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Literature summary extracted from

  • Bergman, N.H.; Johnston, W.K.; Bartel, D.P.
    Kinetic framework for ligation by an efficient RNA ligase ribozyme (2000), Biochemistry, 39, 3115-3123.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.3 synthetic construct
-
 isolated from random sequences, pool of random sequences based on their ability to join a substrate oligonucleotide to their own 5'-terminus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.3 ATP + (ribonucleotide)n + (ribonucleotide)m ligates two substrate RNAs, promoting the attack of the 3'-hydroxyl of one substrate upon the 5'-triphosphate of the other substrate with release of diphosphate, reverse of ligation has been detected, but very inefficient, not saturable by soluble amounts of diphosphate synthetic construct AMP + diphosphate + (ribonucleotide)n+m
-
 ?
6.5.1.3 ATP + (RNA)n
-
 synthetic construct ?
-
 ?
6.5.1.3 ATP + RNA + 5'-aaCCAGUC DNA-RNA hybrid synthetic construct AMP + diphosphate + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.5.1.3 class I RNA ligase ribozyme
-
 synthetic construct

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.5.1.3 0.05
-
 (RNA)n pH 6.0, 22°C, multiple-turnover derivatives 210t and 207t, engineered by changing the arrangement of the base pairs in the P2 stem synthetic construct
6.5.1.3 0.266
-
 (RNA)n pH 8.0, 22°C, multiple-turnover derivative 207t, engineered by changing the arrangement of the base pairs in the P2 stem synthetic construct
6.5.1.3 0.583
-
 (RNA)n pH 9.0, 22°C, multiple-turnover derivative 207t, engineered by changing the arrangement of the base pairs in the P2 stem synthetic construct
6.5.1.3 2.33
-
 (RNA)n pH 8.0, 22°C, multiple-turnover derivative 210t, engineered by changing the arrangement of the base pairs in the P2 stem synthetic construct
6.5.1.3 2.94
-
 (RNA)n pH 8.0, 22°C, multiple-turnover derivative 210t, engineered by changing the arrangement of the base pairs in the P2 stem synthetic construct
6.5.1.3 6
-
 (RNA)n pH 9.0, 22°C, multiple-turnover derivative 210t, engineered by changing the arrangement of the base pairs in the P2 stem synthetic construct
6.5.1.3 6.08
-
 (RNA)n pH 9.0, 22°C, multiple-turnover derivative 207t, engineered by changing the arrangement of the base pairs in the P2 stem synthetic construct