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Literature summary extracted from

  • Naught, L.E.; Regni, C.; Beamer, L.J.; Tipton, P.A.
    Roles of active site residues in Pseudomonas aeruginosa phosphomannomutase/phosphoglucomutase (2003), Biochemistry, 42, 9946-9951.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.2.2 expression in Escherichia coli Pseudomonas aeruginosa
5.4.2.8 expression in Escherichia coli Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
5.4.2.2 H109Q 6% of wild-type activity Pseudomonas aeruginosa
5.4.2.2 H308N 100% of wild-type activity Pseudomonas aeruginosa
5.4.2.2 H308N/H329N 5% of wild-type activity Pseudomonas aeruginosa
5.4.2.2 H329N 6% of wild-type activity Pseudomonas aeruginosa
5.4.2.2 K118L 4% of wild-type activity Pseudomonas aeruginosa
5.4.2.2 K118L/H109Q 5% of wild-type activity Pseudomonas aeruginosa
5.4.2.2 R20A 12% of wild-type activity Pseudomonas aeruginosa
5.4.2.2 R247A 9% of wild-type activity Pseudomonas aeruginosa
5.4.2.2 S108A 12% of wild-type activity Pseudomonas aeruginosa
5.4.2.2 S108A/H109Q 6% of wild-type activity Pseudomonas aeruginosa
5.4.2.2 S108A/H308N 3% of wild-type activity Pseudomonas aeruginosa
5.4.2.2 S108A/H329N no activity Pseudomonas aeruginosa
5.4.2.2 S108D 7% of wild-type activity Pseudomonas aeruginosa
5.4.2.2 S108V 1% of wild-type activity Pseudomonas aeruginosa
5.4.2.8 H109Q 6% of wild-type activity Pseudomonas aeruginosa
5.4.2.8 H308N 100% of wild-type activity Pseudomonas aeruginosa
5.4.2.8 H308N/H329N 5% of wild-type activity Pseudomonas aeruginosa
5.4.2.8 H329N 6% of wild-type activity Pseudomonas aeruginosa
5.4.2.8 K118L 4% of wild-type activity Pseudomonas aeruginosa
5.4.2.8 K118L/H109Q 5% of wild-type activity Pseudomonas aeruginosa
5.4.2.8 R20A 12% of wild-type activity Pseudomonas aeruginosa
5.4.2.8 R247A 9% of wild-type activity Pseudomonas aeruginosa
5.4.2.8 S108A 12% of wild-type activity Pseudomonas aeruginosa
5.4.2.8 S108A/H109Q 6% of wild-type activity Pseudomonas aeruginosa
5.4.2.8 S108A/H308N 3% of wild-type activity Pseudomonas aeruginosa
5.4.2.8 S108A/H329N no activity Pseudomonas aeruginosa
5.4.2.8 S108D 7% of wild-type activity Pseudomonas aeruginosa
5.4.2.8 S108V 1% of wild-type activity Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.2.2 0.0013
-
 alpha-D-glucose 1-phosphate H308N/H329N double mutant Pseudomonas aeruginosa
5.4.2.2 0.0013
-
 alpha-D-glucose 1-phosphate S108A mutant Pseudomonas aeruginosa
5.4.2.2 0.0024
-
 alpha-D-glucose 1-phosphate S108A/H308N double mutant Pseudomonas aeruginosa
5.4.2.2 0.0027
-
 alpha-D-glucose 1-phosphate K118L/H109Q double mutant Pseudomonas aeruginosa
5.4.2.2 0.0028
-
 alpha-D-glucose 1-phosphate R20A mutant Pseudomonas aeruginosa
5.4.2.2 0.0034
-
