Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Vasiljeva, O.; Dolinar, M.; Turk, V.; Turk, B.
    Recombinant human cathepsin H lacking the mini chain is an endopeptidase (2003), Biochemistry, 42, 13522-13528.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.4.22.16 additional information mutant lacking the mini chain, loss of aminopeptidase activity, increase in endopeptidase activity Homo sapiens
3.4.22.16 additional information mutant lacking the mini chain, loss of aminopeptidase activity, increase in endopeptidase activity Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.16 bovine stefin B
-
Homo sapiens
3.4.22.16 chicken cystatin
-
Homo sapiens
3.4.22.16 human stefin A
-
Homo sapiens
3.4.22.16 human stefin B
-
Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.16 0.025
-
benzoyl-L-Phe-L-Val-L-Arg-4-methylcoumaryl-7-amide pH 6.5, 25°C, native enzyme Sus scrofa
3.4.22.16 0.038
-
benzoyl-L-Phe-L-Val-L-Arg-4-methylcoumaryl-7-amide pH 6.5, 25°C, mutant lacking the mini chain Sus scrofa
3.4.22.16 0.082
-
benzoyl-L-Phe-L-Arg-7-amido-4-methylcoumarin pH 6.5, 25°C, mutant lacking the mini chain Sus scrofa
3.4.22.16 0.1
-
L-Arg-4-methylcoumaryl-7-amide pH 6.5, 25°C, native enzyme Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.16 Homo sapiens
-
recombinant protein lacking the mini chain
-
3.4.22.16 Sus scrofa
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.22.16 proteolytic modification mutant lacking the mini chain, autocatalytic processing at pH 5.0, 37°C, processing is accelerated in presence of 0.025 mg/ml of dextran, prolonged activation results in both cases in decrease of enzyme acivtiy Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.16 benzoyl-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O
-
Sus scrofa benzoyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin
-
?
3.4.22.16 benzoyl-L-Phe-L-Val-L-Arg-4-methylcoumaryl-7-amide + H2O
-
Sus scrofa benzoyl-L-Phe-L-Val-L-Arg + 7-amino-4-methylcoumarin
-
?
3.4.22.16 L-Arg-4-methylcoumaryl-7-amide + H2O
-
Sus scrofa L-Arg + 7-amino-4-methylcoumarin
-
?
3.4.22.16 Z-L-Phe-L-Arg-4-methylcoumaryl-7-amide + H2O
-
Sus scrofa benzoyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin
-
?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.22.16 0.000000049
-
chicken cystatin pH 6.5, 25°C, mutant lacking the mini chain Homo sapiens
3.4.22.16 0.000000074
-
human stefin B pH 6.5, 25°C, mutant lacking the mini chain Homo sapiens
3.4.22.16 0.000000099
-
human stefin A pH 6.5, 25°C, mutant lacking the mini chain Homo sapiens
3.4.22.16 0.00000052
-
bovine stefin B pH 6.5, 25°C, native enzyme Homo sapiens