EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
6.3.5.5 | additional information | A series of mutants created and probes the direct transport of carbamate through the carbamate tunnel, the partial reactions of enzyme have not been significantly impaired by these mutations, and thus, the catalytic machinery at the individual active site has not been functionally pertured | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.5.5 | additional information | - |
additional information | comparison of KM of partial reactions of wild-type and mutant enzymes | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.3.5.5 | 42000 | - |
alpha,beta, 1 * 42000 + 1 * 118000, the ammonia tunnel is responsible for the migration of ammonia, that is produced at the active site of the small subunit and migrates to the large subunit, the carbamate tunnel connects the two active sites contained within the carboxy phosphate and carbamoyl phosphate domains of the large subunit | Escherichia coli |
6.3.5.5 | 118000 | - |
alpha,beta, 1 * 42000 + 1 * 118000, the ammonia tunnel is responsible for the migration of ammonia, that is produced at the active site of the small subunit and migrates to the large subunit, the carbamate tunnel connects the two active sites contained within the carboxy phosphate and carbamoyl phosphate domains of the large subunit | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.5.5 | 2 ATP + L-Gln + HCO3- | Escherichia coli | - |
2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.5.5 | Escherichia coli | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
6.3.5.5 | 2 ATP + L-glutamine + hydrogencarbonate + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate | catalytic mechanism | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.5.5 | 2 ATP + L-Gln + HCO3- | - |
Escherichia coli | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.3.5.5 | heterodimer | alpha,beta, 1 * 42000 + 1 * 118000, the ammonia tunnel is responsible for the migration of ammonia, that is produced at the active site of the small subunit and migrates to the large subunit, the carbamate tunnel connects the two active sites contained within the carboxy phosphate and carbamoyl phosphate domains of the large subunit | Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.5.5 | additional information | - |
additional information | comparison of kcat of partial reactions of wild-type and mutant enzymes | Escherichia coli |