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Literature summary extracted from

  • Andreassi, J.L.2nd.; Moran, R.G.
    Mouse folylpoly-gamma-glutamate synthetase isoforms respond differently to feedback inhibition by folylpolyglutamate cofactors (2002), Biochemistry, 41, 226-235.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.17 expression of the liver or L1210 isoforms in Sf9 insect cells Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.17 (6R)-10-formyl-5,6,7,8-tetrahydropteroylpentaglutamate
-
 Mus musculus
6.3.2.17 (6S)-5,10-methylene-5,6,7,8-tetrahydropteroylpentaglutamate
-
 Mus musculus
6.3.2.17 (6S)-5,6,7,8-tetrahydropteroylpentaglutamate
-
 Mus musculus
6.3.2.17 (6S)-5-formyl-5,6,7,8-tetrahydropteroylpentaglutamate
-
 Mus musculus
6.3.2.17 (6S)-5-methyl-5,6,7,8-tetrahydropteroylpentaglutamate
-
 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.17 0.0012
-
 5,10-dideazatetrahydrofolic acid pH 8.9, 37ºC, L1210 isoform Mus musculus
6.3.2.17 0.0014
-
 (6S)-5,6,7,8-tetrahydropteroylglutamate pH 8.9, 37ºC, L1210 isoform Mus musculus
6.3.2.17 0.002
-
 (6R)-10-formyl-5,6,7,8-tetrahydropteroylglutamate pH 8.9, 37ºC, L1210 isoform Mus musculus
6.3.2.17 0.0029
-
 (6R)-10-formyl-5,6,7,8-tetrahydropteroylglutamate pH 8.9, 37ºC, liver isoform Mus musculus
6.3.2.17 0.0063
-
 (6S)-5,6,7,8-tetrahydropteroylglutamate pH 8.9, 37ºC, liver isoform Mus musculus
6.3.2.17 0.0076
-
 5,10-dideazatetrahydrofolic acid pH 8.9, 37ºC, liver isoform Mus musculus
6.3.2.17 0.0154
-
 aminopterin pH 8.9, 37ºC, L1210 isoform Mus musculus
6.3.2.17 0.0177
-
 aminopterin pH 8.9, 37ºC, liver isoform Mus musculus
6.3.2.17 0.054
-
 (6S)-5-methyl-5,6,7,8-tetrahydropteroylglutamate pH 8.9, 37ºC, L1210 isoform Mus musculus
6.3.2.17 0.064
-
 (6S)-5-methyl-5,6,7,8-tetrahydropteroylglutamate pH 8.9, 37ºC, liver isoform Mus musculus
6.3.2.17 0.075
-
 (6S)-5-formyl-5,6,7,8-tetrahydropteroylglutamate pH 8.9, 37ºC, L1210 isoform Mus musculus
6.3.2.17 0.122
-
 (6S)-5-formyl-5,6,7,8-tetrahydropteroylglutamate pH 8.9, 37ºC, liver isoform Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.2.17 cytosol
-
 Mus musculus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.17 60600
-
 L1210 isoform Mus musculus
6.3.2.17 62000
-
 liver isoform Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.17 ATP + tetrahydrofolate-Glu + L-glutamate Mus musculus
-
 ADP + phosphate + tetrahydrofolyl-Glu2
-
 ?
6.3.2.17 additional information Mus musculus the enzyme metabolizes tetrahydrofolates to long chain polyglutamates within the cytosol or mitochondria of the cells as a mechanism to trap them within the cell ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.17 Mus musculus
-
 mouse
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.17 ammonium sulfate precipitation, gel filtration and ion-exchange chromatography Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.2.17 L-1210 cell leukemia line Mus musculus
-
6.3.2.17 liver
-
 Mus musculus
-
6.3.2.17 additional information the isozymes from the two tissues differ by 18 amino acids at their amino terminus Mus musculus
-

