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Literature summary extracted from

  • Lee, S.S.; Yu, S.; Withers, S.G.
    Detailed dissection of a new mechanism for glycoside cleavage: alpha-1,4-glucan lyase (2003), Biochemistry, 42, 13081-13090.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.13 deoxynojirimycin
-
 Gracilariopsis sp.
4.2.2.13 hydroximinogluconolactam poor Gracilariopsis sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.13 0.59
-
 3,4-dinitrophenyl alpha-D-glucopyranoside pH 6.0, 30°C Gracilariopsis sp.
4.2.2.13 0.77
-
 2,4,6-trichlorophenyl alpha-D-glucopyranoside pH 6.0, 30°C Gracilariopsis sp.
4.2.2.13 2.1
-
 4-nitrophenyl alpha-D-glucopyranoside pH 6.0, 30°C Gracilariopsis sp.
4.2.2.13 2.1
-
 4-chlorophenyl alpha-D-glucopyranoside pH 6.0, 30°C Gracilariopsis sp.
4.2.2.13 2.2
-
 3-nitrophenyl alpha-D-glucopyranoside pH 6.0, 30°C Gracilariopsis sp.
4.2.2.13 2.7
-
 4-chloro-2-nitrophenyl alpha-D-glucopyranoside pH 6.0, 30°C Gracilariopsis sp.
4.2.2.13 3.1
-
 3,5-dichlorophenyl alpha-D-glucopyranoside pH 6.0, 30°C Gracilariopsis sp.
4.2.2.13 3.8
-
 2,4-dinitrophenyl alpha-D-glucopyranoside pH 6.0, 30°C Gracilariopsis sp.
4.2.2.13 4.4
-
 phenyl alpha-D-glucopyranoside pH 6.0, 30°C Gracilariopsis sp.
4.2.2.13 9.9
-
 2,5-dinitrophenyl alpha-D-glucopyranoside pH 6.0, 30°C Gracilariopsis sp.
4.2.2.13 10.7
-
 5-fluoro-alpha-D-glucopyranosyl fluoride pH 6.0, 30°C Gracilariopsis sp.
4.2.2.13 11
-
 2-nitrophenyl alpha-D-glucopyranoside pH 6.0, 30°C Gracilariopsis sp.
4.2.2.13 27.9
-
 alpha-D-glucopyranosyl fluoride pH 6.0, 30°C Gracilariopsis sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.13 Gracilariopsis sp.
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.13 2,4,6-trichlorophenyl alpha-D-glucopyranoside
-
 Gracilariopsis sp. ?
-
 ?
4.2.2.13 2,4-dinitrophenyl alpha-D-glucopyranoside
-
 Gracilariopsis sp. ?
-
 ?
4.2.2.13 2,5-dinitrophenyl alpha-D-glucopyranoside
-
 Gracilariopsis sp. ?
-
 ?
4.2.2.13 2-nitrophenyl alpha-D-glucopyranoside
-
 Gracilariopsis sp. ?
-
 ?
4.2.2.13 3,4-dinitrophenyl alpha-D-glucopyranoside
-
 Gracilariopsis sp. ?
-
 ?
4.2.2.13 3,5-dichlorophenyl alpha-D-glucopyranoside
-
 Gracilariopsis sp. ?
-
 ?
4.2.2.13 3-nitrophenyl alpha-D-glucopyranoside
-
 Gracilariopsis sp. ?
-
 ?
4.2.2.13 4-chloro-2-nitrophenyl alpha-D-glucopyranoside
-
 Gracilariopsis sp. ?
-
 ?
4.2.2.13 4-chlorophenyl alpha-D-glucopyranoside
-
 Gracilariopsis sp. ?
-
 ?
4.2.2.13 4-nitrophenyl alpha-D-glucopyranoside
-
 Gracilariopsis sp. ?
-
 ?
4.2.2.13 5-fluoro-alpha-D-glucopyranosyl fluoride
-
 Gracilariopsis sp. ?
-
 ?
4.2.2.13 alpha-D-glucopyranosyl fluoride
-
 Gracilariopsis sp. ?
-
 ?
4.2.2.13 phenyl alpha-D-glucopyranoside
-
 Gracilariopsis sp. ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.2.13 0.11
-
 phenyl alpha-D-glucopyranoside pH 6.0, 30°C Gracilariopsis sp.
4.2.2.13 0.131
-
 5-fluoro-alpha-D-glucopyranosyl fluoride pH 6.0, 30°C Gracilariopsis sp.
4.2.2.13 0.4
-
 4-nitrophenyl alpha-D-glucopyranoside pH 6.0, 30°C Gracilariopsis sp.
4.2.2.13 0.44
-
 4-chlorophenyl alpha-D-glucopyranoside pH 6.0, 30°C Gracilariopsis sp.
4.2.2.13 0.66
-
 3-nitrophenyl alpha-D-glucopyranoside pH 6.0, 30°C Gracilariopsis sp.
4.2.2.13 1
-
 2,4,6-trichlorophenyl alpha-D-glucopyranoside pH 6.0, 30°C Gracilariopsis sp.
4.2.2.13 1.5
-
 3,5-dichlorophenyl alpha-D-glucopyranoside pH 6.0, 30°C Gracilariopsis sp.
4.2.2.13 1.7
-
 3,4-dinitrophenyl alpha-D-glucopyranoside pH 6.0, 30°C Gracilariopsis sp.
4.2.2.13 5.3
-
 2-nitrophenyl alpha-D-glucopyranoside pH 6.0, 30°C Gracilariopsis sp.
4.2.2.13 6.08
-
 3-nitrophenyl alpha-D-glucopyranoside pH 6.0, 30°C Gracilariopsis sp.
4.2.2.13 6.3
-
 4-chloro-2-nitrophenyl alpha-D-glucopyranoside pH 6.0, 30°C Gracilariopsis sp.
4.2.2.13 11
-
 2,5-dinitrophenyl alpha-D-glucopyranoside pH 6.0, 30°C Gracilariopsis sp.
4.2.2.13 27
-
 2,4-dinitrophenyl alpha-D-glucopyranoside pH 6.0, 30°C Gracilariopsis sp.
4.2.2.13 505
-
 alpha-D-glucopyranosyl fluoride pH 6.0, 30°C Gracilariopsis sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.13 4.5
-
-
 Gracilariopsis sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.13 3 6.5 pH 3: about 50% of maximal activity, pH 6.5: about 60% of maximal activity Gracilariopsis sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.2.13 0.00013
-
 deoxynojirimycin pH 6.0, 30°C Gracilariopsis sp.
4.2.2.13 1.33
-
 hydroximinogluconolactam pH 6.0, 30°C Gracilariopsis sp.