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Literature summary extracted from

  • Wessel, P.M.; Graciet, E.; Douce, R.; Dumas, R.
    Evidence for two distinct effector-binding sites in threonine deaminase by site-directed mutagenesis, kinetic, and binding experiments (2000), Biochemistry, 39, 15136-15143.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.3.1.19 isoleucine activation below 0.01 mM, strong inhibition above, 50% inhibition at 0.064 mM Arabidopsis thaliana
4.3.1.19 NH4+ 2.1fold increase in activity Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.1.19 expression in Escherichia coli Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
4.3.1.19 Y449L concentration of isoleucine needed to reach 50% inhibition increases by a factor 45, two different effector-binding sites are constituted in part by Y449 and Y543 Arabidopsis thaliana
4.3.1.19 Y543L concentration of isoleucine needed to reach 50% inhibition increases by a factor 38, two different effector-binding sites are constituted in part by Y449 and Y543 Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.19 Ca2+
-
 Arabidopsis thaliana
4.3.1.19 isoleucine activation below 0.01 mM, strong inhibition above, 50% inhibition at 0.064 mM, inhibition can be reversed by valine Arabidopsis thaliana
4.3.1.19 Mg2+
-
 Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.3.1.19 K+ 3fold increase in activity, half-maximal activation at 3.1 mM Arabidopsis thaliana
4.3.1.19 Li+ 2.8fold increase in activity Arabidopsis thaliana
4.3.1.19 Na+ 1.7fold increase in activity Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.19 59800
-
 4 * 59800, SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.1.19 L-threonine Arabidopsis thaliana
-
 2-oxobutanoate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.19 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.19 recombinant threonine deaminase Arabidopsis thaliana

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.1.19 400
-
 recombinant threonine deaminase Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.19 L-threonine
-
 Arabidopsis thaliana 2-oxobutanoate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.1.19 tetramer 4 * 59800, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
4.3.1.19 TD
-
 Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.19 8
-
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
4.3.1.19 pyridoxal 5'-phosphate 1 mol per monomer Arabidopsis thaliana