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Literature summary extracted from

  • Schilling, O.; Wenzel, N.; Naylor, M.; Vogel, A.; Crowder, M.; Makaroff, C.; Meyer-Klaucke, W.
    Flexible metal binding of the metallo-beta-lactamase domain: glyoxalase II incorporates iron, manganese, and zinc in vivo (2003), Biochemistry, 42, 11777-11786.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.1.2.6 R248W mutant shows enhanced zinc content and reduced iron content compared to the wild-type enzyme Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.6 Fe2+ addition to the medium slightly reduces the activity in vivo Arabidopsis thaliana
3.1.2.6 Mn2+ addition to the medium reduces the activity in vivo Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.6 0.12
-
 S-D-lactoylglutathione recombinant enzyme grown in medium supplemented with Mn2+, pH 7.2, 25°C Arabidopsis thaliana
3.1.2.6 0.22
-
 S-D-lactoylglutathione recombinant enzyme grown in medium not supplemented with metal ions or supplemented with Fe2+, pH 7.2, 25°C Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.2.6 Fe2+ 0.8 mol per mol of protein, wild-type enzyme, 0.4 mol per mol of protein, R248W mutant enzyme Arabidopsis thaliana
3.1.2.6 Mn2+ 0.1 mol per mol of protein, R248W mutant enzyme Arabidopsis thaliana
3.1.2.6 additional information enzyme possesses a binuclear active site, metal ion binding structure and kinetics, overview Arabidopsis thaliana
3.1.2.6 Zn2+ 0.4 mol per mol of protein, wild-type enzyme, 2.1 mol per mol of protein, R248W mutant enzyme Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.6 Arabidopsis thaliana O24496 recombinant enzyme expressed in Escherichia coli
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.6 additional information
-
-
 Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.6 S-D-lactoylglutathione + H2O
-
 Arabidopsis thaliana glutathione + D-lactate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.2.6 acetoacetylglutathione hydrolase
-
 Arabidopsis thaliana
3.1.2.6 Germ cell specific protein
-
 Arabidopsis thaliana
3.1.2.6 GLO II
-
 Arabidopsis thaliana
3.1.2.6 GlxII
-
 Arabidopsis thaliana
3.1.2.6 glyoxalase II
-
 Arabidopsis thaliana
3.1.2.6 hydrolase, acetoacetylglutathione
-
 Arabidopsis thaliana
3.1.2.6 hydrolase, hydroxyacylglutathione
-
 Arabidopsis thaliana
3.1.2.6 More enzyme belongs to the metallo-beta-lactamase superfamily Arabidopsis thaliana
3.1.2.6 Round spermatid protein RSP29
-
 Arabidopsis thaliana
3.1.2.6 S-2-hydroxylacylglutathione hydrolase
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.2.6 25
-
 assay at Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.2.6 168
-
 S-D-lactoylglutathione recombinant enzyme grown in medium supplemented with Mn2+, pH 7.2, 25°C Arabidopsis thaliana
3.1.2.6 359
-
 S-D-lactoylglutathione recombinant enzyme grown in medium supplemented with Fe2+, pH 7.2, 25°C Arabidopsis thaliana
3.1.2.6 408
-
 S-D-lactoylglutathione recombinant enzyme grown in medium not supplemented with metal ions, pH 7.2, 25°C Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.6 7.2
-
 assay at Arabidopsis thaliana