Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zheng, R.; Blanchard, J.S.
    Steady-state and pre-steady-state kinetic analysis of Mycobacterium tuberculosis pantothenate synthetase (2001), Biochemistry, 40, 12904-12912.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.1 expression in Escherichia coli Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.1 pantothenate product inhibition Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.1 0.13
-
 D-pantoate 25°C, pH 7.8 Mycobacterium tuberculosis
6.3.2.1 0.8
-
 beta-Alanine 25°C, pH 7.8 Mycobacterium tuberculosis
6.3.2.1 2.6
-
 ATP 25°C, pH 7.8 Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.1 Co2+ maximal activity at 10 mM, slight activation Mycobacterium tuberculosis
6.3.2.1 Mg2+ maximal activity at 10 mM, very strong activation Mycobacterium tuberculosis
6.3.2.1 Mn2+ maximal activity at 10 mM, very strong activation Mycobacterium tuberculosis
6.3.2.1 Ni2+ maximal activity at approx. 3 mM, slight activation Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.1 32677
-
 2 * 32677, deduced from nucleotide sequence Mycobacterium tuberculosis
6.3.2.1 33000
-
 2 * 33000, SDS-PAGE Mycobacterium tuberculosis
6.3.2.1 65000
-
 gel filtration Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.1 ATP + (R)-pantoate + beta-alanine Mycobacterium tuberculosis last step in pantothenate biosynthesis pathway AMP + diphosphate + (R)-pantothenate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.1 Mycobacterium tuberculosis
-
 PanC is essential for growth
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.1 recombinant PanC Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.1 ATP + (R)-pantoate + beta-alanine
-
 Mycobacterium tuberculosis AMP + diphosphate + (R)-pantothenate
-
 ?
6.3.2.1 ATP + (R)-pantoate + beta-alanine last step in pantothenate biosynthesis pathway Mycobacterium tuberculosis AMP + diphosphate + (R)-pantothenate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.2.1 dimer 2 * 33000, SDS-PAGE Mycobacterium tuberculosis
6.3.2.1 dimer 2 * 32677, deduced from nucleotide sequence Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
6.3.2.1 PanC
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.2.1 3.4
-
 beta-Alanine 25°C, pH 7.8 Mycobacterium tuberculosis
6.3.2.1 3.4
-
 D-pantoate 25°C, pH 7.8 Mycobacterium tuberculosis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.3.2.1 4.5
-
 pantothenate 25°C, pH 7.8, vs. beta-alanine Mycobacterium tuberculosis
6.3.2.1 5.7
-
 pantothenate 25°C, pH 7.8, vs. D-pantoate Mycobacterium tuberculosis
6.3.2.1 7.6
-
 pantothenate 25°C, pH 7.8, vs. ATP Mycobacterium tuberculosis