EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.9 | Thr-tRNAValC76 | - |
Escherichia coli | |
6.1.1.9 | tRNAVal(-ACCA) | - |
Escherichia coli | |
6.1.1.9 | tRNAVal(-CCA) | - |
Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.9 | 0.0395 | - |
L-valine | pH 7.5, 37°C | Escherichia coli | |
6.1.1.9 | 1.9 | - |
L-isoleucine | pH 7.5, 37°C | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.9 | ATP + L-valine + tRNAVal | Escherichia coli | - |
AMP + diphosphate + L-valyl-tRNAVal | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.1.1.9 | Escherichia coli | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
6.1.1.9 | ATP + L-valine + tRNAVal = AMP + diphosphate + L-valyl-tRNAVal | tRNAVal 3'-end, and a purine at position 76, is crucial for substrate editing mechanism | Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.1.1.9 | additional information | - |
- |
Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.9 | ATP + L-isoleucine + tRNAVal | very low activity | Escherichia coli | AMP + diphosphate + L-isoleucyl-tRNAVal | - |
? | |
6.1.1.9 | ATP + L-valine + tRNAVal | - |
Escherichia coli | AMP + diphosphate + L-valyl-tRNAVal | - |
? | |
6.1.1.9 | ATP + L-valine + tRNAVal | enzyme contains 2 tRNA binding sites involved in aminoacetylation and editing reactions, misacetylated tRNAVal is edited by the enzyme to avoid accumulation, the 3'-end of the tRNA is involved | Escherichia coli | AMP + diphosphate + L-valyl-tRNAVal | - |
? | |
6.1.1.9 | additional information | tRNAVal variants in position 76, i.e. U76, C76, G76 activate the editing activity, misactivation of threonine, alanine, serine, cysteine, alpha-aminobutyrate, and to a low extent of norvaline activates the editing reaction at different rates, overview, the enzyme is unable to deacylate misacylated tRNAVal terminating in 3'-pyrimidines but does deacylate mischarged tRNAVal terminating in adenosine or guanosine, no misactivation of methionine, leucine, glycine, glutamic acid, lysine, tyrosine, and phenylalanine | Escherichia coli | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.1.1.9 | G7a | - |
Escherichia coli |
6.1.1.9 | Synthetase, valyl-transfer ribonucleate | - |
Escherichia coli |
6.1.1.9 | Valine transfer ribonucleate ligase | - |
Escherichia coli |
6.1.1.9 | Valine translase | - |
Escherichia coli |
6.1.1.9 | Valine--tRNA ligase | - |
Escherichia coli |
6.1.1.9 | ValRS | - |
Escherichia coli |
6.1.1.9 | Valyl transfer ribonucleic acid synthetase | - |
Escherichia coli |
6.1.1.9 | Valyl-transfer ribonucleate synthetase | - |
Escherichia coli |
6.1.1.9 | Valyl-transfer RNA synthetase | - |
Escherichia coli |
6.1.1.9 | Valyl-tRNA ligase | - |
Escherichia coli |
6.1.1.9 | Valyl-tRNA synthetase | - |
Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.1.1.9 | 37 | - |
aminoacylation and deacylation assay at | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.1.1.9 | 7.5 | - |
aminoacylation and deacylation assay at | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.9 | ATP | - |
Escherichia coli |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.9 | additional information | - |
additional information | inhibition kinetics | Escherichia coli | |
6.1.1.9 | 0.0085 | - |
Thr-tRNAValC76 | - |
Escherichia coli | |
6.1.1.9 | 0.02 | - |
tRNAVal(-ACCA) | pH 7.5, 37°C | Escherichia coli | |
6.1.1.9 | 0.029 | - |
tRNAVal(-CCA) | pH 7.5, 37°C | Escherichia coli |