Storage Stability

EC Number Storage Stability Organism
6.3.2.17 -20ºC, buffered 50% glycerol, quite stable Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.17 ATP + (6R)-10-formyl-5,6,7,8-tetrahydropteroylglutamate + L-glutamate
-
 Mus musculus ADP + phosphate + (6R)-10-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
 ?
6.3.2.17 ATP + (6S)-5,6,7,8-tetrahydropteroylglutamate + L-glutamate
-
 Mus musculus ADP + phosphate + (6S)-5,6,7,8-tetrahydropteroyl-Glu2
-
 ?
6.3.2.17 ATP + (6S)-5-formyl-5,6,7,8-tetrahydropteroylglutamate + L-glutamate poor substrate Mus musculus ADP + phosphate + (6S)-5-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
 ?
6.3.2.17 ATP + (6S)-5-methyl-5,6,7,8-tetrahydropteroylglutamate + L-glutamate poor substrate Mus musculus ADP + phosphate + (6S)-5-methyl-5,6,7,8-tetrahydropteroyl-Glu2
-
 ?
6.3.2.17 ATP + (6S)-5-methyl-5,6,7,8-tetrahydropteroylglutamate + L-glutamate
-
 Mus musculus ADP + phosphate + (6S)-5-methyl-5,6,7,8-tetrahydropteroyldiglutamate
-
 ?
6.3.2.17 ATP + 5,10-dideazatetrahydrofolic acid + L-glutamate
-
 Mus musculus ADP + 5,10-dideazatetrahydrofolyl-L-Glu + phosphate
-
 ?
6.3.2.17 ATP + 5-methyl-5,6,7,8-tetrahydropteroylglutamate + L-glutamate
-
 Mus musculus ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-Glu2
-
 ?
6.3.2.17 ATP + aminopterin + L-glutamate
-
 Mus musculus ?
-
 ?
6.3.2.17 ATP + tetrahydrofolate-Glu + L-glutamate
-
 Mus musculus ADP + phosphate + tetrahydrofolyl-Glu2
-
 ?
6.3.2.17 additional information alterations in the amino terminal structure of the enzyme may influence its catalytic and/or substrate binding properties Mus musculus ?
-
 ?
6.3.2.17 additional information the enzyme metabolizes tetrahydrofolates to long chain polyglutamates within the cytosol or mitochondria of the cells as a mechanism to trap them within the cell Mus musculus ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.2.17 0.01
-
 5,10-dideazatetrahydrofolic acid L1210 isoform Mus musculus
6.3.2.17 0.073
-
 (6S)-5-formyl-5,6,7,8-tetrahydropteroylglutamate L1210 isoform Mus musculus
6.3.2.17 0.095
-
 (6R)-10-formyl-5,6,7,8-tetrahydropteroylglutamate L1210 isoform Mus musculus
6.3.2.17 0.11
-
 5-methyl-5,6,7,8-tetrahydropteroylglutamate L1210 isoform Mus musculus
6.3.2.17 0.115
-
 (6S)-5-formyl-5,6,7,8-tetrahydropteroylglutamate liver isoform Mus musculus
6.3.2.17 0.116
-
 (6S)-5,6,7,8-tetrahydropteroylglutamate L1210 isoform Mus musculus
6.3.2.17 0.145
-
 5,10-dideazatetrahydrofolic acid liver isoform Mus musculus
6.3.2.17 0.165
-
 aminopterin L1210 isoform Mus musculus
6.3.2.17 0.212
-
 (6R)-10-formyl-5,6,7,8-tetrahydropteroylglutamate liver isoform Mus musculus
6.3.2.17 0.215
-
 (6S)-5,6,7,8-tetrahydropteroylglutamate liver isoform Mus musculus
6.3.2.17 0.22
-
 aminopterin liver isoform Mus musculus
6.3.2.17 0.22
-
 5-methyl-5,6,7,8-tetrahydropteroylglutamate liver isoform Mus musculus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.3.2.17 0.0017
-
 (6S)-5,10-methylene-5,6,7,8-tetrahydropteroylpentaglutamate Ki slope, L1210 isoform Mus musculus
6.3.2.17 0.0023
-
 (6R)-10-formyl-5,6,7,8-tetrahydropteroylpentaglutamate Ki slope, L1210 isoform Mus musculus
6.3.2.17 0.0024
-
 (6R)-10-formyl-5,6,7,8-tetrahydropteroylpentaglutamate Ki slope, liver isoform Mus musculus
6.3.2.17 0.0037
-
 (6S)-5,6,7,8-tetrahydropteroylpentaglutamate Ki slope, L1210 isoform Mus musculus
6.3.2.17 0.0042
-
 (6R)-10-formyl-5,6,7,8-tetrahydropteroylpentaglutamate Ki intercept, L1210 isoform Mus musculus
6.3.2.17 0.0052
-
 (6R)-10-formyl-5,6,7,8-tetrahydropteroylpentaglutamate Ki intercept, liver isoform Mus musculus
6.3.2.17 0.0083
-
 (6S)-5,6,7,8-tetrahydropteroylpentaglutamate Ki intercept, L1210 isoform Mus musculus
6.3.2.17 0.0083
-
 (6S)-5,10-methylene-5,6,7,8-tetrahydropteroylpentaglutamate Ki intercept, L1210 isoform Mus musculus
6.3.2.17 0.02
-
 (6S)-5-methyl-5,6,7,8-tetrahydropteroylpentaglutamate Ki slope, L1210 isoform Mus musculus
6.3.2.17 0.021
-
 (6S)-5,6,7,8-tetrahydropteroylpentaglutamate Ki slope, liver isoform Mus musculus
6.3.2.17 0.022
-
 (6S)-5,10-methylene-5,6,7,8-tetrahydropteroylpentaglutamate Ki slope, liver isoform Mus musculus
6.3.2.17 0.043
-
 (6S)-5,10-methylene-5,6,7,8-tetrahydropteroylpentaglutamate Ki intercept, liver isoform Mus musculus
6.3.2.17 0.054
-
 (6S)-5,6,7,8-tetrahydropteroylpentaglutamate Ki intercept, liver isoform Mus musculus
6.3.2.17 0.055
-
 (6S)-5-methyl-5,6,7,8-tetrahydropteroylpentaglutamate Ki slope, liver isoform Mus musculus
6.3.2.17 0.066
-
 (6S)-5-formyl-5,6,7,8-tetrahydropteroylpentaglutamate Ki slope, L1210 isoform Mus musculus
6.3.2.17 0.084
-
 (6S)-5-formyl-5,6,7,8-tetrahydropteroylpentaglutamate Ki slope, liver isoform Mus musculus