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Literature summary extracted from
- Cloning, expression, and characterization of human cytosolic aminopeptidase P: a single manganese(II)-dependent enzyme (2000), Biochemistry, 39, 15121-15128.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.11.9 | glutathione | activates the enzyme in presence of Mn2+ or Co2+, and slightly in presence of Zn2+ | Homo sapiens |  |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.11.9 | DNA and amino acid sequence determination and analysis, expression in Escherichia coli BL21(DE3) as His-tagged enzyme, and in COS-1 cells | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.11.9 | 1,10-phenanthroline | complete inhibition at 0.1 mM | Homo sapiens | |
| 3.4.11.9 | apstatin | complete inhibition at 0.1 mM | Homo sapiens | |
| 3.4.11.9 | Ca2+ | 83.1% inhibition at 1 mM | Homo sapiens | |
| 3.4.11.9 | Co2+ | inhibitory at 1 mM | Homo sapiens | |
| 3.4.11.9 | Cu2+ | complete inhibition at 1 mM | Homo sapiens | |
| 3.4.11.9 | dithiothreitol | - |
Homo sapiens | |
| 3.4.11.9 | EDTA | 49% inhibition at 1 mM | Homo sapiens | |
| 3.4.11.9 | glutathione | 10% inhibition at 1 mM in absence of cations | Homo sapiens | |
| 3.4.11.9 | Mg2+ | 38.2% inhibition at 1 mM | Homo sapiens | |
| 3.4.11.9 | additional information | no significant inhibition by amastatin and enalaprilat | Homo sapiens | |
| 3.4.11.9 | Ni2+ | 93.5% inhibition at 1 mM | Homo sapiens | |
| 3.4.11.9 | Zn2+ | complete inhibition at 0.1 mM | Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.11.9 | 0.077 | - |
Substance P | pH 8.2, 37°C, recombinant wild-type enzyme | Homo sapiens | |
| 3.4.11.9 | 0.101 | - |
bradykinin | pH 8.2, 37°C, recombinant wild-type enzyme | Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.11.9 | cytosol | - |
Homo sapiens | 5829 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.11.9 | Co2+ | in a metal:protein ratio of 0.11:1, slightly activates the enzyme at 0.01-0.1 mM, 2.8fold activation at 1 mM in presence of 1 mM glutathione | Homo sapiens | |
| 3.4.11.9 | Fe2+ | in a metal:protein ratio of 0.07:1 | Homo sapiens | |
| 3.4.11.9 | Mn2+ | in a metal:protein ratio of 1:1, activates the enzyme 2.80fold at 0.1 mM with substrate substance P, maximal at 0.3 mM, 4.6fold activation at 1 mM in presence of 1 mM glutathione | Homo sapiens | |
| 3.4.11.9 | Zn2+ | in a metal:protein ratio of 0.11:1 | Homo sapiens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.9 | 71000 | - |
2 * 71000, recombinant enzyme, SDS-PAGE | Homo sapiens |
| 3.4.11.9 | 140000 | - |
approximately, gel filtration and native PAGE | Homo sapiens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.11.9 | Homo sapiens | Q9NQW7 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.11.9 | recombinant His-tagged enzyme from Ecscherichia coli | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.11.9 | leukocyte | - |
Homo sapiens | - |
| 3.4.11.9 | platelet | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.11.9 | bradykinin + H2O | hydrolysis of the N-terminal Arg1-Pro bond | Homo sapiens | Arg + des-Arg-bradykinin | - |
? | |
| 3.4.11.9 | additional information | no activity with Gly-Pro-hydroxyPro | Homo sapiens | ? | - |
? | |
| 3.4.11.9 | substance P + H2O | - |
Homo sapiens | Arg + des-Arg-substance P | - |
? | |
| 3.4.11.9 | substance P + H2O | - |
Homo sapiens | L-Arg + PKPQQFFGLM | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.11.9 | dimer | 2 * 71000, recombinant enzyme, SDS-PAGE | Homo sapiens |
| 3.4.11.9 | More | interaction of the subunits can not be disrupted by 2-mercaptoethanol, 1 M NaCl, 1% Triton X-100, and 4 mM CHAPS | Homo sapiens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.11.9 | aminopeptidase P | - |
Homo sapiens |
| 3.4.11.9 | AP-P | - |
Homo sapiens |
| 3.4.11.9 | More | the enzyme belongs to the peptidase family M24 | Homo sapiens |
| 3.4.11.9 | X-Pro aminopeptidase | - |
Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.9 | 37 | - |
assay at | Homo sapiens |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.11.9 | 3.1 | - |
Substance P | pH 8.2, 37°C, recombinant wild-type enzyme | Homo sapiens | |
| 3.4.11.9 | 3.13 | - |
bradykinin | pH 8.2, 37°C, recombinant wild-type enzyme | Homo sapiens | |
| 3.4.11.9 | 4.48 | - |
bradykinin | pH 8.2, 37°C, recombinant wild-type enzyme | Homo sapiens | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.9 | 8.2 | - |
recombinant enzyme expressed in Escherichia coli | Homo sapiens |
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