EC Number | Cloned (Comment) | Organism |
---|---|---|
7.2.4.2 | expressed in Escherichia coli DH5alpha/pSK-GAB | Klebsiella pneumoniae |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
7.2.4.2 | C291E | beta subunit, unstable in the absence of Na+, dissociates into an alpha-gamma subcomplex and the beta subunit | Klebsiella pneumoniae |
7.2.4.2 | D203N | beta subunit, complete loss of activity | Klebsiella pneumoniae |
7.2.4.2 | G337A | beta subunit, complete loss of activity | Klebsiella pneumoniae |
7.2.4.2 | G377A | beta subunit, complete loss of activity | Klebsiella pneumoniae |
7.2.4.2 | G380A | beta subunit, about 30% of activity | Klebsiella pneumoniae |
7.2.4.2 | N373L | beta subunit, almost complete loss of activity | Klebsiella pneumoniae |
7.2.4.2 | Q192L | beta subunit, lower pH optimum than wild type enzyme | Klebsiella pneumoniae |
7.2.4.2 | R389A | beta subunit, about 10% of activity, significantly decreased Na+ binding | Klebsiella pneumoniae |
7.2.4.2 | R389D | beta subunit, almost complete loss of activity | Klebsiella pneumoniae |
7.2.4.2 | R389K | beta subunit, about 80% of activity, significantly decreased Na+ binding | Klebsiella pneumoniae |
7.2.4.2 | R389L | beta subunit, about 10% of activity, significantly decreased Na+ binding | Klebsiella pneumoniae |
7.2.4.2 | S382A | beta subunit, complete loss of activity | Klebsiella pneumoniae |
7.2.4.2 | S382C | beta subunit, complete loss of activity | Klebsiella pneumoniae |
7.2.4.2 | S382D | beta subunit, about 10% of activity | Klebsiella pneumoniae |
7.2.4.2 | S382E | beta subunit, complete loss of activity | Klebsiella pneumoniae |
7.2.4.2 | S382N | beta subunit, complete loss of activity | Klebsiella pneumoniae |
7.2.4.2 | S382Q | beta subunit, complete loss of activity | Klebsiella pneumoniae |
7.2.4.2 | S382T | beta subunit, about 10% of activity | Klebsiella pneumoniae |
7.2.4.2 | Y209A | beta subunit, lower pH optimum than wild type enzyme | Klebsiella pneumoniae |
7.2.4.2 | Y229F | beta subunit, completely inactive | Klebsiella pneumoniae |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
7.2.4.2 | membrane | beta and gamma subunits are integral membrane proteins, alpha subunit is attached to the gamma subunit | Klebsiella pneumoniae | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
7.2.4.2 | Na+ | two ions per enzyme required for maximum activity, cooperative binding, 20-50 mM protects against tryptic digestion | Klebsiella pneumoniae | |
7.2.4.2 | Zn2+ | binds to the gamma subunit | Klebsiella pneumoniae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
7.2.4.2 | Oxaloacetate | Klebsiella pneumoniae | one Na+ is transported into the periplasm and one H+ is transported into the cytoplasm during the reaction | Pyruvate + CO2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
7.2.4.2 | Klebsiella pneumoniae | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
7.2.4.2 | recombinant protein from Escherichia coli | Klebsiella pneumoniae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
7.2.4.2 | additional information | - |
specific activity for all mutant enzymes | Klebsiella pneumoniae |
7.2.4.2 | 45 | - |
wild type enzyme | Klebsiella pneumoniae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
7.2.4.2 | Oxaloacetate | - |
Klebsiella pneumoniae | Pyruvate + CO2 | - |
? | |
7.2.4.2 | Oxaloacetate | one Na+ is transported into the periplasm and one H+ is transported into the cytoplasm during the reaction | Klebsiella pneumoniae | Pyruvate + CO2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
7.2.4.2 | trimer | alpha, beta, gamma | Klebsiella pneumoniae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
7.2.4.2 | 5.5 | - |
Q192L and Y209A mutants | Klebsiella pneumoniae |
7.2.4.2 | 6.5 | 7.5 | wild type enzyme | Klebsiella pneumoniae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
7.2.4.2 | biotin | binds to the C-terminal domain of the alpha-subunit | Klebsiella pneumoniae |