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Literature summary extracted from

  • Thompson, T.B.; Garrett, J.B.; Taylor, E.A.; Meganathan, R.; Gerlt, J.A.; Rayment, I.
    Evolution of enzymatic activity in the enolase superfamily: structure of o-succinylbenzoate synthase from Escherichia coli in complex with Mg2+ and o-succinylbenzoate (2000), Biochemistry, 39, 10662-10676.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.113 expression of a recombinant enzyme in Escherichia coli Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.113 crystallization of the apoenzyme and a complex with Mg2+ and 4-(2-carboxyphenyl)-4-oxobutyrate Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.113 Mg2+ essential for activity Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.113 35760
-
 mass spectrometry Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.113 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylic acid Escherichia coli
-
 4-(2-carboxyphenyl)-4-oxobutyrate + H2O
-
 ir
4.2.1.113 additional information Escherichia coli involved in the menaquinone biosynthetic pathway ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.113 Escherichia coli P29208
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.113 purification of a recombinant His-tagged enzyme by affinity chromatography followed by tag cleavage and anion-exchange chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.113 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylic acid
-
 Escherichia coli 4-(2-carboxyphenyl)-4-oxobutyrate + H2O
-
 ?
4.2.1.113 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylic acid
-
 Escherichia coli 4-(2-carboxyphenyl)-4-oxobutyrate + H2O
-
 ir
4.2.1.113 additional information involved in the menaquinone biosynthetic pathway Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.113 OSBS
-
 Escherichia coli