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Literature summary extracted from

  • Klenchin, V.A.; Taylor Ringia, E.A.; Gerlt, J.A.; Rayment, I.
    Evolution of enzymatic activity in the enolase superfamily: structural and mutagenic studies of the mechanism of the reaction catalyzed by o-succinylbenzoate synthase from Escherichia coli (2003), Biochemistry, 42, 14427-14433.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.113 expression in Escherichia coli of several recombinant mutant enzymes Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.113 co-crystallization of the K133R mutant with 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylic acid Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.113 K133A inactive mutant Escherichia coli
4.2.1.113 K133R inactive mutant Escherichia coli
4.2.1.113 K133S inactive mutant Escherichia coli
4.2.1.113 K235A inactive mutant Escherichia coli
4.2.1.113 K235R inactive mutant Escherichia coli
4.2.1.113 K235S inactive mutant Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.113 Mg2+ required for activity Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.113 37740
-
 mass spectrometry Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.113 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylic acid Escherichia coli
-
 4-(2-carboxyphenyl)-4-oxobutyrate + H2O
-
 ir
4.2.1.113 additional information Escherichia coli involved in the menaquinone biosynthetic pathway ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.113 Escherichia coli P29208
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.113 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylic acid
-
 Escherichia coli 4-(2-carboxyphenyl)-4-oxobutyrate + H2O
-
 ?
4.2.1.113 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylic acid
-
 Escherichia coli 4-(2-carboxyphenyl)-4-oxobutyrate + H2O
-
 ir
4.2.1.113 additional information involved in the menaquinone biosynthetic pathway Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.113 OSBS
-
 Escherichia coli