| 3.4.21.19 | additional information | no inhibition by EDTA, benzamidine, duck ovomucoid, inhibitor from marine anemone, soybean trypsin inhibitor | Bacillus intermedius | |
| 3.4.21.19 | PMSF | slight inhibition | Bacillus intermedius | |
| 3.4.21.82 | diisopropylfluorophosphate | - |
Bacillus intermedius | |
| 3.4.21.82 | additional information | little or no inhibition with PMSF, EDTA, benzamidine, p-CMB, duck ovomucoid, inhibitor from marine anemone, and soybean trypsin inhibitor | Bacillus intermedius |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.19 | 0.5 | - |
benzyloxycarbonyl-Glu-4-nitroanilide | pH 8.5 | Bacillus intermedius | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.21.19 | extracellular | secretion to the culture medium during the late stages of growth | Bacillus intermedius | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.19 | Ca2+ | stabilizes | Bacillus intermedius | |
| 3.4.21.82 | Ca2+ | stabilizes | Bacillus intermedius | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.19 | 26500 | - |
x * 26500, SDS-PAGE | Bacillus intermedius |
| 3.4.21.82 | 26500 | - |
gel filtration | Bacillus intermedius |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.19 | Bacillus intermedius | - |
strain 3-19, glutamyl endopaptidase 2 | - |
| 3.4.21.19 | Bacillus intermedius Mrz 19 | - |
strain 3-19, glutamyl endopaptidase 2 | - |
| 3.4.21.82 | Bacillus intermedius | - |
strain 3-19 | - |
| 3.4.21.82 | Bacillus intermedius Mrz 19 | - |
strain 3-19 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.21.19 | 1275fold, to homogeneity | Bacillus intermedius |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.19 | 1.02 | - |
purified enzyme | Bacillus intermedius |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.19 | benzyloxycarbonyl-Ala-Ala-Leu-Asp-4-nitroanilide + H2O | - |
Bacillus intermedius | benzyloxycarbonyl-Ala-Ala-Leu-Asp + 4-nitroaniline | - |
? | |
| 3.4.21.19 | benzyloxycarbonyl-Ala-Ala-Leu-Glu-4-nitroanilide + H2O | - |
Bacillus intermedius | benzyloxycarbonyl-Ala-Ala-Leu-Glu + 4-nitroaniline | - |
? | |
| 3.4.21.19 | benzyloxycarbonyl-Ala-Ala-Met-Asp-4-nitroanilide + H2O | - |
Bacillus intermedius | benzyloxycarbonyl-Ala-Ala-Met-Asp + 4-nitroaniline | - |
? | |
| 3.4.21.19 | benzyloxycarbonyl-Ala-Ala-Met-Glu-4-nitroanilide + H2O | - |
Bacillus intermedius | benzyloxycarbonyl-Ala-Ala-Met-Glu + 4-nitroaniline | - |
? | |
| 3.4.21.19 | benzyloxycarbonyl-Ala-Ala-Phe-Asp-4-nitroanilide + H2O | - |
Bacillus intermedius | benzyloxycarbonyl-Ala-Ala-Phe-Asp + 4-nitroaniline | - |
? | |
| 3.4.21.19 | benzyloxycarbonyl-Ala-Ala-Phe-Glu-4-nitroanilide + H2O | preferred peptide substrate | Bacillus intermedius | benzyloxycarbonyl-Ala-Ala-Phe-Glu + 4-nitroaniline | - |
? | |
| 3.4.21.19 | benzyloxycarbonyl-Ala-Ala-Trp-Asp-4-nitroanilide + H2O | - |
Bacillus intermedius | benzyloxycarbonyl-Ala-Ala-Trp-Asp + 4-nitroaniline | - |
? | |
| 3.4.21.19 | benzyloxycarbonyl-Ala-Ala-Trp-Glu-4-nitroanilide + H2O | - |
Bacillus intermedius | benzyloxycarbonyl-Ala-Ala-Trp-Glu + 4-nitroaniline | - |
? | |
| 3.4.21.19 | benzyloxycarbonyl-Glu-4-nitroanilide + H2O | - |
Bacillus intermedius | benzyloxycarbonyl-Glu + 4-nitroaniline | - |
? | |
| 3.4.21.19 | benzyloxycarbonyl-Gly-Ala-Ala-Asp-4-nitroanilide + H2O | - |
Bacillus intermedius | benzyloxycarbonyl-Gly-Ala-Ala-Asp + 4-nitroaniline | - |
? | |
| 3.4.21.19 | benzyloxycarbonyl-Gly-Ala-Ala-Glu-4-nitroanilide + H2O | - |
Bacillus intermedius | benzyloxycarbonyl-Gly-Ala-Ala-Glu + 4-nitroaniline | - |
? | |
| 3.4.21.19 | casein + H2O | - |
Bacillus intermedius | ? | - |
? | |
| 3.4.21.19 | insulin A-chain + H2O | hydrolysis of Glu4-Gln5, Glu17-Asp18, and Cys11-Ser12 | Bacillus intermedius | peptide fragments | - |
? | |
| 3.4.21.19 | insulin B-chain + H2O | hydrolysis of Glu13-Ala14, Glu21-Arg22, Cys7-Gly8, and Cys19-Gly20 | Bacillus intermedius | peptide fragments | - |
? | |
| 3.4.21.19 | additional information | substrate specificity, preferrence for Glu-cleavage site before Asp-cleavage site | Bacillus intermedius | ? | - |
? | |
| 3.4.21.82 | benzlyoxycarbonyl-Ala-Ala-Leu-Glu-p-nitroanilide + H2O | - |
Bacillus intermedius | ? | - |
