BRENDA - Enzyme Database

Membrane lipids have multiple effects on interfacial catalysis by a phosphatidic acid-preferring phospholipase A1 from bovine testis

Lin, Q.; Higgs, H.N.; Glomset, J.A.; Biochemistry 39, 9335-9344 (2000)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
3.1.1.32
membrane lipids increase stability
Bos taurus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.32
cytosol
-
Bos taurus
5829
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.1.32
phosphatidic acid + H2O
Bos taurus
-
2-acylglycerophosphatidic acid + a carboxylate
-
Bos taurus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.32
Bos taurus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.1.32
Superdex 200, partially purified
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.32
testis
-
Bos taurus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.32
phosphatidic acid + H2O
-
650008
Bos taurus
2-acylglycerophosphatidic acid + a carboxylate
-
650008
Bos taurus
?
3.1.1.32
phosphatidic acid + H2O
the catalytic preference for phosphatidic acid increases in the presence of membranes containing cholesterol, diacylglycerol, sn-1-stearoyl-2-arachidonoylphosphatidylethanolamine instead of sn-1-palmitoyl-2-oleeoylphosphatidylethanolamine and 10 mol% phosphatidic acid per 100 mol% total phosphoglycerides
650008
Bos taurus
2-acylglycerophosphatidic acid + a carboxylate
-
650008
Bos taurus
?
3.1.1.32
phosphatidylcholine + H2O
18fold lower initial rate than with phosphatidic acid
650008
Bos taurus
2-acylglycerophosphocholine + a carboxylate
-
650008
Bos taurus
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.1.32
37
-
75% loss of activity after 1 h, only little loss of activity in the presence of sn-1-alkyl-2-oleoyl or lysophosphatidic acid containing membranes
Bos taurus
General Stability (protein specific)
EC Number
General Stability
Organism
3.1.1.32
membrane lipids increase stability
Bos taurus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.32
cytosol
-
Bos taurus
5829
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.1.32
phosphatidic acid + H2O
Bos taurus
-
2-acylglycerophosphatidic acid + a carboxylate
-
Bos taurus
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.32
Superdex 200, partially purified
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.32
testis
-
Bos taurus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.32
phosphatidic acid + H2O
-
650008
Bos taurus
2-acylglycerophosphatidic acid + a carboxylate
-
650008
Bos taurus
?
3.1.1.32
phosphatidic acid + H2O
the catalytic preference for phosphatidic acid increases in the presence of membranes containing cholesterol, diacylglycerol, sn-1-stearoyl-2-arachidonoylphosphatidylethanolamine instead of sn-1-palmitoyl-2-oleeoylphosphatidylethanolamine and 10 mol% phosphatidic acid per 100 mol% total phosphoglycerides
650008
Bos taurus
2-acylglycerophosphatidic acid + a carboxylate
-
650008
Bos taurus
?
3.1.1.32
phosphatidylcholine + H2O
18fold lower initial rate than with phosphatidic acid
650008
Bos taurus
2-acylglycerophosphocholine + a carboxylate
-
650008
Bos taurus
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.1.32
37
-
75% loss of activity after 1 h, only little loss of activity in the presence of sn-1-alkyl-2-oleoyl or lysophosphatidic acid containing membranes
Bos taurus