BRENDA - Enzyme Database

Regulation of phospholipase C-beta1 activity by phosphatidic acid

Litosch, I.; Biochemistry 39, 7736-7743 (2000)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.11
diacylglycerol
little activating effect on PLC-beta1
Bos taurus
3.1.4.11
lysophosphatidic acid
slight activation, less effective in stimulating PLC-beta1 activity than phosphatidic acid
Bos taurus
3.1.4.11
additional information
PLC-beta1 is not activated by phosphatidylserine, anionic and neutral detergents
Bos taurus
3.1.4.11
oleic acid
little activating effect on PLC-beta1
Bos taurus
3.1.4.11
phosphatidic acid
PLC-beta1 activation, Ca2+-dependent, relatively independent of acyl chain length, stimulates basal and receptor-G protein-regulated PLC-beta1 activity, activation occurs through an C-terminal-dependent and an independent mechanism
Bos taurus
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.1.4.11
additional information
C-terminal deletion mutant: 100 kDa fragment with catalytic activity
Bos taurus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.11
cholic acid
inhibits PLC-beta1 activity
Bos taurus
3.1.4.11
deoxycholate
0.1 mM, inhibits basal and phosphatidic acid-stimulated PLC-beta1 activity
Bos taurus
3.1.4.11
phosphatidylserine
30 mol%, slight PLC-beta1 inhibition
Bos taurus
3.1.4.11
Triton X-100
inhibits PLC-beta1 activity
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.11
0.45
-
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
pH 7, PLC-beta1
Bos taurus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.11
plasma membrane
PLC-beta1
Bos taurus
5886
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.11
Ca2+
requirement, phosphatidic acid reduces the Ca2+ concentration required for half maximal stimulation of PLC-beta1 activity
Bos taurus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.11
Bos taurus
-
PLC-beta1
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.4.11
PLC-beta1
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.11
brain
PLC-beta1
Bos taurus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.11
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
PLC-beta1
649997
Bos taurus
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
-
649997
Bos taurus
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.11
7
-
assay at
Bos taurus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.11
diacylglycerol
little activating effect on PLC-beta1
Bos taurus
3.1.4.11
lysophosphatidic acid
slight activation, less effective in stimulating PLC-beta1 activity than phosphatidic acid
Bos taurus
3.1.4.11
additional information
PLC-beta1 is not activated by phosphatidylserine, anionic and neutral detergents
Bos taurus
3.1.4.11
oleic acid
little activating effect on PLC-beta1
Bos taurus
3.1.4.11
phosphatidic acid
PLC-beta1 activation, Ca2+-dependent, relatively independent of acyl chain length, stimulates basal and receptor-G protein-regulated PLC-beta1 activity, activation occurs through an C-terminal-dependent and an independent mechanism
Bos taurus
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.1.4.11
additional information
C-terminal deletion mutant: 100 kDa fragment with catalytic activity
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.11
cholic acid
inhibits PLC-beta1 activity
Bos taurus
3.1.4.11
deoxycholate
0.1 mM, inhibits basal and phosphatidic acid-stimulated PLC-beta1 activity
Bos taurus
3.1.4.11
phosphatidylserine
30 mol%, slight PLC-beta1 inhibition
Bos taurus
3.1.4.11
Triton X-100
inhibits PLC-beta1 activity
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.11
0.45
-
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
pH 7, PLC-beta1
Bos taurus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.11
plasma membrane
PLC-beta1
Bos taurus
5886
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.11
Ca2+
requirement, phosphatidic acid reduces the Ca2+ concentration required for half maximal stimulation of PLC-beta1 activity
Bos taurus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.11
PLC-beta1
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.11
brain
PLC-beta1
Bos taurus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.11
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
PLC-beta1
649997
Bos taurus
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
-
649997
Bos taurus
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.11
7
-
assay at
Bos taurus