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Literature summary extracted from

  • Chen, J.F.; Guo, N.N.; Li, T.; Wang, E.D.; Wang, Y.L.
    CP1 domain in Escherichia coli leucyl-tRNA synthetase is crucial for its editing function (2000), Biochemistry, 39, 6726-6731.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.4 expression of the His-tagged CP1 domain mutant and isolated CP1 domain in Escherichia coli strain JM109(DE3) Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.4 additional information construction of an enzyme mutant with a duplication of the peptide fragment from Met238 to Pro368 within the CP1 domain which shows an activity reduced by 59% compared to the wild-type enzyme, and catalyzes the mischarging of tRNALeu by methionine or isoleucine due to impaired ability to edit incorrect products Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.4 0.0015
-
tRNALeu aminoacylation reaction, wild-type enzyme, pH 7.8, 37°C Escherichia coli
6.1.1.4 0.0024
-
tRNALeu aminoacylation reaction, mutant enzyme, pH 7.8, 37°C Escherichia coli
6.1.1.4 0.015
-
L-leucine aminoacylation reaction, wild-type and mutant enzyme, pH 7.8, 37°C Escherichia coli
6.1.1.4 0.052
-
L-leucine ATP-diphosphate exchange reaction, wild-type enzyme, pH 7.8, 37°C Escherichia coli
6.1.1.4 0.069
-
L-leucine ATP-diphosphate exchange reaction, mutant enzyme, pH 7.8, 37°C Escherichia coli
6.1.1.4 0.25
-
ATP aminoacylation reaction, mutant enzyme, pH 7.8, 37°C Escherichia coli
6.1.1.4 0.28
-
ATP aminoacylation reaction, wild-type enzyme, pH 7.8, 37°C Escherichia coli
6.1.1.4 2.8
-
L-isoleucine ATP-diphosphate exchange reaction, mutant enzyme, pH 7.8, 37°C Escherichia coli
6.1.1.4 3.5
-
L-isoleucine ATP-diphosphate exchange reaction, wild-type enzyme, pH 7.8, 37°C Escherichia coli
6.1.1.4 6.2
-
L-methionine ATP-diphosphate exchange reaction, mutant enzyme, pH 7.8, 37°C Escherichia coli
6.1.1.4 7.5
-
L-methionine ATP-diphosphate exchange reaction, wild-type enzyme, pH 7.8, 37°C Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.4 ATP + L-leucine + tRNALeu Escherichia coli
-
AMP + diphosphate + L-leucyl-tRNALeu
-
?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.4 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.4 recombinant His-tagged mutant and His-tagged CP1 domain expressed in Escherichia coli strain Jm109(DE3), to electrophoretic homogeneity Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.4 0.79
-
recombinant CP1 domain mutant Escherichia coli
6.1.1.4 1.5
-
about, wild-type enzyme Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.4 ATP + L-isoleucine + tRNALeu wild-type and CP1 domain mutant enzyme, the mischarged product can be edited by the wild-type enzyme, but not by a recombinant isolated CP1 domain Escherichia coli AMP + diphosphate + L-isoleucyl-tRNALeu
-
?
6.1.1.4 ATP + L-leucine + tRNALeu
-
Escherichia coli AMP + diphosphate + L-leucyl-tRNALeu
-
?
6.1.1.4 ATP + L-leucine + tRNALeu two-step reaction, the connecting peptide CP1 domain is crucial for the editing function Escherichia coli AMP + diphosphate + L-leucyl-tRNALeu
-
?
6.1.1.4 ATP + L-methionine + tRNALeu wild-type and CP1 domain mutant enzyme, the mischarged product can be edited by the wild-type enzyme, but not by a recombinant isolated CP1 domain Escherichia coli AMP + diphosphate + L-methionyl-tRNALeu
-
?
6.1.1.4 additional information the enzyme also performs the ATP-diphosphate exchange reaction Escherichia coli ?
-
?

Subunits

EC Number Subunits Comment Organism
6.1.1.4 More determination of secondary structure elements in the wild-type and mutant enzyme Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
6.1.1.4 Leucine translase
-
Escherichia coli
6.1.1.4 Leucine--tRNA ligase
-
Escherichia coli
6.1.1.4 Leucyl-transfer ribonucleate synthetase
-
Escherichia coli
6.1.1.4 Leucyl-transfer ribonucleic acid synthetase
-
Escherichia coli
6.1.1.4 Leucyl-transfer RNA synthetase
-
Escherichia coli
6.1.1.4 Leucyl-tRNA synthetase
-
Escherichia coli
6.1.1.4 LeuRS
-
Escherichia coli
6.1.1.4 Synthetase, leucyl-transfer ribonucleate
-
Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.4 37
-
assay at Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.4 1.3
-
tRNALeu mutant enzyme, pH 7.8, 37°C Escherichia coli
6.1.1.4 1.5
-
L-leucine mutant enzyme, pH 7.8, 37°C Escherichia coli
6.1.1.4 1.6
-
ATP mutant enzyme, pH 7.8, 37°C Escherichia coli
6.1.1.4 2.9
-
tRNALeu wild-type enzyme, pH 7.8, 37°C Escherichia coli
6.1.1.4 3
-
L-leucine wild-type enzyme, pH 7.8, 37°C Escherichia coli
6.1.1.4 3.6
-
ATP wild-type enzyme, pH 7.8, 37°C Escherichia coli
6.1.1.4 6.9
-
L-isoleucine ATP-diphosphate exchange reaction, mutant enzyme, pH 7.8, 37°C Escherichia coli
6.1.1.4 7.6
-
L-methionine ATP-diphosphate exchange reaction, mutant enzyme, pH 7.8, 37°C Escherichia coli
6.1.1.4 18
-
L-isoleucine ATP-diphosphate exchange reaction, wild-type enzyme, pH 7.8, 37°C Escherichia coli
6.1.1.4 19
-
L-methionine ATP-diphosphate exchange reaction, wild-type enzyme, pH 7.8, 37°C Escherichia coli
6.1.1.4 101
-
L-leucine ATP-diphosphate exchange reaction, mutant enzyme, pH 7.8, 37°C Escherichia coli
6.1.1.4 171
-
L-leucine ATP-diphosphate exchange reaction, wild-type enzyme, pH 7.8, 37°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.4 7.8
-
assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.4 ATP
-
Escherichia coli