Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Litosch, I.
    Regulation of phospholipase C-beta1 activity by phosphatidic acid (2000), Biochemistry, 39, 7736-7743.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.11 diacylglycerol little activating effect on PLC-beta1 Bos taurus
3.1.4.11 lysophosphatidic acid slight activation, less effective in stimulating PLC-beta1 activity than phosphatidic acid Bos taurus
3.1.4.11 additional information PLC-beta1 is not activated by phosphatidylserine, anionic and neutral detergents Bos taurus
3.1.4.11 oleic acid little activating effect on PLC-beta1 Bos taurus
3.1.4.11 phosphatidic acid PLC-beta1 activation, Ca2+-dependent, relatively independent of acyl chain length, stimulates basal and receptor-G protein-regulated PLC-beta1 activity, activation occurs through an C-terminal-dependent and an independent mechanism Bos taurus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.11 additional information C-terminal deletion mutant: 100 kDa fragment with catalytic activity Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.11 cholic acid inhibits PLC-beta1 activity Bos taurus
3.1.4.11 deoxycholate 0.1 mM, inhibits basal and phosphatidic acid-stimulated PLC-beta1 activity Bos taurus
3.1.4.11 phosphatidylserine 30 mol%, slight PLC-beta1 inhibition Bos taurus
3.1.4.11 Triton X-100 inhibits PLC-beta1 activity Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.11 0.45
-
 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate pH 7, PLC-beta1 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.11 plasma membrane PLC-beta1 Bos taurus 5886
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.11 Ca2+ requirement, phosphatidic acid reduces the Ca2+ concentration required for half maximal stimulation of PLC-beta1 activity Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.11 Bos taurus
-
 PLC-beta1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.11 PLC-beta1 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.11 brain PLC-beta1 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.11 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O PLC-beta1 Bos taurus 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.11 7
-
 assay at Bos taurus