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Literature summary extracted from

  • Pereira, M.P.; Brown, E.D.
    Bifunctional catalysis by CDP-ribitol synthase: convergent recruitment of the reductase and cytidylyltransferase activities in Haemophilus influenzae and Staphylococcus aureus (2004), Biochemistry, 43, 11802-11812.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.405 overexpression of TarI and TarJ individually from their respective genes using the T7 expression system Staphylococcus aureus
2.7.7.40 overexpression of TarI and TarJ individually from their respective genes using the T7 expression system Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.405 2-[5-Amino-2-(4-fluoro-phenyl)-6-oxo-6H-pyrimidin-1-yl]-N-(1-benzyl-2-oxo-2-thiazol-2-yl-ethyl)-acetamide
-
 Haemophilus influenzae
1.1.1.405 2-[5-Amino-2-(4-fluoro-phenyl)-6-oxo-6H-pyrimidin-1-yl]-N-(1-benzyl-2-oxo-2-thiazol-2-yl-ethyl)-acetamide
-
 Staphylococcus aureus
1.1.1.405 D-ribitol 5-phosphate
-
 Haemophilus influenzae
1.1.1.405 D-ribitol 5-phosphate
-
 Staphylococcus aureus
1.1.1.405 NADP+ competitive versus NADPH Haemophilus influenzae
1.1.1.405 NADP+ competitive versus NADPH Staphylococcus aureus
2.7.7.40 CDP-ribitol competitive versus CTP Haemophilus influenzae
2.7.7.40 CDP-ribitol competitive versus CTP Staphylococcus aureus
2.7.7.40 diphosphate
-
 Haemophilus influenzae
2.7.7.40 diphosphate
-
 Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.405 0.00674
-
 NADPH
-
 Staphylococcus aureus
1.1.1.405 0.00706
-
 NADPH
-
 Haemophilus influenzae
1.1.1.405 0.0285
-
 ribulose 5-phosphate
-
 Staphylococcus aureus
1.1.1.405 0.106
-
 ribulose 5-phosphate
-
 Haemophilus influenzae
2.7.7.40 0.00851
-
 CTP pH 7.2 Haemophilus influenzae
2.7.7.40 0.0147
-
 D-ribitol 5-phosphate pH 7.2 Haemophilus influenzae
2.7.7.40 0.0789
-
 CTP pH 7.2 Staphylococcus aureus
2.7.7.40 1.28
-
 D-ribitol 5-phosphate pH 7.2 Staphylococcus aureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.405 26000
-
 2 * 26000 + 2 * 41000, tarI encodes an orthologue of the cytidylyltransferase domain of Bcs1 from Haemophilus influenzae and tarJ encodes an analogue of the reductase domain of Bcs1, TarI and TyrJ form a functional CDP-ribitol synthase complex, SDS-PAGE Staphylococcus aureus
1.1.1.405 41000
-
 2 * 26000 + 2 * 41000, tarI encodes an orthologue of the cytidylyltransferase domain of Bcs1 from Haemophilus influenzae and tarJ encodes an analogue of the reductase domain of Bcs1, TarI and TyrJ form a functional CDP-ribitol synthase complex, SDS-PAGE Staphylococcus aureus
1.1.1.405 53190
-
 2 * 53190, calculation from amino acid composition Haemophilus influenzae
1.1.1.405 106400
-
 amino acid composition Haemophilus influenzae
1.1.1.405 129000
-
 calculation from gel filtration and sedimentation velocity data Staphylococcus aureus
2.7.7.40 26000
-
 2 * 26000 + 2 * 41000, TarI2J2, tarI encodes an orthologue of the cytidylyltransferase domain of Bcs1 from Haemophilus influenzae and tarJ encodes an analogue of the reductase domain of Bcs1, TarI and TyrJ form a functional CDP-ribitol synthase complex, SDS-PAGE Staphylococcus aureus
2.7.7.40 41000
-
 2 * 26000 + 2 * 41000, TarI2J2, tarI encodes an orthologue of the cytidylyltransferase domain of Bcs1 from Haemophilus influenzae and tarJ encodes an analogue of the reductase domain of Bcs1, TarI and TyrJ form a functional CDP-ribitol synthase complex, SDS-PAGE Staphylococcus aureus
2.7.7.40 53190
-
 2 * 53190, calculation from amino acid composition Haemophilus influenzae
2.7.7.40 106400
-
 amino acid composition Haemophilus influenzae
2.7.7.40 129000
-
 calculation from gel filtration and sedimentation velocity data Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.405 Haemophilus influenzae
-
-
-
1.1.1.405 Staphylococcus aureus
-
-
-
2.7.7.40 Haemophilus influenzae
-
-
-
2.7.7.40 Staphylococcus aureus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.405
-
 Staphylococcus aureus
2.7.7.40
-
 Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.405 D-ribulose 5-phosphate + NADPH + H+ tarI encodes an orthologue of the cytidylyltransferase domain of Bcs1 from Haemophilus influenzae and tarJ encodes an analogue of the reductase domain of Bcs1, TarI and TyrJ form a functional CDP-ribitol synthase complex. Release and free diffusion of the intermediate ribitol 5-phosphate without channelling between active sites, ordered bi-bi mechanism Staphylococcus aureus D-ribitol 5-phosphate + NADP+
-
 ?
1.1.1.405 D-ribulose 5-phosphate + NADPH + H+ the data suggest that NADPH binds first and ribulose 5-phosphate binds second. Bifunction enzyme catalyzing the reactions of EC 1.1.1.137 and EC 2.7.7.40, ordered bi-bi mechanism Haemophilus influenzae D-ribitol 5-phosphate + NADP+
-
 ?
2.7.7.40 CTP + D-ribitol 5-phosphate ordered bi-bi mechanism Haemophilus influenzae diphosphate + CDP-ribitol
-
 ?
2.7.7.40 CTP + D-ribitol 5-phosphate tarI encodes an orthologue of the cytidylyltransferase domain of Bcs1 from Haemophilus influenzae and tarJ encodes an analogue of the reductase domain of Bcs1, TarI and TyrJ form a functional CDP-ribitol synthase complex. Release and free diffusion of the intermediate ribitol 5-phosphate without channelling between active sites, ordered bi-bi mechanism Staphylococcus aureus diphosphate + CDP-ribitol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.405 ? 2 * 26000 + 2 * 41000, tarI encodes an orthologue of the cytidylyltransferase domain of Bcs1 from Haemophilus influenzae and tarJ encodes an analogue of the reductase domain of Bcs1, TarI and TyrJ form a functional CDP-ribitol synthase complex, SDS-PAGE Staphylococcus aureus
1.1.1.405 dimer 2 * 53190, calculation from amino acid composition Haemophilus influenzae
2.7.7.40 ? 2 * 26000 + 2 * 41000, TarI2J2, tarI encodes an orthologue of the cytidylyltransferase domain of Bcs1 from Haemophilus influenzae and tarJ encodes an analogue of the reductase domain of Bcs1, TarI and TyrJ form a functional CDP-ribitol synthase complex, SDS-PAGE Staphylococcus aureus
2.7.7.40 ? 2 * 53190, calculation from amino acid composition Haemophilus influenzae