 alpha-D-glucose 1-phosphate S108D mutant Pseudomonas aeruginosa
5.4.2.2 0.008
-
 alpha-D-glucose 1-phosphate H109Q mutant Pseudomonas aeruginosa
5.4.2.2 0.008
-
 alpha-D-glucose 1-phosphate K118L mutant Pseudomonas aeruginosa
5.4.2.2 0.009
-
 alpha-D-glucose 1-phosphate R247A mutant Pseudomonas aeruginosa
5.4.2.2 0.01
-
 alpha-D-glucose 1-phosphate H308N mutant Pseudomonas aeruginosa
5.4.2.2 0.01
-
 alpha-D-glucose 1-phosphate S108V mutant Pseudomonas aeruginosa
5.4.2.2 0.012
-
 alpha-D-glucose 1-phosphate
-
 Pseudomonas aeruginosa
5.4.2.2 0.016
-
 alpha-D-glucose 1-phosphate H329N mutant Pseudomonas aeruginosa
5.4.2.8 0.0013
-
 glucose 1-phosphate H308N/H329N double mutant Pseudomonas aeruginosa
5.4.2.8 0.0013
-
 glucose 1-phosphate S108A mutant Pseudomonas aeruginosa
5.4.2.8 0.0024
-
 glucose 1-phosphate S108A/H308N double mutant Pseudomonas aeruginosa
5.4.2.8 0.0027
-
 glucose 1-phosphate K118L/H109Q double mutant Pseudomonas aeruginosa
5.4.2.8 0.0028
-
 glucose 1-phosphate R20A mutant Pseudomonas aeruginosa
5.4.2.8 0.0034
-
 glucose 1-phosphate S108D mutant Pseudomonas aeruginosa
5.4.2.8 0.008
-
 glucose 1-phosphate H109Q mutant Pseudomonas aeruginosa
5.4.2.8 0.008
-
 glucose 1-phosphate K118L mutant Pseudomonas aeruginosa
5.4.2.8 0.009
-
 glucose 1-phosphate R247A mutant Pseudomonas aeruginosa
5.4.2.8 0.01
-
 glucose 1-phosphate H308N mutant Pseudomonas aeruginosa
5.4.2.8 0.01
-
 glucose 1-phosphate S108V mutant Pseudomonas aeruginosa
5.4.2.8 0.012
-
 glucose 1-phosphate
-
 Pseudomonas aeruginosa
5.4.2.8 0.016
-
 glucose 1-phosphate H329N mutant Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.2.2 alpha-D-glucose 1-phosphate Pseudomonas aeruginosa
-
 alpha-D-glucose 6-phosphate
-
 r
5.4.2.8 alpha-D-glucose 1-phosphate Pseudomonas aeruginosa
-
 alpha-D-glucose 6-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.4.2.2 Pseudomonas aeruginosa P26276
-
-
5.4.2.8 Pseudomonas aeruginosa P26276
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.2.2 recombinant PGM Pseudomonas aeruginosa
5.4.2.8 recombinant PGM Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.2.2 alpha-D-glucose 1-phosphate
-
 Pseudomonas aeruginosa alpha-D-glucose 6-phosphate
-
 r
5.4.2.2 alpha-D-mannose 1-phosphate
-
 Pseudomonas aeruginosa alpha-D-mannose 6-phosphate
-
 r
5.4.2.8 alpha-D-glucose 1-phosphate
-
 Pseudomonas aeruginosa alpha-D-glucose 6-phosphate
-
 r
5.4.2.8 alpha-D-mannose 1-phosphate
-
 Pseudomonas aeruginosa alpha-D-mannose 6-phosphate
-
 r

Synonyms

EC Number Synonyms Comment Organism
5.4.2.2 phosphomannomutase/phosphoglucomutase
-
 Pseudomonas aeruginosa
5.4.2.2 PMM/PGM
-
 Pseudomonas aeruginosa
5.4.2.8 phosphomannomutase/phosphoglucomutase
-
 Pseudomonas aeruginosa
5.4.2.8 PMM/PGM
-
 Pseudomonas aeruginosa