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Literature summary extracted from

  • Jackson, L.K.; Brooks, H.B.; Osterman, A.L.; Goldsmith, E.J.; Phillips, M.A.
    Altering the reaction specificity of eukaryotic ornithine decarboxylase (2000), Biochemistry, 39, 11247-11257.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.17 expression in Escherichia coli Trypanosoma brucei

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.17 ODC complexed with putrescine, 25 mg/ml ODC in 10 mM HEPES, pH 7.2, 450 mM NaCl, 10 mM dithiothreitol, 0.5 mM EDTA, 0.01% Brij-20, are preincubated with 15 mM D-ornithine for 10 min at room temperatur before crystallization, crystals are obtained at 16°C by vapor diffusion mixing equal volumes of ODC-D-ornithine mixture and well solution containing 20% polyethylene glycol 3350, 200 mM NaOAc, 100 mM HEPES, pH 7.5 and 10 mM dithiothreitol Trypanosoma brucei

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.17 C360A 2% of wild-type activity Trypanosoma brucei
4.1.1.17 C360S 0.1% of wild-type activity Trypanosoma brucei

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.17 alpha-difluoromethylornithine
-
 Trypanosoma brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.17 0.054
-
 L-ornithine C360S mutant Trypanosoma brucei
4.1.1.17 0.1
-
 L-ornithine C360A mutant Trypanosoma brucei
4.1.1.17 0.4
-
 L-ornithine
-
 Trypanosoma brucei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.17 L-ornithine Trypanosoma brucei
-
 putrescine + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.17 Trypanosoma brucei P07805
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.17 recombinant ODC Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.17 L-ornithine
-
 Trypanosoma brucei putrescine + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.17 ODC
-
 Trypanosoma brucei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.17 15
-
 L-ornithine
-
 Trypanosoma brucei

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.17 pyridoxal 5'-phosphate
-
 Trypanosoma brucei