? | |
| 3.4.21.82 | benzlyoxycarbonyl-Ala-Ala-Met-Glu-p-nitroanilide + H2O | - |
Bacillus intermedius | ? | - |
? | |
| 3.4.21.82 | benzlyoxycarbonyl-Ala-Ala-Met-Glu-p-nitroanilide + H2O | - |
Bacillus intermedius Mrz 19 | ? | - |
? | |
| 3.4.21.82 | benzlyoxycarbonyl-Ala-Ala-Phe-Glu-p-nitroanilide + H2O | - |
Bacillus intermedius | ? | - |
? | |
| 3.4.21.82 | benzlyoxycarbonyl-Ala-Ala-Trp-Glu-p-nitroanilide + H2O | - |
Bacillus intermedius | ? | - |
? | |
| 3.4.21.82 | benzlyoxycarbonyl-Ala-Ala-Trp-Glu-p-nitroanilide + H2O | - |
Bacillus intermedius Mrz 19 | ? | - |
? | |
| 3.4.21.82 | benzlyoxycarbonyl-Glu-p-nitroanilide + H2O | - |
Bacillus intermedius | ? | - |
? | |
| 3.4.21.82 | benzlyoxycarbonyl-Gly-Ala-Ala-Glu-p-nitroanilide + H2O | - |
Bacillus intermedius | ? | - |
? | |
| 3.4.21.82 | casein + H2O | - |
Bacillus intermedius | hydrolyzed casein | - |
? | |
| 3.4.21.82 | casein + H2O | - |
Bacillus intermedius Mrz 19 | hydrolyzed casein | - |
? | |
| 3.4.21.82 | insulin + H2O | hydrolyzes Glu4-Gln5, Glu17-Asp18, and Cys11-Ser12 bonds in the oxidized A-chain of insulin and Glu13-Ala14, Glu21-Arg22, Cys7-Gly8, and Cys19-Gly20 bonds in the oxidized B-chain of insulin | Bacillus intermedius | ? | - |
? | |
| 3.4.21.82 | insulin + H2O | hydrolyzes Glu4-Gln5, Glu17-Asp18, and Cys11-Ser12 bonds in the oxidized A-chain of insulin and Glu13-Ala14, Glu21-Arg22, Cys7-Gly8, and Cys19-Gly20 bonds in the oxidized B-chain of insulin | Bacillus intermedius Mrz 19 | ? | - |
? | |
| 3.4.21.82 | additional information | does not hydrolyze the subtilisin substrate benzyloxycarbonyl-Ala-Ala-Leu-p-nitroanilide, only little activity with benzyloxycarbonyl-Ala-Ala-Trp-Asp-p-nitroanilide, benzyloxycarbonyl-Ala-Ala-Leu-Asp-p-nitroanilide, benzyloxycarbonyl-Ala-Ala-Phe-Asp-p-nitroanilide, benzyloxycarbonyl-Ala-Ala-Met-Asp-p-nitroanilide, and benzyloxycarbonyl-Gly-Ala-Ala-Asp-p-nitroanilide | Bacillus intermedius | ? | - |
? | |
| 3.4.21.82 | additional information | does not hydrolyze the subtilisin substrate benzyloxycarbonyl-Ala-Ala-Leu-p-nitroanilide, only little activity with benzyloxycarbonyl-Ala-Ala-Trp-Asp-p-nitroanilide, benzyloxycarbonyl-Ala-Ala-Leu-Asp-p-nitroanilide, benzyloxycarbonyl-Ala-Ala-Phe-Asp-p-nitroanilide, benzyloxycarbonyl-Ala-Ala-Met-Asp-p-nitroanilide, and benzyloxycarbonyl-Gly-Ala-Ala-Asp-p-nitroanilide | Bacillus intermedius Mrz 19 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.21.19 | ? | x * 26500, SDS-PAGE | Bacillus intermedius |
| 3.4.21.82 | monomer | 1 * 26500, SDS-PAGE | Bacillus intermedius |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.21.19 | glutamyl endopeptidase 2 | - |
Bacillus intermedius |
| 3.4.21.82 | glutamyl endopeptidase 2 | - |
Bacillus intermedius |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.19 | 50 | - |
in absence of Ca2+ | Bacillus intermedius |
| 3.4.21.19 | 55 | - |
in presence of Ca2+ | Bacillus intermedius |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.19 | 22 | 37 | stable, in absence of Ca2+ | Bacillus intermedius |
| 3.4.21.19 | 37 | 55 | stable, in presence of Ca2+ | Bacillus intermedius |
| 3.4.21.19 | 50 | - |
in absence of Ca2+, loss of 55% activity | Bacillus intermedius |
| 3.4.21.19 | 55 | - |
in absence of Ca2+, loss of 90% activity | Bacillus intermedius |
| 3.4.21.82 | 22 | 37 | without Ca2+, stable at 37°C, but activity decreases at 50°C and 55°C by 55% and 90% respectively | Bacillus intermedius |
| 3.4.21.82 | 37 | 55 | with Ca2+, highly thermostable, heating at 37°C, 50°C, and 55°C has no effect on activity | Bacillus intermedius |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.19 | 81 | - |
benzyloxycarbonyl-Glu-4-nitroanilide | pH 8.5 | Bacillus intermedius | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.19 | 7.2 | - |
enzyme possesses 2 optima at pH 7.2 and pH 9.5 for reaction with casein | Bacillus intermedius |
| 3.4.21.19 | 8.5 | - |
reaction with benzyloxycarbonyl-Glu-4-nitroanilide | Bacillus intermedius |
| 3.4.21.19 | 9.5 | - |
enzyme possesses 2 optima at pH 7.2 and pH 9.5 for reaction with casein | Bacillus intermedius |
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