Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Pleiss, J.A.; Wolfson, A.D.; Uhlenbeck, O.C.
    Mapping contacts between Escherichia coli alanyl tRNA synthetase and 2' hydroxyls using a complete tRNA molecule (2000), Biochemistry, 39, 8250-8258.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.7 0.0011
-
 tRNAAla wild-type and mutant substrate from Escherichia coli, pH 7.5, 37°C Escherichia coli
6.1.1.7 0.0019
-
 tRNAPhe(17) tRNAPhe mutant tRNAPhe(17), pH 7.5, 37°C Escherichia coli
6.1.1.7 0.0021
-
 tRNAPhe native dual specific tRNAPhe derivative substrate from Saccharomyces cerevisiae, pH 7.5, 37°C Escherichia coli
6.1.1.7 0.0031
-
 tRNAPhe(38) tRNAPhe mutant tRNAPhe(38), pH 7.5, 37°C Escherichia coli
6.1.1.7 0.017
-
 tRNAPhe(57) tRNAPhe mutant tRNAPhe(57), pH 7.5, 37°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.7 Mg2+ required Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.7 ATP + L-alanine + tRNAAla Escherichia coli
-
 AMP + diphosphate + L-alanyl-tRNAAla
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.7 Escherichia coli
-
 purified recombinant His-tagged enzyme
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.7 ATP + L-alanine + dual specific tRNAPhe effects of deoxynucleotide substitutions in the tRNAPhe substrate on the enzyme activity, overview Escherichia coli AMP + diphosphate + L-alanyl-tRNAPhe
-
 ?
6.1.1.7 ATP + L-alanine + dual specific tRNAPhe(17) site-specific modification leads to deoxynucleotide substituted yeast tRNA substrate, the backbone is interrupted on the 3'-side of nucleotide 17 Escherichia coli AMP + diphosphate + L-alanyl-tRNAPhe(17)
-
 ?
6.1.1.7 ATP + L-alanine + dual specific tRNAPhe(38) site-specific modification leads to deoxynucleotide substituted yeast tRNA substrate, the backbone is interrupted on the 3'-side of nucleotide 38 Escherichia coli AMP + diphosphate + L-alanyl-tRNAPhe(38)
-
 ?
6.1.1.7 ATP + L-alanine + dual specific tRNAPhe(57) site-specific modification leads to deoxynucleotide substituted yeast tRNA substrate, the backbone is interrupted on the 3'-side of nucleotide 57 Escherichia coli AMP + diphosphate + L-alanyl-tRNAPhe(57)
-
 ?
6.1.1.7 ATP + L-alanine + tRNAAla
-
 Escherichia coli AMP + diphosphate + L-alanyl-tRNAAla
-
 ?
6.1.1.7 ATP + L-alanine + tRNAAla wild-type and mutant Escherichia coli tRNAAla substrates, enzyme interacts with 2'-hydroxyls in the acceptor stem of the tRNA substrate Escherichia coli AMP + diphosphate + L-alanyl-tRNAAla
-
 ?
6.1.1.7 ATP + L-alanine + tRNAPhe(17)
-
 Escherichia coli ?
-
 ?
6.1.1.7 ATP + L-alanine + tRNAPhe(38)
-
 Escherichia coli ?
-
 ?
6.1.1.7 ATP + L-alanine + tRNAPhe(57)
-
 Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.7 Ala-tRNA synthetase
-
 Escherichia coli
6.1.1.7 Alanine transfer RNA synthetase
-
 Escherichia coli
6.1.1.7 Alanine translase
-
 Escherichia coli
6.1.1.7 Alanine tRNA synthetase
-
 Escherichia coli
6.1.1.7 Alanine-transfer RNA ligase
-
 Escherichia coli
6.1.1.7 alanine-tRNA ligase
-
 Escherichia coli
6.1.1.7 Alanyl-transfer ribonucleate synthetase
-
 Escherichia coli
6.1.1.7 Alanyl-transfer ribonucleic acid synthetase
-
 Escherichia coli
6.1.1.7 Alanyl-transfer RNA synthetase
-
 Escherichia coli
6.1.1.7 Alanyl-tRNA synthetase
-
 Escherichia coli
6.1.1.7 AlaRS
-
 Escherichia coli
6.1.1.7 Synthase, alanyl-transfer ribonucleate
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.7 37
-
 assay at Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.7 0.12
-
 tRNAPhe(57) tRNAPhe mutant tRNAPhe(57), pH 7.5, 37°C Escherichia coli
6.1.1.7 0.19
-
 tRNAAla mutant substrate from Escherichia coli, pH 7.5, 37°C Escherichia coli
6.1.1.7 0.25
-
 tRNAPhe native dual specific tRNAPhe substrate from Saccharomyces cerevisiae, pH 7.5, 37°C Escherichia coli
6.1.1.7 0.25
-
 tRNAPhe(17) tRNAPhe mutant tRNAPhe(17), pH 7.5, 37°C Escherichia coli
6.1.1.7 0.44
-
 tRNAAla wild-type substrate from Escherichia coli, pH 7.5, 37°C Escherichia coli
6.1.1.7 0.46
-
 tRNAPhe(38) tRNAPhe mutant tRNAPhe(38), pH 7.5, 37°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.7 7.5
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.7 ATP
-
 Escherichia coli