Synonyms

EC Number Synonyms Comment Organism
1.1.1.405 Bcs1
-
 Haemophilus influenzae
1.1.1.405 TarIJ
-
 Staphylococcus aureus
2.7.7.40 Bcs1
-
 Haemophilus influenzae
2.7.7.40 cytidylyltransferase
-
 Staphylococcus aureus
2.7.7.40 cytidylyltransferase
-
 Haemophilus influenzae
2.7.7.40 TarIJ
-
 Staphylococcus aureus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.405 0.03 0.55 NADPH
-
 Haemophilus influenzae
1.1.1.405 0.79
-
 NADPH
-
 Staphylococcus aureus
1.1.1.405 6.08
-
 NADPH
-
 Staphylococcus aureus
1.1.1.405 7.41
-
 NADPH
-
 Haemophilus influenzae
2.7.7.40 2.8
-
 CTP pH 7.2 Staphylococcus aureus
2.7.7.40 23.5
-
 CTP pH 7.2 Haemophilus influenzae
2.7.7.40 76.8
-
 CTP pH 7.2 Staphylococcus aureus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.405 additional information
-
 2-[5-Amino-2-(4-fluoro-phenyl)-6-oxo-6H-pyrimidin-1-yl]-N-(1-benzyl-2-oxo-2-thiazol-2-yl-ethyl)-acetamide Ki(intercept) and Ki(slope)-values Staphylococcus aureus
1.1.1.405 additional information
-
 2-[5-Amino-2-(4-fluoro-phenyl)-6-oxo-6H-pyrimidin-1-yl]-N-(1-benzyl-2-oxo-2-thiazol-2-yl-ethyl)-acetamide Ki(intercept) and Ki(slope)-values Haemophilus influenzae
2.7.7.40 additional information
-
 additional information Ki(intercept) and Ki(slope)-values Staphylococcus aureus
2.7.7.40 additional information
-
 additional information Ki(intercept) and Ki(slope)-values Haemophilus influenzae