Literature summary extracted from

Yu, B.; Thompson, G.D.; Yip, P.; Howell, P.L.; Davidson, A.R.
Mechanisms for intragenic complementation at the human argininosuccinate lyase locus (2001), Biochemistry, 40, 15581-15590.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
4.3.2.1	expression in Escherichia coli and COS-7 cells	Homo sapiens

Protein Variants

EC Number	Protein Variants	Comment	Organism
4.3.2.1	A398D	no activity, 15.55 of wild-type activity if coexpressed with Q286R	Homo sapiens
4.3.2.1	D87G	no activity, 35.2% of wild-type activity if coexpressed with Q286R	Homo sapiens
4.3.2.1	M360T	no actiivty, 10% of wild-type activity if coexpressed with Q286R	Homo sapiens
4.3.2.1	Q286R	3% of wild-type activity	Homo sapiens

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
4.3.2.1	0.12	-	N,N'-bis(tert-butoxycarbonyl)-5-[(3-aminopropyl)amino]pentan-1-ol	-	Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
4.3.2.1	(Nomega-L-arginino)succinate	Homo sapiens	-	fumarate + L-arginine	-	r

Organism

EC Number	Organism	UniProt	Comment	Textmining
4.3.2.1	Homo sapiens	-	ASL deficiency results in the autosomal, recessive disorder argininosuccinic aciduria	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
4.3.2.1	recombinant ASL	Homo sapiens

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4.3.2.1	(Nomega-L-arginino)succinate	-	Homo sapiens	fumarate + L-arginine	-	?
4.3.2.1	(Nomega-L-arginino)succinate	-	Homo sapiens	fumarate + L-arginine	-	r
4.3.2.1	N,N'-bis(tert-butoxycarbonyl)-5-[(3-aminopropyl)amino]pentan-1-ol	-	Homo sapiens	?	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
4.3.2.1	ASL	-	Homo sapiens

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
4.3.2.1	34.5	-	N,N'-bis(tert-butoxycarbonyl)-5-[(3-aminopropyl)amino]pentan-1-ol	-	Homo sapiens

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Release 2023.1 (January 2023)