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Literature summary extracted from

  • Glasner, M.E.; Bergman, N.H.; Bartel, D.P.
    Metal ion requirements for structure and catalysis of an RNA ligase ribozyme (2002), Biochemistry, 41, 8103-8112.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.3
-
 synthetic construct

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.5.1.3 Ba2+
-
 synthetic construct
6.5.1.3 Ca2+
-
 synthetic construct
6.5.1.3 Cd2+
-
 synthetic construct
6.5.1.3 Co(NH3)63+
-
 synthetic construct
6.5.1.3 Co2+
-
 synthetic construct
6.5.1.3 Mn2+
-
 synthetic construct
6.5.1.3 Pb2+
-
 synthetic construct
6.5.1.3 spermine
-
 synthetic construct
6.5.1.3 Sr2+
-
 synthetic construct
6.5.1.3 Zn2+
-
 synthetic construct

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.3 Mg2+ requires metal ions for structure and catalysis synthetic construct
6.5.1.3 Mn2+ only marginal activity synthetic construct
6.5.1.3 additional information no detectable activity with Ca2+, Sr2+, Ba2+, Zn2+, Co2+, Cd2+, Pb2+, Co(NH3)6 3+, or spermine synthetic construct

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.5.1.3 ATP + (ribonucleotide)n + (ribonucleotide)m synthetic construct
-
 AMP + diphosphate + (ribonucleotide)n+m
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.3 synthetic construct
-
 isolated from random sequence
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.3
-
 synthetic construct

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.3 ATP + (ribonucleotide)n + (ribonucleotide)m
-
 synthetic construct AMP + diphosphate + (ribonucleotide)n+m
-
 ?
6.5.1.3 ATP + 5'-aaaCCAGUC RNA-DNA hybrid as substrate, chemical step is represented as irreversible, because reverse reaction, pyrophosphorolysis with formation of a triphosphate is 10000000-times slower than the forward reaction synthetic construct AMP + diphosphate + ?
-
 ir

Synonyms

EC Number Synonyms Comment Organism
6.5.1.3 class I ligase
-
 synthetic construct
6.5.1.3 RNA ligase ribozyme
-
 synthetic construct

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.5.1.3 5.7 7
-
 synthetic construct