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Literature summary extracted from

  • Barends, S.; Wower, J.; Kraal, B.
    kinetic parameters for tmRNA binding to alanyl-tRNA synthetase and elongation factor Tu from Escherichia coli (2000), Biochemistry, 39, 2652-2658.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.1.1.7 additional information elongation factor Tu has no stimulating effect on the enzyme with both substrates: tRNAAla and Ala-tmRNA, but it protects both acylated RNAs against hydrolysis of the alanyl ester bond Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.7 additional information
-
 additional information kinetics, ternary complex formation Escherichia coli
6.1.1.7 0.0015
-
 tRNAAla pH 7.6, 37°C, recombinant His-tagged enzyme Escherichia coli
6.1.1.7 0.024
-
 tmRNA pH 7.6, 37°C, recombinant His-tagged enzyme Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.7 Mg2+ required Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.7 96000
-
 1 * 96000, recombinant His-tagged enzyme, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.7 ATP + L-alanine + tRNAAla Escherichia coli
-
 AMP + diphosphate + L-alanyl-tRNAAla
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.7 Escherichia coli
-
 purified recombinant His-tagged enzyme
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.7 ATP + L-alanine + tmRNA transfer messenger RNA, 75fold reduced activity compared to cognate tRNAAla Escherichia coli AMP + diphosphate + L-alanyl-tmRNA
-
 ?
6.1.1.7 ATP + L-alanine + tRNAAla
-
 Escherichia coli AMP + diphosphate + L-alanyl-tRNAAla
-
 ?
6.1.1.7 ATP + L-alanine + tRNAAla 2-step reaction Escherichia coli AMP + diphosphate + L-alanyl-tRNAAla
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.7 monomer 1 * 96000, recombinant His-tagged enzyme, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
6.1.1.7 Ala-tRNA synthetase
-
 Escherichia coli
6.1.1.7 Alanine transfer RNA synthetase
-
 Escherichia coli
6.1.1.7 Alanine translase
-
 Escherichia coli
6.1.1.7 Alanine tRNA synthetase
-
 Escherichia coli
6.1.1.7 Alanine-transfer RNA ligase
-
 Escherichia coli
6.1.1.7 alanine-tRNA ligase
-
 Escherichia coli
6.1.1.7 Alanyl-transfer ribonucleate synthetase
-
 Escherichia coli
6.1.1.7 Alanyl-transfer ribonucleic acid synthetase
-
 Escherichia coli
6.1.1.7 Alanyl-transfer RNA synthetase
-
 Escherichia coli
6.1.1.7 Alanyl-tRNA synthetase
-
 Escherichia coli
6.1.1.7 AlaRS
-
 Escherichia coli
6.1.1.7 Synthase, alanyl-transfer ribonucleate
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.7 37
-
 assay at Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.7 0.043
-
 tmRNA pH 7.6, 37°C, 0.001 mg recombinant His-tagged enzyme Escherichia coli
6.1.1.7 0.2
-
 tRNAAla pH 7.6, 37°C, 0.00025 mg recombinant His-tagged enzyme Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.7 7.6
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.7 ATP
-
 Escherichia coli