EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.3.25 | expressed in Escherichia coli | Mycobacterium tuberculosis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.3.25 | D104N | activity almost completely abolished | Mycobacterium tuberculosis |
3.1.3.25 | D107N | activity almost completely abolished | Mycobacterium tuberculosis |
3.1.3.25 | D235N | activity almost completely abolished | Mycobacterium tuberculosis |
3.1.3.25 | E83D | activity almost completely abolished | Mycobacterium tuberculosis |
3.1.3.25 | L81A | 10fold more resistant towards Li+ inhibition compared to wild type enzyme | Mycobacterium tuberculosis |
3.1.3.25 | W234L | activity almost completely abolished | Mycobacterium tuberculosis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.25 | Be2+ | 50% inhibition at 0.09 mM | Mycobacterium tuberculosis | |
3.1.3.25 | Ca2+ | 50% inhibition at 0.4 mM | Mycobacterium tuberculosis | |
3.1.3.25 | Co2+ | 50% inhibition at 0.7 mM | Mycobacterium tuberculosis | |
3.1.3.25 | Cr2+ | 50% inhibition at 3 mM | Mycobacterium tuberculosis | |
3.1.3.25 | Cu2+ | 50% inhibition at 1 mM | Mycobacterium tuberculosis | |
3.1.3.25 | Li+ | 50% inhibition at 0.9 mM | Mycobacterium tuberculosis | |
3.1.3.25 | Mn2+ | 50% inhibition at 0.8 mM | Mycobacterium tuberculosis | |
3.1.3.25 | Ni2+ | 50% inhibition at 30 mM | Mycobacterium tuberculosis | |
3.1.3.25 | Zn2+ | 50% inhibition at 0.005 mM | Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.25 | 0.177 | - |
myo-inositol 1-phosphate | pH 8.5, 37°C | Mycobacterium tuberculosis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.25 | Co2+ | 30% of the activity with Mg2+ | Mycobacterium tuberculosis | |
3.1.3.25 | Fe2+ | 30% of the activity with Mg2+ | Mycobacterium tuberculosis | |
3.1.3.25 | Mg2+ | required, highest activity 6-100 mM Mg2+, inhibitory above 100 mM | Mycobacterium tuberculosis | |
3.1.3.25 | Mn2+ | 13% of the activity with Mn2+ | Mycobacterium tuberculosis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.3.25 | 30000 | - |
calculated from amino acid sequence | Mycobacterium tuberculosis |
3.1.3.25 | 36000 | - |
3 * 36000, SDs-PAGE | Mycobacterium tuberculosis |
3.1.3.25 | 93000 | - |
native PAGE | Mycobacterium tuberculosis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.25 | myo-inositol 1-phosphate + H2O | Mycobacterium tuberculosis | synthesis of myo-inositol, that is a key substrate for synthesis of phosphatidylinositol | myo-inositol + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.25 | Mycobacterium tuberculosis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.3.25 | recombinant protein from Escherichia coli using His-tag | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.25 | 2'-AMP + H2O | - |
Mycobacterium tuberculosis | adenosine + phosphate | - |
? | |
3.1.3.25 | glucitol 6-phosphate + H2O | - |
Mycobacterium tuberculosis | glucitol + phosphate | - |
? | |
3.1.3.25 | glycerol 2-phosphate + H2O | - |
Mycobacterium tuberculosis | glycerol + phosphate | - |
? | |
3.1.3.25 | mannitol 1-phosphate + H2O | - |
Mycobacterium tuberculosis | mannitol + phosphate | - |
? | |
3.1.3.25 | myo-inositol 1-phosphate + H2O | - |
Mycobacterium tuberculosis | myo-inositol + phosphate | - |
? | |
3.1.3.25 | myo-inositol 1-phosphate + H2O | synthesis of myo-inositol, that is a key substrate for synthesis of phosphatidylinositol | Mycobacterium tuberculosis | myo-inositol + phosphate | - |
? | |
3.1.3.25 | myo-inositol 2-phosphate + H2O | - |
Mycobacterium tuberculosis | myo-inositol + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.3.25 | trimer | 3 * 36000, SDs-PAGE | Mycobacterium tuberculosis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.25 | 80 | - |
- |
Mycobacterium tuberculosis |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.25 | 40 | 100 | - |
Mycobacterium tuberculosis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.25 | 70 | - |
50% of activity lost within 1 min, Mg2+ stabilizes activity for short periods | Mycobacterium tuberculosis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.25 | 3.6 | - |
myo-inositol 1-phosphate | pH 8.5, 37°C | Mycobacterium tuberculosis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.25 | 8.5 | - |
- |
Mycobacterium tuberculosis |