Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Christenson, S.D.; Wu, W.; Spies, M.A.; Shen, B.; Toney, M.D.
    Kinetic analysis of the 4-methylideneimidazole-5-one-containing tyrosine aminomutase in enediyne antitumor antibiotic C-1027 biosynthesis (2003), Biochemistry, 42, 12708-12718.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.3.6 expression in Escherichia coli Streptomyces globisporus

Protein Variants

EC Number Protein Variants Comment Organism
5.4.3.6 S153A 1.5% of wild-type activity Streptomyces globisporus
5.4.3.6 S153C 5% of wild-type activity Streptomyces globisporus

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.4.3.6 Cd2+ weak inhibition Streptomyces globisporus
5.4.3.6 Cu2+ strong inhibition Streptomyces globisporus
5.4.3.6 Zn2+ weak inhibition Streptomyces globisporus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.3.6 0.0023
-
 L-tyrosine lyase activity Streptomyces globisporus
5.4.3.6 0.028
-
 L-tyrosine
-
 Streptomyces globisporus
5.4.3.6 1.54
-
 L-3,4-dihydroxyphenylalanine
-
 Streptomyces globisporus
5.4.3.6 6.1
-
 L-3-chlorotyrosine
-
 Streptomyces globisporus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.3.6 58100
-
 x * 58100, SDS-PAGE Streptomyces globisporus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.3.6 L-tyrosine Streptomyces globisporus involved in the biosynthesis of the enediyne antitumor antibiotic C-1027 3-amino-3-(4-hydroxyphenyl)propanoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.4.3.6 Streptomyces globisporus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.3.6 recombinant tyrosine aminomutase Streptomyces globisporus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.3.6 (3S)-amino-3-(4-hydroxyphenyl)propanoate beta-tyrosine racemase activity Streptomyces globisporus (3R)-amino-3-(4-hydroxyphenyl)propanoate
-
 r
5.4.3.6 L-3,4-dihydroxyphenylalanine
-
 Streptomyces globisporus 3-amino-3-(3,4-dihydroxyphenyl)propanoate
-
 ?
5.4.3.6 L-3-chlorotyrosine
-
 Streptomyces globisporus 3-amino-3-(3-chloro-4-hydroxyphenyl)propanoate
-
 ?
5.4.3.6 L-tyrosine
-
 Streptomyces globisporus 3-amino-3-(4-hydroxyphenyl)propanoate
-
 r
5.4.3.6 L-tyrosine involved in the biosynthesis of the enediyne antitumor antibiotic C-1027 Streptomyces globisporus 3-amino-3-(4-hydroxyphenyl)propanoate
-
 ?
5.4.3.6 L-tyrosine ammonia lyase activity of tyrosine aminomutase Streptomyces globisporus (4-hydroxyphenyl)prop-2-enoate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
5.4.3.6 ? x * 58100, SDS-PAGE Streptomyces globisporus

Synonyms

EC Number Synonyms Comment Organism
5.4.3.6 SgcC4
-
 Streptomyces globisporus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.4.3.6 0.0012
-
 L-tyrosine lyase activity Streptomyces globisporus
5.4.3.6 0.0048
-
 L-3-chlorotyrosine
-
 Streptomyces globisporus
5.4.3.6 0.0067
-
 L-3,4-dihydroxyphenylalanine
-
 Streptomyces globisporus
5.4.3.6 0.01
-
 L-tyrosine
-
 Streptomyces globisporus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.3.6 8.8
-
-
 Streptomyces globisporus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.4.3.6 6.5 10.5 approx. 50% of maximal activity at pH 7.5 and pH 10.0, respectively Streptomyces globisporus