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Literature summary extracted from

  • Gamper, M.; Hilvert, D.; Kast, P.
    Probing the role of the C-terminus of Bacillus subtilis chorismate mutase by a novel random protein-termination strategy (2000), Biochemistry, 39, 14087-14094.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.5 expression in Escherichia coli Bacillus subtilis

Protein Variants

EC Number Protein Variants Comment Organism
5.4.99.5 DELTA117-127 large increase in KM and slower turnover relative to wild-type enzyme Bacillus subtilis
5.4.99.5 DELTA118-127 large increase in KM and slower turnover relative to wild-type enzyme Bacillus subtilis
5.4.99.5 DELTA118-127/K111N/A112S/V113N large increase in KM and slower turnover relative to wild-type enzyme Bacillus subtilis
5.4.99.5 DELTA118-127/R116L/P117T large increase in KM and slower turnover relative to wild-type enzyme Bacillus subtilis
5.4.99.5 DELTA119-127/D118N large increase in KM and slower turnover relative to wild-type enzyme Bacillus subtilis
5.4.99.5 R90Q complete inactivation of enzyme Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.99.5 0.081
-
 chorismate 30°C, pH 7.5, wild-type, with 4 mM substrate Bacillus subtilis
5.4.99.5 3.9
-
 chorismate 30°C, pH 7.5, mutant DELTA118-127, with 5.8 mM substrate Bacillus subtilis
5.4.99.5 4
-
 chorismate 30°C, pH 7.5, mutant DELTA119-127/D118N, with 5.8 mM substrate Bacillus subtilis
5.4.99.5 4.1
-
 chorismate 30°C, pH 7.5, mutant DELTA118-127, with 3.4 mM substrate Bacillus subtilis
5.4.99.5 9.3
-
 chorismate 30°C, pH 7.5, mutant DELTA117-127, with 3.8 mM substrate Bacillus subtilis
5.4.99.5 15
-
 chorismate 30°C, pH 7.5, mutant DELTA118-127/K111N/A112S/V113N, with 4 mM substrate Bacillus subtilis
5.4.99.5 16
-
 chorismate 30°C, pH 7.5, mutant DELTA118-127/R116L/P117T, with 3.9 mM substrate Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.99.5 Chorismate Bacillus subtilis biosynthesis of aromatic amino acids Prephenate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.5 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.99.5
-
 Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.5 Chorismate
-
 Bacillus subtilis Prephenate
-
 ?
5.4.99.5 Chorismate biosynthesis of aromatic amino acids Bacillus subtilis Prephenate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.4.99.5 BsCM
-
 Bacillus subtilis
5.4.99.5 chorismate mutase
-
 Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.4.99.5 22
-
 chorismate 30°C, pH 7.5, mutant DELTA118-127, with 5.8 mM substrate Bacillus subtilis
5.4.99.5 23
-
 chorismate 30°C, pH 7.5, mutant DELTA119-127/D118N, with 5.8 mM substrate Bacillus subtilis
5.4.99.5 24
-
 chorismate 30°C, pH 7.5, mutant DELTA118-127, with 3.4 mM substrate Bacillus subtilis
5.4.99.5 26
-
 chorismate 30°C, pH 7.5, mutant DELTA117-127, with 3.8 mM substrate and DELTA 118-127/R116L/P117T, with 3.9 mM substrate Bacillus subtilis
5.4.99.5 30
-
 chorismate 30°C, pH 7.5, mutant DELTA118-127/K111N/A112S/V113N, with 4 mM substrate Bacillus subtilis
5.4.99.5 47
-
 chorismate 30°C, pH 7.5, wild-type, with 4 mM substrate Bacillus subtilis