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Literature summary extracted from

  • Wild, M.R.; Pos, K.M.; Dimroth, P.
    Site-directed sulfhydryl labeling of the oxaloacetate decarboxylase Na+ pump of Klebsiella pneumoniae: helix VIII comprises a portion of the sodium ion channel (2003), Biochemistry, 42, 11615-11624.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.112 expressed in Escherichia coli Klebsiella pneumoniae

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.112 A383C beta subunit, very low activity, low activity after modification with 2-aminoethyl methanethiosulfonate Klebsiella pneumoniae
4.1.1.112 G377C beta subunit, no activity even after modification with 2-aminoethyl methanethiosulfonate Klebsiella pneumoniae
4.1.1.112 G380C beta subunit, very low activity, low activity after modification with 2-aminoethyl methanethiosulfonate Klebsiella pneumoniae
4.1.1.112 additional information several other mutant with reduced activity Klebsiella pneumoniae
4.1.1.112 R389C beta subunit, increased pH-optimum, reduced activity, activity and pH-optimum can be restored to wild type value by modification with 2-aminoethyl methanethiosulfonate Klebsiella pneumoniae
4.1.1.112 S382C beta subunit, no activity even after modification with 2-aminoethyl methanethiosulfonate Klebsiella pneumoniae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.1.112 membrane beta and gamma subunits are integral membrane proteins, alpha subunit is attached to the gamma subunit Klebsiella pneumoniae 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.112 Zn2+ binds to the gamma subunit Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.112 Oxaloacetate Klebsiella pneumoniae one Na+ is transported into the periplasm and one H+ is transported into the cytoplasm during the reaction Pyruvate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.112 Klebsiella pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.112 recombinant enzyme from Escherichia coli Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.112 Oxaloacetate
-
 Klebsiella pneumoniae Pyruvate + CO2
-
 ?
4.1.1.112 Oxaloacetate one Na+ is transported into the periplasm and one H+ is transported into the cytoplasm during the reaction Klebsiella pneumoniae Pyruvate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.112 trimer alpha, beta, gamma Klebsiella pneumoniae

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.112 biotin binds to the C-terminal domain of the alpha-subunit Klebsiella pneumoniae