Literature summary extracted from
Radfar, R.; Leaphart, A.; Brewer, J.M.; Minor, W.; Odom, J.D.; Dunlap, R.B.; Lovell, C.R.; Lebioda, L.
Cation binding and thermostability of FTHFS monovalent cation binding sites and thermostability of N10-formyltetrahydrofolate synthetase from Moorella thermoacetica (2000), Biochemistry, 39, 14481-14486.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
6.3.4.3 |
expression in Escherichia coli |
Moorella thermoacetica |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
6.3.4.3 |
crystals of FTHFS complexed with NH4+, K+ or Cs+ are grown by vapor diffusion from 46% saturated ammonium sulfate, 1 mM dithiothreitol and 1% polyehtylene glycol 1000 in 50 mM potassium maleate buffer, pH 7.6, crystals diffract to 3.0-3.2 A resolution |
Moorella thermoacetica |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
6.3.4.3 |
E98D |
36% of wild-type activity |
Moorella thermoacetica |
6.3.4.3 |
E98Q |
93% of wild-type activity, slight increase in thermal stability in the absence of monovalent cation |
Moorella thermoacetica |
6.3.4.3 |
E98S |
61% of wild-type activity |
Moorella thermoacetica |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
6.3.4.3 |
K+ |
monovalent cation required for activity |
Moorella thermoacetica |
|
6.3.4.3 |
NH4+ |
monovalent cation required for activity |
Moorella thermoacetica |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
6.3.4.3 |
60000 |
- |
4 * 60000, SDS-PAGE |
Moorella thermoacetica |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
6.3.4.3 |
ATP + formate + tetrahydrofolate |
Moorella thermoacetica |
- |
ADP + phosphate + 10-formyltetrahydrofolate |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
6.3.4.3 |
Moorella thermoacetica |
P21164 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
6.3.4.3 |
recombinant FTHFS |
Moorella thermoacetica |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
6.3.4.3 |
780 |
- |
recombinant FTHFS |
Moorella thermoacetica |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
6.3.4.3 |
ATP + formate + tetrahydrofolate |
- |
Moorella thermoacetica |
ADP + phosphate + 10-formyltetrahydrofolate |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
6.3.4.3 |
tetramer |
4 * 60000, SDS-PAGE |
Moorella thermoacetica |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
6.3.4.3 |
FTHFS |
- |
Moorella thermoacetica |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
6.3.4.3 |
55 |
60 |
- |
Moorella thermoacetica |
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
6.3.4.3 |
69 |
- |
in the absence of monovalent cation |
Moorella thermoacetica |
6.3.4.3 |
79 |
- |
in the presence of 200 mM K+ |
Moorella thermoacetica |