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Literature summary extracted from

  • Mitsudo, K.; Jayakumar, A.; Henderson, Y.; Frederick, M.J.; Kang, Y.; Wang, M.; El-Naggar, A.K.; Clayman, G.L.
    Inhibition of serine proteinases plasmin, trypsin, subtilisin A, cathepsin G, and elastase by LEKTI: a kinetic analysis (2003), Biochemistry, 42, 3874-3881.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.4 LEKTI the LEKI gene encodes a 15-domain serine proteinase inhibitor, disulfide bonds are important for LEKTI function, noncompetitive mechanism, expression and purification of human LEKTI gene in Sf9 Homo sapiens
3.4.21.7 LEKTI potent noncompetitive inhibitor, recombinant LEKTI is purified using a baculovirus/insect cell expression system Homo sapiens
3.4.21.62 human LEKTI noncompetitive inhibition Bacillus licheniformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.4 0.485
-
Glu-Gly-Arg-4-nitroanilide pH 7.4, 25°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.4 Homo sapiens
-
-
-
3.4.21.7 Homo sapiens
-
-
-
3.4.21.62 Bacillus licheniformis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.4 Glu-Gly-Arg-4-nitroanilide + H2O
-
Homo sapiens ?
-
?
3.4.21.62 succinyl-AAPF-p-nitroanilide + H2O
-
Bacillus licheniformis succinyl-AAPF + p-nitroaniline
-
?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.21.4 0.000849
-
LEKTI pH 7.4, 25°C Homo sapiens
3.4.21.7 0.000027
-
LEKTI
-
Homo sapiens
3.4.21.62 0.000049
-
human LEKTI pH 7.4, 25°C Bacillus licheniformis