EC Number | Cloned (Comment) | Organism |
---|---|---|
6.1.1.4 | expression of the His-tagged CP1 domain mutant and isolated CP1 domain in Escherichia coli strain JM109(DE3) | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
6.1.1.4 | additional information | construction of an enzyme mutant with a duplication of the peptide fragment from Met238 to Pro368 within the CP1 domain which shows an activity reduced by 59% compared to the wild-type enzyme, and catalyzes the mischarging of tRNALeu by methionine or isoleucine due to impaired ability to edit incorrect products | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.4 | 0.0015 | - |
tRNALeu | aminoacylation reaction, wild-type enzyme, pH 7.8, 37°C | Escherichia coli | |
6.1.1.4 | 0.0024 | - |
tRNALeu | aminoacylation reaction, mutant enzyme, pH 7.8, 37°C | Escherichia coli | |
6.1.1.4 | 0.015 | - |
L-leucine | aminoacylation reaction, wild-type and mutant enzyme, pH 7.8, 37°C | Escherichia coli | |
6.1.1.4 | 0.052 | - |
L-leucine | ATP-diphosphate exchange reaction, wild-type enzyme, pH 7.8, 37°C | Escherichia coli | |
6.1.1.4 | 0.069 | - |
L-leucine | ATP-diphosphate exchange reaction, mutant enzyme, pH 7.8, 37°C | Escherichia coli | |
6.1.1.4 | 0.25 | - |
ATP | aminoacylation reaction, mutant enzyme, pH 7.8, 37°C | Escherichia coli | |
6.1.1.4 | 0.28 | - |
ATP | aminoacylation reaction, wild-type enzyme, pH 7.8, 37°C | Escherichia coli | |
6.1.1.4 | 2.8 | - |
L-isoleucine | ATP-diphosphate exchange reaction, mutant enzyme, pH 7.8, 37°C | Escherichia coli | |
6.1.1.4 | 3.5 | - |
L-isoleucine | ATP-diphosphate exchange reaction, wild-type enzyme, pH 7.8, 37°C | Escherichia coli | |
6.1.1.4 | 6.2 | - |
L-methionine | ATP-diphosphate exchange reaction, mutant enzyme, pH 7.8, 37°C | Escherichia coli | |
6.1.1.4 | 7.5 | - |
L-methionine | ATP-diphosphate exchange reaction, wild-type enzyme, pH 7.8, 37°C | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.4 | ATP + L-leucine + tRNALeu | Escherichia coli | - |
AMP + diphosphate + L-leucyl-tRNALeu | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.1.1.4 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.1.1.4 | recombinant His-tagged mutant and His-tagged CP1 domain expressed in Escherichia coli strain Jm109(DE3), to electrophoretic homogeneity | Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.1.1.4 | 0.79 | - |
recombinant CP1 domain mutant | Escherichia coli |
6.1.1.4 | 1.5 | - |
about, wild-type enzyme | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.4 | ATP + L-isoleucine + tRNALeu | wild-type and CP1 domain mutant enzyme, the mischarged product can be edited by the wild-type enzyme, but not by a recombinant isolated CP1 domain | Escherichia coli | AMP + diphosphate + L-isoleucyl-tRNALeu | - |
? | |
6.1.1.4 | ATP + L-leucine + tRNALeu | - |
Escherichia coli | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
6.1.1.4 | ATP + L-leucine + tRNALeu | two-step reaction, the connecting peptide CP1 domain is crucial for the editing function | Escherichia coli | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
6.1.1.4 | ATP + L-methionine + tRNALeu | wild-type and CP1 domain mutant enzyme, the mischarged product can be edited by the wild-type enzyme, but not by a recombinant isolated CP1 domain | Escherichia coli | AMP + diphosphate + L-methionyl-tRNALeu | - |
? | |
6.1.1.4 | additional information | the enzyme also performs the ATP-diphosphate exchange reaction | Escherichia coli | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.1.1.4 | More | determination of secondary structure elements in the wild-type and mutant enzyme | Escherichia coli |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.1.1.4 | Leucine translase | - |
Escherichia coli |
6.1.1.4 | Leucine--tRNA ligase | - |
Escherichia coli |
6.1.1.4 | Leucyl-transfer ribonucleate synthetase | - |
Escherichia coli |
6.1.1.4 | Leucyl-transfer ribonucleic acid synthetase | - |
Escherichia coli |
6.1.1.4 | Leucyl-transfer RNA synthetase | - |
Escherichia coli |
6.1.1.4 | Leucyl-tRNA synthetase | - |
Escherichia coli |
6.1.1.4 | LeuRS | - |
Escherichia coli |
6.1.1.4 | Synthetase, leucyl-transfer ribonucleate | - |
Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.1.1.4 | 37 | - |
assay at | Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.4 | 1.3 | - |
tRNALeu | mutant enzyme, pH 7.8, 37°C | Escherichia coli | |
6.1.1.4 | 1.5 | - |
L-leucine | mutant enzyme, pH 7.8, 37°C | Escherichia coli | |
6.1.1.4 | 1.6 | - |
ATP | mutant enzyme, pH 7.8, 37°C | Escherichia coli | |
6.1.1.4 | 2.9 | - |
tRNALeu | wild-type enzyme, pH 7.8, 37°C | Escherichia coli | |
6.1.1.4 | 3 | - |
L-leucine | wild-type enzyme, pH 7.8, 37°C | Escherichia coli | |
6.1.1.4 | 3.6 | - |
ATP | wild-type enzyme, pH 7.8, 37°C | Escherichia coli | |
6.1.1.4 | 6.9 | - |
L-isoleucine | ATP-diphosphate exchange reaction, mutant enzyme, pH 7.8, 37°C | Escherichia coli | |
6.1.1.4 | 7.6 | - |
L-methionine | ATP-diphosphate exchange reaction, mutant enzyme, pH 7.8, 37°C | Escherichia coli | |
6.1.1.4 | 18 | - |
L-isoleucine | ATP-diphosphate exchange reaction, wild-type enzyme, pH 7.8, 37°C | Escherichia coli | |
6.1.1.4 | 19 | - |
L-methionine | ATP-diphosphate exchange reaction, wild-type enzyme, pH 7.8, 37°C | Escherichia coli | |
6.1.1.4 | 101 | - |
L-leucine | ATP-diphosphate exchange reaction, mutant enzyme, pH 7.8, 37°C | Escherichia coli | |
6.1.1.4 | 171 | - |
L-leucine | ATP-diphosphate exchange reaction, wild-type enzyme, pH 7.8, 37°C | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.1.1.4 | 7.8 | - |
assay at | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.4 | ATP | - |
Escherichia coli |