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Literature summary extracted from

  • Lamothe, M.; Chang, F.J.; Balashova, N.; Shirokov, R.; Beuve, A.
    Functional Characterization of Nitric Oxide and YC-1 Activation of Soluble Guanylyl Cyclase: Structural Implication for the YC-1 Binding Site? (2004), Biochemistry, 43, 3039-3048.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.6.1.2 1-benzyl-3-(hydroxymethyl-2-furyl)indazole trivial name YC-1, allosteric activator, synergistically increases the catalytic activity in the presence of NO Rattus norvegicus
4.6.1.2 N-(beta-D-glucopyranosyl-N2-acetyl-S-nitroso-D,L-penicillaminamide) NO donor Rattus norvegicus
4.6.1.2 NO binds at beta1 subunit heme, several hundred fold increase in activity Rattus norvegicus
4.6.1.2 sodium nitroprusside NO donor Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.6.1.2 expression of sGC in Sf9 insect cells and COS-7 cells Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
4.6.1.2 C238S no change in cGMP production Rattus norvegicus
4.6.1.2 C243S no change in cGMP production Rattus norvegicus
4.6.1.2 C594D no 1-benzyl-3-(hydroxymethyl-2-furyl)indazole activation, no synergy with NO Rattus norvegicus
4.6.1.2 C594D/E525K loss of activity Rattus norvegicus
4.6.1.2 C594Y reduced level of NO and 1-benzyl-3-(hydroxymethyl-2-furyl)indazole activation Rattus norvegicus
4.6.1.2 D477A reduced level of NO and 1-benzyl-3-(hydroxymethyl-2-furyl)indazole activation Rattus norvegicus
4.6.1.2 M537N high level of 1-benzyl-3-(hydroxymethyl-2-furyl)indazole activation Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.6.1.2 0.025
-
 GTP 30°C, pH 8.0, in the presence of N-(beta-D-glucopyranosyl-N2-acetyl-S-nitroso-D,L-penicillaminamide) and YC-1 Rattus norvegicus
4.6.1.2 0.039
-
 GTP 30°C, pH 8.0, in the presence of YC-1 Rattus norvegicus
4.6.1.2 0.061
-
 GTP 30°C, pH 8.0, in the presence of N-(beta-D-glucopyranosyl-N2-acetyl-S-nitroso-D,L-peniciiaminamide) Rattus norvegicus
4.6.1.2 0.12
-
 GTP 30°C, pH 8.0, basal activity Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.6.1.2 GTP Rattus norvegicus
-
 3',5'-cyclic GMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.2 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.2 recombinant sGC Rattus norvegicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.6.1.2 0.12
-
 basal activity Rattus norvegicus
4.6.1.2 9.1
-
 in the presence of 0.1 mM N-(beta-D-glucopyranosyl-N2-acetyl-S-nitroso-D,L-penicillaminamide) Rattus norvegicus
4.6.1.2 12.1
-
 in the presence of 0.1 mM N-(beta-D-glucopyranosyl-N2-acetyl-S-nitroso-D,L-penicillaminamide) and YC-1 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.2 GTP
-
 Rattus norvegicus 3',5'-cyclic GMP + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.6.1.2 sGC
-
 Rattus norvegicus
4.6.1.2 soluble guanylyl cyclase
-
 Rattus norvegicus