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Literature summary extracted from

  • Dolence, J.M.; Steward, L.E.; Dolence, E.K.; Wong, D.H.; Poulter, C.D.
    Studies with Recombinant Saccharomyces cerevisiae CaaX Prenyl Protease Rce1p (2000), Biochemistry, 39, 4096-4104.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.99.B1 expression in Saccharomyces cerevisiae Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
3.4.99.B1 C251A no proteolytic activity Saccharomyces cerevisiae
3.4.99.B1 E156A no proteolytic activity Saccharomyces cerevisiae
3.4.99.B1 H194A no proteolytic activity Saccharomyces cerevisiae
3.4.99.B1 H196A 50% of wild-type activity Saccharomyces cerevisiae
3.4.99.B1 H197A 8% of wild-type activity Saccharomyces cerevisiae
3.4.99.B1 H248A no proteolytic activity Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.99.B1 Ca2+ 0.087 mM, 97% inhibition Saccharomyces cerevisiae
3.4.99.B1 Co2+ 0.087 mM, 48% inhibition Saccharomyces cerevisiae
3.4.99.B1 Cu+ 0.087 mM, 97% inhibition Saccharomyces cerevisiae
3.4.99.B1 Mersalyl acid 0.022 mM, 99% inhibition Saccharomyces cerevisiae
3.4.99.B1 methyl methanothiosulfonate 0.05 mM, 40% inhibition Saccharomyces cerevisiae
3.4.99.B1 additional information not inhibited by protese inhibitors bestatin, antipain, chymostatin, pepstatin A, leupeptin, E64 and PMSF Saccharomyces cerevisiae
3.4.99.B1 N-Boc-Cys(farnesyl)-methylenamin-VIM-OH 0.0001 mM, 50% inhibition Saccharomyces cerevisiae
3.4.99.B1 Nalpha-tosyl-L-lysine chloromethyl ketone 0.3 mM, 32% inhibition Saccharomyces cerevisiae
3.4.99.B1 Nalpha-tosyl-L-phenylalanine chloromethyl ketone 0.3 mM, 94% inhibition Saccharomyces cerevisiae
3.4.99.B1 Ni2+ 0.087 mM, 48% inhibition Saccharomyces cerevisiae
3.4.99.B1 p-hydroxymercuribenzoic acid 0.022 mM, 99% inhibition Saccharomyces cerevisiae
3.4.99.B1 p-Hydroxymercuriphenylsulfonic acid 0.022 mM, 99% inhibition Saccharomyces cerevisiae
3.4.99.B1 Zn2+ 0.087 mM, 99% inhibition Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.99.B1 integral to membrane
-
 Saccharomyces cerevisiae
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.4.99.B1 Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.99.B1 dansyl-G(farnesyl)CIIS + H2O
-
 Saccharomyces cerevisiae dansyl-G(farnesyl)C + IIS
-
 ?
3.4.99.B1 dansyl-GLP(farnesyl)CVVM + H2O
-
 Saccharomyces cerevisiae dansyl-GLP(farnesyl)C + VVM
-
 ?
3.4.99.B1 dansyl-KTK(farnesyl)CVIM + H2O
-
 Saccharomyces cerevisiae dansyl-KTK(farnesyl)C + VIM
-
 ?
3.4.99.B1 dansyl-SAK(farnesyl)CVLS + H2O
-
 Saccharomyces cerevisiae dansyl-SAK(farnesyl)C + VLS
-
 ?
3.4.99.B1 dansyl-WDPA(farnesyl)CVIA + H2O
-
 Saccharomyces cerevisiae dansyl-WDPA(farnesyl)C + VIA
-
 ?
3.4.99.B1 dansyl-WDPA(geranylgeranyl)CVIA + H2O
-
 Saccharomyces cerevisiae dansyl-WDPA(geranylgeranyl)C + VIA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.99.B1 Ras and a-factor converting enzyme
-
 Saccharomyces cerevisiae
3.4.99.B1 Rce1p
-
 Saccharomyces cerevisiae